Nerolidol loaded beta cyclodextrin nanoparticles: a promising strategy for inducing apoptosis in breast cancer cells (MCF-7) DOI
Kamalesh Balakumar Venkatesan,

Saravanan Alamelu,

Manoj Kumar Srinivasan

et al.

Journal of Biomaterials Science Polymer Edition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 31

Published: May 6, 2025

This study investigates the synthesis, characterization and anticancer efficacy of nerolidol-loaded beta cyclodextrin polymeric nanoparticles (NER-βCD-NPs) against MCF-7 breast cancer cells. Nerolidol, a sesquiterpene with anti-inflammatory, antioxidant, antimicrobial properties, faces challenges poor solubility bioavailability, limiting its therapeutic potential. Breast cancer, leading cause cancer-related deaths in women, necessitates alternative therapies fewer side effects compared to conventional chemotherapy. NER-βCD-NPs were synthesized characterized using UV-visible spectroscopy, fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic light scattering (DLS), zeta potential analysis differential calorimetry (DSC). Drug encapsulation efficiency vitro release analyzed HPLC, while molecular docking assessed NER-βCD interactions. Characterization confirmed successful nanoparticle synthesis. spectra FTIR indicated encapsulation-specific changes, SEM revealed surface morphology, DLS, DSC analyses demonstrated increased size stability. The was 84.9%, 86% NER at pH 5.4 over 48 h. Docking studies supported strong binding between βCD (binding energy: -3.55 kcal/mol). Cytotoxicity assays showed significant cell inhibition. Mechanistic reactive oxygen species (ROS) generation, mitochondrial dysfunction, nuclear changes cycle arrest G0-G1 phase. Molecular apoptosis through upregulation Bax, Caspase 6, 9 Cytochrome c, alongside Bcl-2 downregulation. These results highlight as promising strategy for therapy, offering targeted delivery enhanced mitigating nerolidol limitations. Further are warranted validate their clinical applications.

Language: Английский

Nerolidol loaded beta cyclodextrin nanoparticles: a promising strategy for inducing apoptosis in breast cancer cells (MCF-7) DOI
Kamalesh Balakumar Venkatesan,

Saravanan Alamelu,

Manoj Kumar Srinivasan

et al.

Journal of Biomaterials Science Polymer Edition, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 31

Published: May 6, 2025

This study investigates the synthesis, characterization and anticancer efficacy of nerolidol-loaded beta cyclodextrin polymeric nanoparticles (NER-βCD-NPs) against MCF-7 breast cancer cells. Nerolidol, a sesquiterpene with anti-inflammatory, antioxidant, antimicrobial properties, faces challenges poor solubility bioavailability, limiting its therapeutic potential. Breast cancer, leading cause cancer-related deaths in women, necessitates alternative therapies fewer side effects compared to conventional chemotherapy. NER-βCD-NPs were synthesized characterized using UV-visible spectroscopy, fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), dynamic light scattering (DLS), zeta potential analysis differential calorimetry (DSC). Drug encapsulation efficiency vitro release analyzed HPLC, while molecular docking assessed NER-βCD interactions. Characterization confirmed successful nanoparticle synthesis. spectra FTIR indicated encapsulation-specific changes, SEM revealed surface morphology, DLS, DSC analyses demonstrated increased size stability. The was 84.9%, 86% NER at pH 5.4 over 48 h. Docking studies supported strong binding between βCD (binding energy: -3.55 kcal/mol). Cytotoxicity assays showed significant cell inhibition. Mechanistic reactive oxygen species (ROS) generation, mitochondrial dysfunction, nuclear changes cycle arrest G0-G1 phase. Molecular apoptosis through upregulation Bax, Caspase 6, 9 Cytochrome c, alongside Bcl-2 downregulation. These results highlight as promising strategy for therapy, offering targeted delivery enhanced mitigating nerolidol limitations. Further are warranted validate their clinical applications.

Language: Английский

Citations

0