Enzyme and Microbial Technology, Journal Year: 2024, Volume and Issue: 181, P. 110525 - 110525
Published: Oct. 9, 2024
Language: Английский
Enzyme and Microbial Technology, Journal Year: 2024, Volume and Issue: 181, P. 110525 - 110525
Published: Oct. 9, 2024
Language: Английский
Results in Engineering, Journal Year: 2024, Volume and Issue: unknown, P. 103668 - 103668
Published: Dec. 1, 2024
Language: Английский
Citations
10Molecular Catalysis, Journal Year: 2025, Volume and Issue: 573, P. 114814 - 114814
Published: Jan. 5, 2025
Language: Английский
Citations
0Biomass Conversion and Biorefinery, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
Language: Английский
Citations
0Brazilian Journal of Chemical Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: April 12, 2025
Language: Английский
Citations
0Process Biochemistry, Journal Year: 2024, Volume and Issue: 143, P. 117 - 127
Published: April 23, 2024
Language: Английский
Citations
2Bioresource Technology Reports, Journal Year: 2024, Volume and Issue: unknown, P. 101993 - 101993
Published: Nov. 1, 2024
Language: Английский
Citations
2Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 222, P. 120014 - 120014
Published: Nov. 16, 2024
Language: Английский
Citations
1Separation and Purification Technology, Journal Year: 2024, Volume and Issue: 353, P. 128508 - 128508
Published: June 26, 2024
In this work, the salting-out strength of reline eutectic mixture in aqueous solution non-ionic surfactant Triton X-102 has been demonstrated at temperatures between 298.15 and 323.15 K as a preliminary step to be employed downstream operations biotechnological process. The phase diagrams data reline-based Aqueous Two Phase Systems (ATPS) have correlated using empirical models based on exponential polynomial equations. tie-lines empirically ascertained through physical properties characterization Othmer-Tobias equation was proposed correlate those data. After having studied effect two model enzymes (protease lipase), performance ATPS for selectively separating them evaluated three different feed compositions same tie-line, demonstrating their pertinence segregate lipase one phases (yield about 95 %) protease other 98 %). These promising results are interesting application reactions where proteases proved deleterious lipases activity, biocompatibility platform does not involve drastic negative effects any enzymes.
Language: Английский
Citations
0Journal of Biological Engineering, Journal Year: 2024, Volume and Issue: 18(1)
Published: Sept. 2, 2024
Effective enzyme stabilization through immobilization is essential for the functional usage of enzymatic reactions. We propose a new method synthesizing elastic hydroxyapatite microgel (E-HAp-M) materials and immobilizing lipase using this mesoporous mineral via ship-in-a-bottle-neck strategy. The physicochemical parameters E-HAp-M were thoroughly studied, revealing that provides efficient space immobilization. As model enzyme, (LP) was entrapped then cross-linked structure, preventing leaching from mesopores, resulting in highly active stable LP/E-HAp-M composites. By comparing LP activity under different temperature pH conditions, it observed exhibited improved thermal stability resistance compared to free enzyme. In addition, they demonstrated 156% increase catalytic with hydrolysis reactions at room temperature. immobilized maintained 45% its initial after 10 cycles recycling remained over 160 days. This report presents first demonstration stabilized E-HAp-M, suggesting potential application enzyme-catalyzed processes within biocatalysis technology.
Language: Английский
Citations
0Journal of Biotechnology, Journal Year: 2024, Volume and Issue: 395, P. 100 - 109
Published: Sept. 25, 2024
Language: Английский
Citations
0