Scaffold-Adaptive P450 Enzymes Balance Substrate Promiscuity with Catalytic Specificity in Brassicicene Biosynthesis DOI

Zhe Chen,

Wenling Yuan,

Fengli Li

et al.

Organic Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

We investigated the functional plasticity of two P450 enzymes, AbnK and AbnG, in brassicicene biosynthetic pathway. Through vivo heterologous expression, feeding assays, vitro reactions, we show that these enzymes regio- stereoselectively transform both 5–8–5 5–9–5 terpenoid scaffolds different ways, supported by computational simulations. also bridges a missing step A biosynthesis. Our findings demonstrate how controlled promiscuity underpins structural diversity, informing strategies to expand chemoenzymatic synthesis.

Language: Английский

Scaffold-Adaptive P450 Enzymes Balance Substrate Promiscuity with Catalytic Specificity in Brassicicene Biosynthesis DOI

Zhe Chen,

Wenling Yuan,

Fengli Li

et al.

Organic Letters, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

We investigated the functional plasticity of two P450 enzymes, AbnK and AbnG, in brassicicene biosynthetic pathway. Through vivo heterologous expression, feeding assays, vitro reactions, we show that these enzymes regio- stereoselectively transform both 5–8–5 5–9–5 terpenoid scaffolds different ways, supported by computational simulations. also bridges a missing step A biosynthesis. Our findings demonstrate how controlled promiscuity underpins structural diversity, informing strategies to expand chemoenzymatic synthesis.

Language: Английский

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