Novel NMR-Based Approach to Reveal the ‘Metabolic Fingerprint’ of Cytotoxic Gold Drugs in Cancer Cells

Journal of Proteome Research, Journal Year: 2025, Volume and Issue: 24(2), P. 813 - 823

Published: Jan. 6, 2025

A combination of pathway enrichment and metabolite clustering analysis is used to interpret untargeted 1H NMR metabolomics data, enabling a biochemically informative comparison the effects induced by panel known cytotoxic gold(I) gold(III) compounds in A2780 ovarian cancer cells. The identification most dysregulated pathways for major classes highlights specific chemical features that lead common biological effects. proposed approach may have broader applicability screening metal-based drug candidate libraries, which always complicated their multitarget nature, support comprehensive interpretation metabolic actions.

Language: Английский

The X-ray structure of the adduct formed upon reaction of aurothiomalate with apo-transferrin: gold binding sites and a unique transferrin structure along the apo/holo transition pathway

Inorganic Chemistry Frontiers, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

The interaction of aurothiomalate with the apo-form transferrin (hTF) was studied by X-ray crystallography. Data provide information on gold binding sites and a unique structure along apo-hTF/holo-hTF transition pathway.

Language: Английский

Screening and Functional Characterization of Nanobodies Targeting the Transferrin Receptor

Protein Expression and Purification, Journal Year: 2025, Volume and Issue: unknown, P. 106702 - 106702

Published: March 1, 2025

Language: Английский