Computational Insight in the Identification of Non‐Synonymous Single‐Nucleotide Polymorphism Affecting the Structure and Function of Interleukin‐4 DOI

Pratima Roy,

Siddharth Sharma, Manoj Baranwal

et al.

PROTEOMICS - CLINICAL APPLICATIONS, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

ABSTRACT Background IL4 is a versatile cytokine essentially known for differentiation, proliferation and cell death in cells. Its dysregulation has been found to be associated with the development of inflammatory disorders. Objective The goal current investigation identify select non‐synonymous single‐nucleotide polymorphisms (nsSNPs) IL‐4 gene by employing computational methods which may have potential functional impact on occurrence disease. Method Result Six different nsSNPs were predicted deleterious based consensus algorithms: SIFT, Polyphen2 (Humdiv HumVar), PredictSNP SNP&GO. I‐mutant MuPro assessment revealed decrease stability these mutants except K150M. Modelling was then carried out build wild type along its mutants, followed superimposition evaluate RMSD value, lies between 0.26 0.34. Simulation results mutant models, type, showed that four (N113Y, A118G, R109W K150M) deviated most unstable. A118G significant deviation from while V53A C123R stable. Conclusion finding establishes evidence identified six can new entrant presenting their candidature genetic testing.

Language: Английский

Computational insight in the identification of nonsynonymous single nucleotide polymorphism affecting the structure and function of Interleukin-4 DOI

Pratima Roy,

Siddharth Sharma, Manoj Baranwal

et al.

Authorea (Authorea), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 25, 2024

Background IL4 is a versatile cytokine essentially known for differentiation, proliferation, and cell death in cells. Its dysregulation has been found to be associated with the development of inflammatory disorders. Objective The goal current investigation identify select non-synonymous single nucleotide polymorphisms (nsSNPs) IL-4 gene by employing computational methods which may have potential functional impact on occurrence disease. Method & Result Six different nsSNPs were predicted deleterious based consensus algorithms: SIFT, Polyphen2 (Humdiv HumVar), PredictSNP, SNP&GO.I-mutant MuPro assessment revealed decrease stability these mutants except K150M. Modelling was then carried out build wild-type along its mutants, followed superimposition evaluate RMSD value, lies between 0.26-0.34. Simulation results mutant models, wild-type, showed that four (N113Y, A118G, R109W, K150M) deviated most unstable. A118G significant deviation from while V53A C123R stable. Conclusion finding establishes evidence identified six can new entrant presenting their candidature genetic testing.

Language: Английский

Citations

0
Computational Insight in the Identification of Non‐Synonymous Single‐Nucleotide Polymorphism Affecting the Structure and Function of Interleukin‐4 DOI

Pratima Roy,

Siddharth Sharma, Manoj Baranwal

et al.

PROTEOMICS - CLINICAL APPLICATIONS, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 8, 2024

ABSTRACT Background IL4 is a versatile cytokine essentially known for differentiation, proliferation and cell death in cells. Its dysregulation has been found to be associated with the development of inflammatory disorders. Objective The goal current investigation identify select non‐synonymous single‐nucleotide polymorphisms (nsSNPs) IL‐4 gene by employing computational methods which may have potential functional impact on occurrence disease. Method Result Six different nsSNPs were predicted deleterious based consensus algorithms: SIFT, Polyphen2 (Humdiv HumVar), PredictSNP SNP&GO. I‐mutant MuPro assessment revealed decrease stability these mutants except K150M. Modelling was then carried out build wild type along its mutants, followed superimposition evaluate RMSD value, lies between 0.26 0.34. Simulation results mutant models, type, showed that four (N113Y, A118G, R109W K150M) deviated most unstable. A118G significant deviation from while V53A C123R stable. Conclusion finding establishes evidence identified six can new entrant presenting their candidature genetic testing.

Language: Английский

Citations

0