Nano Today, Journal Year: 2025, Volume and Issue: 64, P. 102817 - 102817
Published: May 28, 2025
Language: Английский
Nano Today, Journal Year: 2025, Volume and Issue: 64, P. 102817 - 102817
Published: May 28, 2025
Language: Английский
Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: unknown, P. 137594 - 137594
Published: March 1, 2025
Language: Английский
Citations
0Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown
Published: April 29, 2025
DNAzyme-based biosensors remain at the forefront of microRNA (miRNA) analysis efforts. In this work, an electrochemiluminescence (ECL) biosensor technology integrating miRNA-initiated DNAzyme with a near-infrared ECL emission system was designed to detect miRNA. The through rational reconstitution Mg2+-specific 8-17 DNAzyme. We engineered strand incorporation blocker inhibit activity. formed duplex structure that prohibited hybridization one arm substrate strand, leading formation inactive structure. presence target miRNA, between miRNA and would induce breakage duplex, followed by active Thus, activity could be switched from OFF state into ON state. Moreover, nitrogen sulfur codoped carbon dots (NIR NS-CDs) were used as neoteric emitters, which not only possessed NIR reduce photochemical damage but also had low excitation potential range avoid side reaction. Therefore, combining modular engineering strategy NS-CDs constructed realize ultrasensitive miRNA-155 identification limit 58.5 aM. This DNAzyme-guided CD provides sensitive reliable method for detection, it has great in clinical diagnosis.
Language: Английский
Citations
0Nano Today, Journal Year: 2025, Volume and Issue: 64, P. 102817 - 102817
Published: May 28, 2025
Language: Английский
Citations
0