Mechanisms of Systemic Osmoregulation in Insects

Annual Review of Entomology, Journal Year: 2023, Volume and Issue: 69(1), P. 415 - 438

Published: Sept. 27, 2023

Water is essential to life. Terrestrial insects lose water by evaporation from the body surface and respiratory surfaces, as well in excretory products, posing a challenge made more acute their high surface-to-volume ratio. These losses must be kept minimum offset gained other sources. By contrast, such blood-sucking bug Rhodnius prolixus consume up 10 times weight single blood meal, necessitating rapid expulsion of excess ions. How do manage ion budgets? A century study has revealed great deal about organ systems that use maintain balance regulation. Traditionally, taxonomically wide range species were studied, whereas recent research focused on model organisms leverage power molecular genetic approach. Key advances new technologies have become available for wider past decade. We document how these approaches already begun inform our understanding diversity conservation insect systemic osmoregulation. advocate combined with traditional broader nonmodel gain comprehensive overview mechanism underpinning osmoregulation most species-rich group animals earth, insects.

Language: Английский

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BeetleAtlas: An Ontogenetic and Tissue-specific Transcriptomic Atlas of the Red Flour Beetle Tribolium castaneum

Journal of Molecular Biology, Journal Year: 2024, Volume and Issue: 436(17), P. 168520 - 168520

Published: March 6, 2024

The red flour beetle Tribolium castaneum has emerged as a powerful model in insect functional genomics. However, major limitation the field is lack of detailed spatio-temporal view genetic signatures underpinning function distinct tissues and life stages. Here, we present an ontogenetic tissue-specific web-based resource for transcriptomics — BeetleAtlas (https://www.beetleatlas.org). This web application provides access to database populated with quantitative expression data nine adult seven larval tissues, well four embryonic stages Tribolium. allows one search individual genes obtain values both total gene enrichment different together isoforms. To facilitate cross-species studies, can also use Drosophila melanogaster identifiers related genes. For retrieved there are options identify display tissue or homologous Five additional modes available find conforming any following criteria: exhibiting high particular tissue; showing significant differences between larva adult; having peak at specific stage development; belonging category; displaying pattern similar that query gene. We illustrate how feaures be used illuminate our understanding mechanisms biology what largest animal group on earth.

Language: Английский

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CRF Peptides in Non-mammals

Published: Jan. 1, 2025

Language: Английский

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CAPA Neuropeptide and Its Receptor in Insects: A Mini Review

Archives of Insect Biochemistry and Physiology, Journal Year: 2025, Volume and Issue: 118(5)

Published: April 30, 2025

ABSTRACT A neuropeptide, the CAPA, and its cognate receptor have been diversely characterized in different orders of class Insecta. CAPA peptides are synthesized abdominal neurohemal system activate their corresponding receptor, (CAPA‐R), a type G protein‐coupled (GPCR), to initiate cellular signals for diverse physiological functions insects. Activation CAPA‐R Malpighian tubules results ion‐water homeostasis via antidiuresis majority insect species; however, diuresis myotropic activities also known result. Antidiuretic activity has reported from mosquitoes, assassin bugs, spotted wing drosophila, more; hence, this group holds importance as potential targets when it comes medical agricultural entomology. GPCRs form family cell membrane receptors responsible signal transduction across humans well With advances knowledge human GPCRs, agriculturally important insects offered an opportunity designing implementing GPCR‐targeting compounds integrated pest management programs. In review, we present comprehensive view on factors diuresis/anti‐diuresis with special reference peptide‐receptor interaction. The major focus is role fluid energy homeostasis, stress tolerance, muscle functioning, regulation reproduction, diapause‐related processes. We end by outlining significance excretion respect capa‐r gene silencing management.

Language: Английский

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What Drosophila can tell us about state-dependent peptidergic signaling in insects

Insect Biochemistry and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 104275 - 104275

Published: Feb. 1, 2025

Language: Английский

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RNAi in the Red Flour Beetle (Tribolium Castaneum): Current Status and Future Perspectives

Published: Jan. 1, 2025

Language: Английский

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Crustacean endocrinology

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

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Anti-diuretic hormone ITP signals via a guanylate cyclase receptor to modulate systemic homeostasis inDrosophila

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 10, 2024

Insects have evolved a variety of neurohormones that enable them to maintain their nutrient and osmotic homeostasis. While the identities functions various insect metabolic diuretic hormones been well-established, characterization an anti-diuretic signaling system is conserved across most insects still lacking. To address this, here we characterized ion transport peptide (ITP) in Drosophila . The ITP gene encodes five transcript variants which generate three different isoforms: amidated (ITPa) two ITP-like (ITPL1 ITPL2) isoforms. Using combination anatomical mapping single-cell transcriptome analyses, comprehensively expression all isoforms nervous peripheral tissues. Our analyses reveal widespread Moreover, show ITPa released during dehydration recombinant inhibits peptide-induced renal tubule secretion ex vivo , thus confirming its role as hormone. phylogenetic-driven approach assay, identified functionally Gyc76C, membrane guanylate cyclase, elusive receptor. Thus, knockdown Gyc76C tubules abolishes inhibitory effect on hormone secretion. Extensive reveals it highly expressed larval adult tissues associated with osmoregulation (renal rectum) homeostasis (fat body). Consistent this expression, impacts tolerance ionic stresses, whereas specifically fat body feeding, behaviors. We also complement receptor experiments overexpression neurons. Interestingly, ITPa-Gyc76C pathways deciphered are reminiscent atrial natriuretic mammals. Lastly, utilized connectomics transcriptomics identify synaptic paracrine upstream downstream ITP-expressing Taken together, our systematic establishes tractable decipher how small set neurons integrates diverse inputs orchestrate systemic

Language: Английский

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Allatotropin, DH31, and Proctolin reduce chill tolerance in the two-spotted cricket, Gryllus bimaculatus

Insect Biochemistry and Molecular Biology, Journal Year: 2024, Volume and Issue: unknown, P. 104222 - 104222

Published: Nov. 1, 2024

Language: Английский

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