Aminopeptidase N‐Activated Self‐immolative Hydrogen Sulfide Donor for Inflammatory Response‐Specific Wound Healing

Angewandte Chemie International Edition, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Abstract Hydrogen sulfide (H 2 S) plays crucial inflammatory modulating roles, representing a promising candidate for anti‐inflammatory therapies. However, current H S delivery approaches lack sufficient specificity against response. Herein, regarding the overexpressed aminopeptidase N (APN) at inflammation sites, an APN‐activated self‐immolative carbonyl (COS)/H donor ( AlaCOS ) was developed response‐specific delivery. The compound showed sustained generation upon APN activation in presence of carbonic anhydrase (CA), and responsiveness could be well regulated by amino acid sequence. Due to delivery, provided potent capability, which further validated RNA sequencing. In vivo experiments on full‐thickness cutaneous wound murine model also strong promoting effect healing, mainly due regulation response . By introducing caged coumarin fluorophore molecular architecture, self‐reporting fluorescence generated accompanied with APN‐mediated COS/H release, achieved visualization vitro vivo. This work not only offers useful tool studying bioactivity inflammation, but provides new insights developing novel therapies cope inflammation‐associated diseases.

Language: Английский

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Concurrent Subcellular Delivery of Hydrogen Sulfide and a Payload with Near-Infrared Light

JACS Au, Journal Year: 2024, Volume and Issue: 4(7), P. 2687 - 2694

Published: July 5, 2024

Hydrogen sulfide (H2S) is a gaseous signaling molecule, exerting crucial regulatory functions in organelles and cellular environments. H2S exhibits high therapeutic potential synergistic effects with other drugs, its potency notably enhanced through organelle-specific targeting. Yet, the navigation of light-activated donors to specific remains absent. Here, we report first photocage that simultaneously delivers payload subcellular precision mitochondria live human cells using tissue-penetrating near-infrared light as trigger. The fluorogenic enables real-time monitoring process, demonstrate concurrent uncaging combination fluorescence microscopy mitochondria-specific fluorescent probes. We anticipate these photocages will permit precise delivery H2S-drug combinations exceptional spatiotemporal control, thereby driving harnessing known discovery novel strategies.

Language: Английский

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Development of a Photoelectrochemical Microelectrode Using an Organic Probe for Monitoring Hydrogen Sulfide in Living Brains

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(49), P. 19822 - 19832

Published: Nov. 22, 2024

Hydrogen sulfide (H2S) is an important bioactive molecule that plays a significant role in various functions, particularly the living brain, where it closely linked to cognition, memory, and several neurological diseases. Consequently, developing effective detection methods for H2S essential studying brain functions underlying mechanisms of these This study aims construct novel photoelectrochemical (PEC) microelectrode Ti/TiO2@HSP quantitative monitoring levels brain. The PEC formed by covalently bonding specifically designed organic probe HSP, which possesses D-π–A structure, surface TiO2 nanotubes generated via situ anodic oxidation titanium wire. HSP can effectively react with generate photocurrent response under long-wavelength excitation light (560 nm), thereby achieving H2S. sensor demonstrates high sensitivity good selectivity. In vivo experiments utilizing enable dynamic changes across regions mouse findings reveal normal mice, concentration hippocampus significantly higher than striatum cerebral cortex. Additionally, following propargylglycine drug stimulation, concentrations different were observed decrease, most substantial reduction noted hippocampus. suggests cystathionine γ-lyase (CSE) primary enzyme responsible production this area, while exhibits less pronounced decrease concentration, indicating reliance on alternative enzymatic pathways production. Therefore, not only successfully develops high-performance but also provides new experimental tools theoretical foundations further exploring roles neurophysiological pathological processes.

Language: Английский

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A thiocoumarin based self-reporting sulfide prodrug strategy with a favorable safety profile

European Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: 289, P. 117426 - 117426

Published: Feb. 20, 2025

Language: Английский

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Fast Intramolecular Thiol-Activated Arylselenoamides Provide Access to Triggered, Fluorogenic H₂Se Donors

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(36), P. 24776 - 24781

Published: Aug. 26, 2024

H

Language: Английский

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Synthesis of Unsymmetrical Disulfides via Photocatalytic Hydrodisulfuration

ACS Catalysis, Journal Year: 2024, Volume and Issue: unknown, P. 18237 - 18246

Published: Nov. 26, 2024

Language: Английский

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Research Progress in the Field of Hydrogen Sulfide Donors in the Last Five Years

ChemBioChem, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Hydrogen sulfide (H

Language: Английский

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A cysteine-activated fluorescent H2S donor for visualizing H2S release and alleviating cellular inflammation

Chemical Communications, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

We developed a Cys-activated fluorescent H 2 S donor, Bcy-NCS, which releases and enables visualization through fluorescence changes, while also alleviating cellular inflammation.

Language: Английский

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