An aptazyme-based logic gate biosensor for tumor detection in postoperative analysis

Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137594 - 137594

Published: March 1, 2025

Language: Английский

Modular Engineering of the DNAzyme-Based Electrochemiluminescence Biosensor with the Nitrogen and Sulfur Codoped Carbon Dots as Near-Infrared Emitters for MicroRNA detection

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 29, 2025

DNAzyme-based biosensors remain at the forefront of microRNA (miRNA) analysis efforts. In this work, an electrochemiluminescence (ECL) biosensor technology integrating miRNA-initiated DNAzyme with a near-infrared ECL emission system was designed to detect miRNA. The through rational reconstitution Mg2+-specific 8-17 DNAzyme. We engineered strand incorporation blocker inhibit activity. formed duplex structure that prohibited hybridization one arm substrate strand, leading formation inactive structure. presence target miRNA, between miRNA and would induce breakage duplex, followed by active Thus, activity could be switched from OFF state into ON state. Moreover, nitrogen sulfur codoped carbon dots (NIR NS-CDs) were used as neoteric emitters, which not only possessed NIR reduce photochemical damage but also had low excitation potential range avoid side reaction. Therefore, combining modular engineering strategy NS-CDs constructed realize ultrasensitive miRNA-155 identification limit 58.5 aM. This DNAzyme-guided CD provides sensitive reliable method for detection, it has great in clinical diagnosis.

Language: Английский

Hydrogel empowered extracellular vesicles isolation, detection, and delivery

Nano Today, Journal Year: 2025, Volume and Issue: 64, P. 102817 - 102817

Published: May 28, 2025

Language: Английский