Nature Reviews Rheumatology, Journal Year: 2023, Volume and Issue: 19(11), P. 677 - 677
Published: Oct. 3, 2023
Language: Английский
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: June 19, 2024
Fibroblast growth factors (FGFs) are a versatile family of peptide that involved in various biological functions, including cell and differentiation, embryonic development, angiogenesis, metabolism. Abnormal FGF/FGF receptor (FGFR) signaling has been implicated the pathogenesis multiple diseases such as cancer, metabolic diseases, inflammatory diseases. It is worth noting macrophage polarization, which involves distinct functional phenotypes, plays crucial role tissue repair, homeostasis maintenance, immune responses. Recent evidence suggests FGF/FGFR closely participates polarization macrophages, indicating they could be potential targets for therapeutic manipulation associated with dysfunctional macrophages. In this article, we provide an overview structure, function, downstream regulatory pathways FGFs, well crosstalk between FGF polarization. Additionally, summarize application harnessing to modulate
Language: Английский
Citations
12
Military Medical Research, Journal Year: 2024, Volume and Issue: 11(1)
Published: May 30, 2024
Abstract Orthopedic conditions have emerged as global health concerns, impacting approximately 1.7 billion individuals worldwide. However, the limited understanding of underlying pathological processes at cellular and molecular level has hindered development comprehensive treatment options for these disorders. The advent single-cell RNA sequencing (scRNA-seq) technology revolutionized biomedical research by enabling detailed examination diversity. Nevertheless, investigating mechanisms in highly mineralized skeletal tissue poses technical challenges. In this review, we present a streamlined approach to obtaining high-quality single cells from provide an overview existing scRNA-seq technologies employed studies along with practical bioinformatic analysis pipelines. By utilizing methodologies, crucial insights into developmental dynamics, maintenance homeostasis, involved spine, joint, bone, muscle, tendon disorders been uncovered. Specifically focusing on joint diseases degenerative disc disease, osteoarthritis, rheumatoid arthritis using provided novel more nuanced comprehension. These findings paved way discovering therapeutic targets that offer potential benefits patients suffering diverse
Language: Английский
Citations
9
Nature reviews. Immunology, Journal Year: 2024, Volume and Issue: 24(11), P. 781 - 797
Published: June 24, 2024
Language: Английский
Citations
7
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: Sept. 23, 2024
Rheumatoid arthritis (RA) is an autoimmune disease characterized by synovial inflammation of joints in response to disorders. Once triggered, many factors were involved the development RA, including both cellular like osteoclasts, fibroblasts, T cells, B and soluble interleukin-1 (IL-1), IL-6, IL-17 tumor necrosis factor-α (TNF-α), etc. The complex interplay those results such pathological abnormality as hyperplasia, bone injury multi-joint inflammation. To treat this chronic life-affecting disease, primary drugs used easing patient’s symptoms are disease-modifying antirheumatic (DMARDs). However, these traditional could cause serious side effects, high blood pressure stomach ulcers. Interestingly, recent discoveries on pathogenesis RA have led various new kinds or therapeutic strategies. Therefore, we present a timely review latest field, focusing aspects methods clinical application. Hopefully it can provide translational guide pre-clinical research treatment for joint disease.
Language: Английский
Citations
4
Inflammation and Regeneration, Journal Year: 2025, Volume and Issue: 45(1)
Published: Feb. 20, 2025
Spatial transcriptomics is a cutting-edge technology that analyzes gene expression at the cellular level within tissues while integrating spatial location information. This concept, which combines high-plex RNA sequencing with data, emerged in early 2010s. has rapidly expanded development of technologies such as situ hybridization, sequencing, barcoding, and microdissection-based methods. Each technique offers advanced mapping resolution precise assessments single-cell level. Over past decade, use on clinical samples enabled researchers to identify expressions specific diseased foci, significantly enhancing our understanding interactions disease processes. In field rheumatology, complex elusive pathophysiology diseases rheumatoid arthritis, systemic lupus erythematosus, Sjögren's syndrome remains challenge for personalized treatment. provides insights into how different cell populations interact synovial tissue, kidneys, salivary glands. review summarizes current autoimmune rheumatic diseases, focusing immune distribution tissues. We also explore potential from perspective discuss possibilities translating this bedside.
Language: Английский
Citations
0
Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13
Published: March 26, 2025
Rheumatoid arthritis (RA) is a chronic autoimmune disease that causes synovial joint inflammation as well bone destruction and erosion, typically characterized by pain, swelling, stiffness, with complications persistent pain after remission posing significant health burden for RA patients. The etiology of has not yet been fully elucidated, but large number studies have shown the initiation in closely related to T-cell activation, production variety pro-inflammatory cytokines, macrophage M1/M2 imbalance, homeostatic imbalance intestinal flora, fibroblast-like synoviocytes (FLSs) tissue macrophages (STMs) lumen joints exhibit an aggressive phenotype. While resolution less discussed, therefore, we provided systematic review relevant mechanisms including blocking T cell regulating polarization status, modulating signaling pathway FLSs, subpopulation STMs, inhibiting inflammatory factors, probable its management methods. Achieving crucial improving quality life long-term prognosis Thus, understanding these provide novel potential further drug development treatment RA.
Language: Английский
Citations
0
Connective Tissue Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12
Published: May 10, 2025
This review highlights the transformative impact of spatial transcriptomics on orthopedic research, focusing its application in deciphering intricate gene expression patterns within musculoskeletal tissues. The paper reviews literature for transcriptomic methods, specifically 10X Visium, Xenium, seqFISH+, MERFISH, NanoString GeoMx DSP, used tissues (cartilage, joints, bone, tendon, ligament, and synovium). Searches identified 29 published manuscripts reporting data cartilage, synovium, intervertebral disc. Most publications are from tendon synovium. Visium has been 22 papers identified. Spatial to identify novel cell populations signaling pathways that regulate development disease. Imaging-based methods may be better hypothesis testing as they generally have subcellular resolution but sequence fewer genes. Sequencing untargeted, shotgun approaches can generate useful hypotheses unimpaired tissue sections. could become clinical diagnostics precision medicine approaches.
Language: Английский
Citations
0
Bone Research, Journal Year: 2025, Volume and Issue: 13(1)
Published: May 16, 2025
While bulk RNA sequencing and single-cell have shed light on cellular heterogeneity potential molecular mechanisms in the musculoskeletal system both physiological various pathological states, spatial localization of cells molecules intercellular interactions within tissue context require further elucidation. Spatial transcriptomics has revolutionized biological research by simultaneously capturing gene expression profiles situ information tissues, gradually finding applications research. This review provides a summary recent advances its application to system. The classification characteristics data acquisition techniques are briefly outlined, with an emphasis widely-adopted representative technologies latest technological breakthroughs, accompanied concise workflow for incorporating into role revealing system, particularly during developmental processes, is thoroughly summarized. Furthermore, discoveries achievements this emerging omics tool addressing inflammatory, traumatic, degenerative, tumorous diseases compiled. Finally, challenges future directions transcriptomics, as field discussed.
Language: Английский
Citations
0
Journal of Advanced Research, Journal Year: 2023, Volume and Issue: 61, P. 211 - 221
Published: Sept. 13, 2023
Proprotein convertase subtilisin/kexin type 9 (PCSK9) is implicated in the pathogenesis and progression of autoimmune disease. Patients with rheumatoid arthritis (RA) are at high risk developing atrial fibrillation (AF), while whether PCSK9 involved onset AF among RA patients remains unclear. To explore role occurrence decipher underlying mechanism. We established a rat model collagen-induced (CIA) by immunization II collagen Freund's incomplete adjuvant. Atrial electrophysiological test was used to evaluate susceptibility. performed clinical study examine correlation between level AF, which recruited healthy control, complicated AF. Evolocumab (a monoclonal antibody PCSK9) administered via intraperitoneal injection CIA rats assess RA-related LPS-RS (LPS inhibitor), clodronate liposomes (depletion macrophages), cell co-culture were dissect mechanism promotes inducibility duration higher rats, accompanied elevated plasma PCSK9. Interestingly, compared control subjects, showed an increase PCSK9, much The independently associated patients. In vivo experiment, evolocumab reduced susceptibility, ameliorated structural remodeling rats. Mechanistically, augmented LPS led exacerbated fibrosis cardiac fibroblasts apoptosis myocytes enhancement M1 macrophages polarization inflammation, thereby contributing This suggests that causes atria, can effectively protects from
Language: Английский
Citations
8
Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 1, 2024
Language: Английский
Citations
1