Recent Advancements in the Biomanufacturing of Crocetin and Crocins: Key Enzymes and Metabolic Engineering DOI
Chengpeng Hao, Kai Hu, Jingcong Xie

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Crocetin and crocins are high-value apocarotenoids recognized for their role as food colorants well numerous industrial therapeutic applications. Biotechnological platforms have the potential to replace traditional plant-based extraction of these compounds with a more sustainable approach. This review first introduced catalytic characteristics key enzymes involved in biosynthetic pathway crocetin crocins, including carotenoid cleavage dioxygenases, aldehyde dehydrogenases, uridine diphosphate glycosyltransferases. Next, we highlighted advanced metabolic engineering strategies aimed at enhancing crocin production, such increasing pool precursors cofactors, protein mining engineering, tuning expression, biosensor, genomic integration, process optimization. Finally, paper proposed tools associated further boosting heterologous production meet commercial-scale demands.

Language: Английский

Recent Advancements in the Biomanufacturing of Crocetin and Crocins: Key Enzymes and Metabolic Engineering DOI
Chengpeng Hao, Kai Hu, Jingcong Xie

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 8, 2025

Crocetin and crocins are high-value apocarotenoids recognized for their role as food colorants well numerous industrial therapeutic applications. Biotechnological platforms have the potential to replace traditional plant-based extraction of these compounds with a more sustainable approach. This review first introduced catalytic characteristics key enzymes involved in biosynthetic pathway crocetin crocins, including carotenoid cleavage dioxygenases, aldehyde dehydrogenases, uridine diphosphate glycosyltransferases. Next, we highlighted advanced metabolic engineering strategies aimed at enhancing crocin production, such increasing pool precursors cofactors, protein mining engineering, tuning expression, biosensor, genomic integration, process optimization. Finally, paper proposed tools associated further boosting heterologous production meet commercial-scale demands.

Language: Английский

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