Protein‐Interference‐Free and Kidney‐Targeting NIR Fluorophores for Accurate in Vivo Imaging of H₂S₂ during Kidney Ferroptosis

Advanced Healthcare Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

High-fidelity kidney function imaging is important for assessing the nephrotoxicity of drugs and diagnosing renal diseases. However, current challenges in achieving accurate include unspecific signal enhancement due to albumin binding relatively low distribution agents kidneys. Here, first time, a side-chain engineering strategy that incorporates hydrophilic six-membered heterocycles into aza-hemicyanine generating high-performance with protein-interference-free kidney-targeting features proposed. Based on these unique dyes, albumin-insensitive H2S2 near-infrared (NIR) fluorescent probe NA-H2S2 designed, which demonstrates effective following intravenous injection specifically activated by H2S2. The designed presents highly rapid, selective sensitive response detection limit as 24.21 nm. Additionally, it successfully achieves real-time vivo NIR fluorescence during erastin/cisplatin induced ferroptosis. Moreover, also enables rapid through vitro optical urinalysis, offering significant diagnostic value Overall, this study not only practical increased accuracy but provides promising dyes further development disease-related probes.

Language: Английский

Self-Reporting Ratiometric AIEgen-Peptide Nanoprobes for Activatable Chemotherapy and Noninvasive Imaging of Therapeutic Outcomes

Journal of Medicinal Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Efficacious chemotherapy and real-time therapeutic monitoring remain major challenges in cancer treatment. Traditional systems often lack tumor specificity, limiting efficacy, hindering therapy optimization. Moreover, the absence of can lead to missed opportunities increased risks side effects. Herein, we designed a self-reporting ratiometric AIEgen-peptide nanoprobe (TPE-1(Hyd-DOX)-DEVD) for activatable noninvasive imaging outcomes. When doxorubicin (DOX) is selectively activated acidic microenvironment, ensuing caspase-3 cascade triggers morphological transformation that amplifies TPE fluorescence. This enhancement allows TPE/DOX fluorescence ratio serve as an indicator DOX activation providing feedback. Both vitro vivo studies demonstrated TPE-1(Hyd-DOX)-DEVD exhibited impressive suppression efficacy excellent biocompatibility. study highlights strong potential this valuable tool theranostics, offering hope more effective personalized treatment strategies.

Language: Английский

A photostable luminescent iridium(III) complex probe for imaging endogenous mitochondrial sulfur dioxide in living cells

Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy, Journal Year: 2025, Volume and Issue: unknown, P. 126147 - 126147

Published: March 1, 2025

Language: Английский