Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 26, 2025
Developing
sensitive
and
highly
active
nanozymes
for
antioxidant
analysis
is
of
the
utmost
significance
in
medical
diagnosis
health
monitoring
due
to
their
essential
roles
as
free
reactive
oxygen
species
scavengers.
Here,
six
metal-organic
frameworks
(MOFs)-based
are
developed
a
dual-mode
absorbance/image
colorimetric
sensor
array
simultaneous
discrimination
determination
various
antioxidants
with
comparable
structural
or
chemical
properties.
The
catalysts
exhibit
wide
range
potent
oxidase-like
catalytic
activities,
verified
by
kinetic
parameters,
presence
dispersed
transition
metallic
bimetallic
redox
nodes.
These
efficiently
catalyze
oxidation
colorless
3,3',5,5'-tetramethylbenzidine
(TMB)
blue
TMBox,
resulting
noticeable
absorption
RGB
color
changes
at
650
nm.
Various
demonstrate
different
reducing
capabilities
leading
generation
fingerprint-like
spectral
patterns.
pattern
recognition
chemometrics
methods
including
principal
component
(PCA)
linear
discriminant
(LDA)
represent
well-separated
clustering
ascorbic
acid,
dopamine,
uric
cysteine,
glutathione,
thiocyanate,
tannic
gallic
acid.
assay
provides
detection
(0.1-75
μM)
limits
low
30
nM.
successfully
discriminated
concentrations,
mixtures,
interferences.
Furthermore,
sensor's
applicability
biologically
relevant
was
validated
urine
plasma
samples.
Overall,
MOF-based
nanozyme
offers
promising
platform
discriminating
determining
potential
applications.
ACS Sensors,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 6, 2025
The
development
of
DNA
rolling
machines
with
high
efficiency
for
ratiometric
biosensing
is
great
significance
the
accurate
diagnosis
and
evaluation
diseases.
Herein,
an
interparticle
machine
constructed
by
well-oriented
ordered
nanorollers
guided
tetrahedral
was
exploited
high-efficiency
lung
tumor-related
human
neutrophil
elastase
(HNE)
SERS
sensing.
In
this
design,
blocked
DNAzyme
assembled
on
AuNPs
to
engineer
walking
(WDNs)
endowed
collision
accessibility,
significantly
improving
reaction
kinetics
efficiency.
When
"leg
DNA"
WDNs
were
activated
through
multivalent
converted
target
HNE,
polyoriented
walkers
rolled
efficiently
along
directional
hairpin
"track
magnetic
NPs
(H1@MNPs)
that
served
as
continuously
"cleaving-rolling-assembly"
specific
substrates
(CRAS),
constructing
finally
converting
HNE
into
a
signal
in
nanogap-rich
silver
nanoisland
substrate.
CRAS
increased
direction
local
concentration,
accessibility
signal.
relative
motion
nonplanar
arms
weakens
derailment
DNA",
improves
processivity,
amplifies
capability.
Moreover,
promote
formation
high-density
hot
spot
domains,
further
detection
sensitivity.
Of
note,
biosensor
successfully
measures
limit
0.25
pM
can
screen
inhibitors
discriminate
levels
serum
tissue
normal
tumor
patients,
suggesting
provides
effective
tool
early
diagnosis,
prognostic
evaluation,
drug
discovery
tumor.
New Journal of Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
ECNA
as
chemodosimetric
fluorescence
‘turn-off’
probe
for
detection
of
hydrazine
with
significant
advantages
operational
simplicity,
spray
analysis,
and
spot
test
LOD
=
3.077
nM
LOQ
10.256
nM.
Small,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 14, 2025
Abstract
High
catalytic
efficiency
is
persistently
pursued
to
generate
high‐performance
nanozymes.
Metal
elements
are
active
centers
where
metal–organic
frameworks
(MOFs)
play
a
role.
In
this
work,
library
of
MOFs‐derived
nanozyme
constructed
based
on
metal
salts
and
1
H
‐1,2,3‐triazole
(MET).
Triazole
has
three
N
atoms;
the
intrinsic
high
content
beneficial
improve
affinity
substrate
2
O
.
Meanwhile,
different
kinds
metals
can
be
introduced
by
changing
salts,
through
which
bimetallic
or
trimetallic
MOFs
constructed.
Amongst
various
single–,
bi‐,
MET
nanozymes,
MET‐Fe
Zn
0.5
exhibits
best
peroxidase
(POD)‐like
activity.
The
results
demonstrate
that
introduction
accelerates
electron
transfer
promotes
conversion
Fe
3+
2+
in
nanozyme,
thus
enhancing
POD‐like
activity
nanozyme.
particular,
excellent
antibacterial
efficiency.
A
study
mechanism
indicates
good
adhesion
bacterial
membrane
work
conjunction
with
reactive
oxygen
species,
disrupting
oxidative
phosphorylation,
DNA
replication,
biosynthesis
essential
amino
acids
cofactors
within
bacteria,
leading
damage
and,
eventually
death
bacteria.
These
suggest
N‐coordination
number
great
potential
as
new‐generation
