Insects, Journal Year: 2023, Volume and Issue: 14(5), P. 418 - 418
Published: April 27, 2023
The Colorado potato beetle,
Language: Английский
Agronomy, Journal Year: 2025, Volume and Issue: 15(2), P. 358 - 358
Published: Jan. 30, 2025
The South American tomato pinworm, Tuta absoluta (Meyrick), is one of the major invasive pests that causes severe economic damage to several hosts, especially plants, globally. Spinosad, a biopesticide widely used against insect pests, also shows promising results T. absoluta. Here, we investigated evolution spinosad resistance and its effects on biological traits related gene fully understand relationship between degree associated fitness costs. spinosad-resistant strain (SpRS) with moderate level (14.40-fold) was developed following continuous selection susceptible (SS) for eight generations. Fitness analysis showed larval pupal developmental durations were significantly increased, while adult emergence substantially reduced in SpRS compared SS. Adult longevity (male female), fecundity, hatching rates decreased Additionally, expression analyses indicated down-regulation development reproduction-related genes (Vg, VgR, JHBP, JHAMT, JHE, JHDK) population. Further, mRNA resistance-related cytochrome P450 CYP321C40, followed by CYP4M116, CYP6AW1, CYP339A1, CYP6AB327, increased Taken together, these although accompanied costs, indiscriminate use might elevate level. Overall, findings provide important information about trade-off cost be crucial designing management strategies this key herbivore.
Language: Английский
Citations
1
PLoS ONE, Journal Year: 2024, Volume and Issue: 19(5), P. e0304037 - e0304037
Published: May 24, 2024
Spinosads are insecticides used to control insect pests, especially in organic farming where limited tools for pest management exist. However, resistance has developed spinosads economically important including Colorado potato beetle (CPB), Leptinotarsa decemlineata . In this study, we bioassays determine spinosad sensitivity of two field populations CPB, one from an farm exposed exclusively and a conventional variety insecticides, reference insecticide naïve population. We found the exhibited significant levels compared with sensitive Then, transcriptome profiles between identify genes associated primarily cytochrome P450, CYP9E2 , long non-coding RNA gene, lncRNA-2 were upregulated spinosad-exposed Knock-down these simultaneously beetles population using interference (RNAi) resulted increase mortality when gene knock-down was followed by exposure, whereas single knock-downs each produced smaller effects. addition, individually reduction transcripts. Finally, silico analysis RNA-RNA interaction tool revealed that mRNA contains multiple binding sites transcript. Our results imply jointly contribute is involved regulation expression. These provide evidence metabolic resistance, driven overexpression CYP lncRNA genes, contributes CPB.
Language: Английский
Citations
4
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(6), P. 5461 - 5461
Published: March 13, 2023
Chilo suppressalis is one of the most damaging rice pests in China’s rice-growing regions. Chemical pesticides are primary method for pest control; excessive use insecticides has resulted pesticide resistance. C. highly susceptible to cyproflanilide, a novel with high efficacy. However, acute toxicity and detoxification mechanisms remain unclear. We carried out bioassay experiment larvae found that LD10, LD30 LD50 cyproflanilide 3rd instar was 1.7 ng/per larvae, 6.62 16.92 respectively. Moreover, our field trial results showed had 91.24% control efficiency against suppressalis. investigated effect (LD30) treatment on transcriptome profiles 483 genes were up-regulated 305 down-regulated response exposure, significantly higher CYP4G90 CYP4AU10 expression group. The RNA interference knockdown increased mortality by 20% 18%, respectively, compared control. Our indicate effective insecticidal toxicological activity, involved metabolism. These findings provide an insight into basis means develop efficient resistance management tools
Language: Английский
Citations
7
Pest Management Science, Journal Year: 2024, Volume and Issue: 80(6), P. 2965 - 2975
Published: Feb. 1, 2024
Integrated Pest Management (IPM) seeks to combine multiple management strategies for optimal pest control. One method that is successfully employed in IPM the use of beneficial organisms. However, severe circumstances when insects exceed threshold limits, insecticides may still need be implemented. Thus, understanding effects on biocontrol agents, such as parasitoid wasps, paramount ensure sustainable agroecosystems. Sublethal bioinsecticide spinosyn, a mixture bacterial Saccharopolyspora spinosa (Mertz and Yao) fermentation products spinosyn A D, eggs Trichoplusia ni (Hübner), cruciferous crop pest, its egg Trichogramma brassicae (Bezdenko) was investigated.
Language: Английский
Citations
2
Insects, Journal Year: 2023, Volume and Issue: 14(5), P. 418 - 418
Published: April 27, 2023
The Colorado potato beetle,
Language: Английский
Citations
4