Ultrasmall platinum single-atom enzyme alleviates oxidative stress and macrophage polarization induced by acute kidney ischemia–reperfusion injury through inhibition of cell death storm

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 27, 2025

Acute kidney injury (AKI), characterized by a rapid decline in renal function, is associated with impaired mitochondrial function and excessive reactive oxygen species (ROS). Therefore, the exploration of ROS scavengers provides promising new opportunities for prevention treatment AKI mitigating oxidative stress. Here, we construct an ultrasmall platinum single-atom enzyme (Pt/SAE) multiple antioxidant activities to protect against acute ischemia-reperfusion (I/R) injury. Pt/SAE not only mimics superoxide dismutase catalase convert anion into water oxygen, but also exhibits impressive hydroxyl radical scavenging capacity, thereby reducing pro-inflammatory macrophage levels preventing inflammation. Furthermore, reduces accumulation Z-form DNA, which excessively accumulates following I/R damage, thus decreasing its interaction Z-DNA binding protein 1, consequently progression PANoptosis Additionally, downregulation induced suppresses lipid peroxidation, return ferroptosis I/R. Both vitro vivo experiments confirm that effectively mitigates inflammatory cell infiltration promotes shift polarization from M1-like M2-like subtype. This study information development novel SAEs as viable method AKI.

Language: Английский

Fucoxanthin from Laminaria japonica Targeting PANoptosis and Ferroptosis Pathways: Insights into Its Therapeutic Potential Against Ovarian Cancer

Marine Drugs, Journal Year: 2025, Volume and Issue: 23(3), P. 123 - 123

Published: March 12, 2025

Ovarian cancer (OC) is a highly aggressive malignancy with poor prognosis, necessitating novel therapeutic strategies. Fucoxanthin (FX), marine-derived carotenoid from Laminaria japonica, has demonstrated promising anticancer potential. This study revealed that FX exerts multiple effects in OC by inhibiting cell proliferation, invasion, and migration, while inducing various forms of programmed death (PCD). triggered PANoptosis (apoptosis, necroptosis, pyroptosis) ferroptosis. treatment regulated key markers associated PANoptosis, including apoptosis (Bcl-2, cleaved caspase-3), pyroptosis (GSDME), necroptosis (RIPK3). Additionally, modulated ferroptosis-related markers, such as SLC7A11 GPX4, increasing reactive oxygen species (ROS) Fe2+ levels disrupting mitochondrial function. Proteomic molecular docking analyses identified AMP-activated protein kinase (AMPK) direct target, activating the AMPK/Nrf2/HMOX1 pathway to promote In vivo, significantly reduced tumor growth xenograft models, accompanied enhanced ferroptosis marker expression. These findings demonstrate induces through promotes via distinct mechanisms, highlighting its potential agent for OC.

Language: Английский