Facile Fluorometric Detection of Faecal Pigments: Challenges and Solutions Concerning Water Quality Testing DOI
Swayam Prakash, Ashok Kumar Mishra

Chemistry - An Asian Journal, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Detection and monitoring of faecal contaminants in water is an important component quality testing protocol worldwide. However, a systematic overview the indicator paradigm, including its fundamentals challenges analytical applications, missing. In particular, with respect to advantages using indication pigments (FIP) over bacteria (FIB). This discussion based on two FIPs, Urobilin (UB) Stercobilin (SB), which can enable rapid real-time ground/surface water. Novel strategies for enhancing sensitive fluorescence-based techniques trace concentration detection have been discussed detail, specific reference understanding their physicochemical properties, photophysics, metal-ligand complexation, molecular aggregations, thermodynamics, fluorescence response matrix interferences aqueous media or environmental samples. The insights provided this perspective article could inspire procedures by avoiding ambiguities misinterpretations.

Language: Английский

A Facile Surface Modification Strategy for Antibody Immobilization on 3D-Printed Surfaces DOI Creative Commons
Brandi Binkley, Peng Li

Biosensors, Journal Year: 2025, Volume and Issue: 15(4), P. 211 - 211

Published: March 25, 2025

3D-printed microdevices have become increasingly important to the advancement of point-of-care (POC) immunoassays. Despite its great potential, using surfaces on solid support for immunorecognition has been limited due non-ideal adsorption properties many photocurable resins. In this work, we report a simple surface modification protocol that works diverse commercial resins, improving ELISAs performed directly devices. This strategy involves activation via air plasma followed by one-step incubation GLYMO-labeled streptavidin. We successfully immobilized biotinylated anti-activin A antibodies and complete ELISA surfaces. demonstrated achieved an improved performance over passive ELISAs. The present method is also compatible with resins both microwells microchannels. Finally, comparable limit detection microwells. believe simplicity broad compatibility will facilitate development POC

Language: Английский

Citations

0
Facile Fluorometric Detection of Faecal Pigments: Challenges and Solutions Concerning Water Quality Testing DOI
Swayam Prakash, Ashok Kumar Mishra

Chemistry - An Asian Journal, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Detection and monitoring of faecal contaminants in water is an important component quality testing protocol worldwide. However, a systematic overview the indicator paradigm, including its fundamentals challenges analytical applications, missing. In particular, with respect to advantages using indication pigments (FIP) over bacteria (FIB). This discussion based on two FIPs, Urobilin (UB) Stercobilin (SB), which can enable rapid real-time ground/surface water. Novel strategies for enhancing sensitive fluorescence-based techniques trace concentration detection have been discussed detail, specific reference understanding their physicochemical properties, photophysics, metal-ligand complexation, molecular aggregations, thermodynamics, fluorescence response matrix interferences aqueous media or environmental samples. The insights provided this perspective article could inspire procedures by avoiding ambiguities misinterpretations.

Language: Английский

Citations

0