Anti-inflammatory activities of phycoerythrin and phycocyanin on human fibroblast cells

Phytomedicine Plus, Journal Year: 2024, Volume and Issue: 4(3), P. 100604 - 100604

Published: June 28, 2024

Cyanobacteria are highly regarded for their nutritional value and the scientific community has shown great interest in studying biological activity bioactive compounds, especially pigments. They have a wide range of properties, making them potentially useful various health applications, including dermatology. Given growing demand natural products actives cosmetics medical sectors, these microorganisms emerged as valuable source. Therefore, this study investigated bioactivity phycoerythrin (PE) phycocyanin (PC) extracted from Nostoc sp. Spirulina on CCD-966SK cell lines, specifically focusing apoptosis, necrosis, anti-inflammatory effects, enzymatic reactions. The involved conducting bioassays human skin fibroblast line (CCD-966SK) after performing cyanobacterial culture, pigment extraction, culture. included MTT test, TNF-α IL-6 release assays, measurement collagen I III, assessment apoptosis analysis enzyme superoxide dismutase (SOD) glutathione peroxidase (GPx). cytotoxicity test results indicated that higher concentrations PE PC were associated with lower survival rates. flow cytometry confirmed significant increase necrotic cells decrease living at high both largest percentages identified 125 62.5 ppm, whereas lowest quantities reported 1000 ppm had highest II 3.91 15.63 respectively. levels ppm. released TNF-a cytokines, indicating inflammation resistance. Superoxide (GPx) malondialdehyde (MDA) showed pigments most active In conclusion, exhibited positive effects enhancing necrotic, anti-inflammatory, against line.

Language: Английский

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Storage Properties of Double-Layer Films Enriched with Phytolacca americana L. Extract as Active Packaging for African Catfish, with a New Approach to Antioxidant Film Assay and Additional Analysis of P. americana Extract Toxicity on Human Cell Lines

Molecules, Journal Year: 2025, Volume and Issue: 30(7), P. 1447 - 1447

Published: March 25, 2025

Novel double-layer films based on furcellaran (FUR) and gelatin (GEL) with the addition of Phytolacca americana L. (PA) extract were used as active packaging for African catfish fillets. Films PA have been shown to minimize spoilage effects, expressed odor reduction compared control samples; however, neither nor exhibited antimicrobial activity against tested groups microorganisms (fungi, lactic acid bacteria, Enterobacterales psychrotrops) or specified (E. coli, S. aureus, cerevisiae). The demonstrated antioxidant determined by DPPH, ABTS, FRAP, CUPRAC Folin–Ciocâlteu methods. Cytotoxicity analysis showed that affected cell lines (PNT2—prostate epithelial cells, HepG2—human liver HaCaT—normal human keratinocytes Nty-hori 3-1) only a small extent—the calculated IC50 values exceeded maximal concentration 500 µg/mL.

Language: Английский

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Anti-inflammatory, cytotoxic and morphological impact of phycocyanin on ultraviolet radiation irradiated human fibroblast cells

Cutaneous and Ocular Toxicology, Journal Year: 2024, Volume and Issue: 43(4), P. 396 - 404

Published: Oct. 1, 2024

Recently, the use of natural products as skin photoprotective agents has been in increasing demand. This study investigated bioactivity phycocyanin (PC) extracted from

Language: Английский

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Algae as Crop Plants Being a Source of Bioactive Ingredients of Pharmaceutical and Dietary Importance

Agronomy, Journal Year: 2024, Volume and Issue: 14(5), P. 895 - 895

Published: April 25, 2024

Algae are currently used in many areas, including dietetics, pharmacy, cosmetology and to increase the nutritional value of food animal feed due their chemical composition. They a source extremely valuable molecules, polyunsaturated fatty acids pigments. also protein almost all essential vitamins. rich pigments such as chlorophyll, carotenoids phycobiliproteins. These substances have wide range commercial applications. Due its very intensive use, demand for this plant raw material is constantly growing. Therefore, methods growing harvesting algae improved order maximize cultivation effect while minimizing costs energy inputs. Future research should focus on improving systems, with an emphasis possibility genetic modifications that would allow even more efficient cultivation. This review summarizes micro- macroalgae cultivation, composition selected species, which important from pharmaceutical, dietary cosmetic points view therapeutic applications compounds derived different species. The key conclusion drawn article material, numerous macro- micronutrients necessary humans, acquisition part strategy ecological policy obtaining materials various industries.

Language: Английский

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