Analytical Chemistry,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 25, 2025
The
judicious
utilization
of
antibiotics
has
established
a
robust
bulwark
for
human
health.
However,
their
improper
usage
engendered
deleterious
ramifications
on
the
environment,
underscoring
imperative
developing
efficacious
and
cost-effective
detection
degradation
platforms.
This
study
presents
sulfur-modified
iron-cobalt
bimetallic
single-atom
nitrogen-doped
carbon
catalyst
(S-FeCo-NC)
with
noncopper
active
center.
In
contrast
to
conventional
laccase,
which
utilizes
copper
as
its
center,
S-FeCo-NC
exhibits
multiple
enzyme
activities,
including
laccase-like,
peroxidase-like,
catalase-like
functions,
iron
cobalt
serving
centers.
As
proof
concept,
combined
laccase-like
functions
were
used
independent
signal
outputs,
while
multienzyme
cascade
dual-mode
assay
system
was
designed
rapid
tetracycline
(TC)
in
combination
peroxidase-like
enzymes.
this
system,
oxygen
directly
participated
catalytic
process
an
electron
acceptor,
peroxidase
efficiently
catalyzed
production
O2
from
H2O2.
elevated
concentration
offered
unique
advantage
increased
activity
enzyme,
outputs
visually
resolved
colorimetric
signals
using
stable
4-aminopyridine
oxidized
TC.
Furthermore,
generation
OH
radicals
strong
oxidative
properties,
these
carried
out
effective
decomposition
output
response
performed
differential
pulse
cyclic
voltammetry,
further
improved
sensitivity
accuracy
detection.
experimental
findings
demonstrate
that
favorable
TC
within
range
0.005-500
μM,
reaching
0.5-500
0.005-1.00
respectively,
limit
is
low
0.22
μM
1.68
nM,
respectively.
based
activity,
been
shown
significantly
enhance
also
demonstrating
stability
lower
range.
suggests
it
may
offer
novel
approach
sensitive
environmental
pollutants.
Analytical Methods,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
In
this
paper,
poly(1,4-benzenedithiol)
nanoparticles
(PBDT)
were
used
to
support
AuNPs.
Cardiac
troponin
I
(cTnI)
was
as
the
model
analyte
verify
efficacy
and
reliability
of
PBDT@AuNPs
LFIA
test
strips.
