WRKY transcription factors in plant defense

Trends in Genetics, Journal Year: 2023, Volume and Issue: 39(10), P. 787 - 801

Published: Aug. 25, 2023

Language: Английский

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A comprehensive review on Gossypium hirsutum resistance against cotton leaf curl virus

Frontiers in Genetics, Journal Year: 2024, Volume and Issue: 15

Published: Feb. 19, 2024

Cotton (

Language: Английский

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Recent advances of CRISPR-based genome editing for enhancing staple crops

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

An increasing population, climate change, and diminishing natural resources present severe threats to global food security, with traditional breeding genetic engineering methods often falling short in addressing these rapidly evolving challenges. CRISPR/Cas systems have emerged as revolutionary tools for precise modifications crops, offering significant advancements resilience, yield, nutritional value, particularly staple crops like rice maize. This review highlights the transformative potential of technology, emphasizing recent innovations such prime base editing, development novel CRISPR-associated proteins, which significantly improved specificity, efficiency, scope genome editing agriculture. These enable targeted that enhance tolerance abiotic stresses well biotic stresses. Additionally, plays a crucial role improving crop yield quality by enhancing photosynthetic nutrient uptake, resistance lodging, while also taste, texture, shelf life, content through biofortification. Despite challenges off-target effects, need more efficient delivery methods, ethical regulatory concerns, underscores importance security sustainability It calls continued research integration CRISPR other emerging technologies nanotechnology, synthetic biology, machine learning fully realize its developing resilient, productive, sustainable agricultural systems.

Language: Английский

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Cotton under heat stress: a comprehensive review of molecular breeding, genomics, and multi-omics strategies

Frontiers in Genetics, Journal Year: 2025, Volume and Issue: 16

Published: March 18, 2025

Cotton is a vital fiber crop for the global textile industry, but rising temperatures due to climate change threaten its growth, quality and yields. Heat stress disrupts key physiological biochemical processes, affecting carbohydrate metabolism, hormone signaling, calcium gene regulation expression. This review article explores cotton's defense mechanism against heat stress, including epigenetic regulations transgenic approaches, with focus on genome editing tools. Given limitations of traditional breeding, advanced omics technologies such as GWAS, transcriptomics, proteomics, ionomics, metabolomics, phenomics CRISPR-Cas9 offer promising solutions developing heat-resistant cotton varieties. highlights need innovative strategies ensure sustainable production under change.

Language: Английский

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Auxin biodynamics and its integral role in enhancing plant resilience to environmental cues

Physiologia Plantarum, Journal Year: 2025, Volume and Issue: 177(2)

Published: March 1, 2025

Auxins are essential plant hormones that regulate growth, development, and responses to environmental stressors. Plants frequently encounter challenges such as pests, diseases, high temperatures, drought, salinity, which necessitate adaptive mechanisms for survival. modulate stress-responsive signaling pathways by regulating gene expression interacting with other phytohormones, thereby influencing physiological processes maintain homeostasis under stress conditions. This review elucidates the molecular through auxins mediate biotic abiotic stresses. The findings indicate pivotal in activating defense pathways. Differential of auxin-related genes has been observed various crops conditions, underscoring their role enhancing resistance against pathogens improving drought tolerance. Additionally, influence root architecture growth responses, facilitating adaptations trichome development herbivory. Moreover, interplay between auxin phytohormones is crucial effective responses. Overall, play a multifaceted enabling plants cope stresses mechanisms. Understanding these complex involving can inform future research aimed at engineering resilient varieties capable thriving changing climates. Further studies needed clarify specific functions contexts develop practical applications crop improvement.

Language: Английский

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Diagnosis of Infectious Diseases by CRISPR/Cas System

OBM Genetics, Journal Year: 2025, Volume and Issue: 09(02), P. 1 - 39

Published: April 1, 2025

Since the initial discovery of CRISPR system in bacteria as an adaptive immune system, a deeper understanding structure and function has made it possible to perform gene editing, therapy, revolutionize diagnostic field. One exciting applications CRISPR-Cas is used tool for rapid diagnosis infectious diseases their treatment prevention infection spread among people. The CRISPR-based could be next-generation standard because its programmability capability searching target sequence quickly, making diagnose infections at lower cost but with same accuracy conventional methods. In this review, data were collected from valid papers published PubMed/Medline, Google Scholar, GISAID, Wiley Online Library, Web Science, ResearchGate databases. We have discussed novel various that routinely soon.

Language: Английский

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Transitioning from wet lab to artificial intelligence: a systematic review of AI predictors in CRISPR

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Feb. 4, 2025

Abstract The revolutionary CRISPR-Cas9 system leverages a programmable guide RNA (gRNA) and Cas9 proteins to precisely cleave problematic regions within DNA sequences. This groundbreaking technology holds immense potential for the development of targeted therapies wide range diseases, including cancers, genetic disorders, hereditary diseases. based genome editing is multi-step process such as designing precise gRNA, selecting appropriate Cas protein, thoroughly evaluating both on-target off-target activity Cas9-gRNA complex. To ensure accuracy effectiveness system, after cleavage, requires careful analysis resultant outcomes indels deletions. Following success artificial intelligence (AI) in various fields, researchers are now leveraging AI algorithms catalyze optimize system. achieve this goal AI-driven applications being integrated into each step, but existing predictors have limited performance many steps still rely on expensive time-consuming wet-lab experiments. primary reason behind low gap between CRISPR fields. Effective integration demands comprehensive knowledge domains. paper bridges research. It offers unique platform grasp deep understanding biological foundations step process. Furthermore, it provides details 80 available system-related datasets that can be utilized develop applications. Within landscape process, insights representation learning methods, machine methods trends, values 50 predictive pipelines. In context classifiers/regressors, thorough pipelines recommendations more robust

Language: Английский

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The importance of genotyping within the climate-smart plant breeding value chain – integrative tools for genetic enhancement programs

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 6, 2025

The challenges faced by today's agronomists, plant breeders, and their managers encompass adapting sustainably to climate variability while working with limited budgets. Besides, are dealing a multitude of issues different organizations on similar initiatives projects, leading lack sustainable impact smallholder farmers. To transform the current food systems as more resilient model efficient solutions needed deliver convey results. Challenges such logistics, labour, infrastructure, equity, must be addressed alongside increasingly unstable conditions which affect life cycle transboundary pathogens pests. In this context, transforming go far beyond just farmers breeders it requires substantial contributions from industry, global finances, transportation, energy, education, country developmental sectors including legislators. As result, holistic approach is essential for achieving sustain population anticipated reach 9.7 billion 2050 11.2 2100. 2021, nearly 193 million individuals were affected insecurity, 40 than in 2020. Meanwhile, digital world rapidly advancing economy estimated at about 20% gross domestic product, suggesting that technologies accessible even areas insecurity. Leveraging these can facilitate development climate-smart cultivars adapt effectively variation, meet consumer preferences, address human livestock nutritional needs. Most economically important traits crops controlled multiple loci often recessive alleles. Considering particularly Africa, continent has several agro-climatic zones, hence need adapted these. Therefore, targeting specific using modern tools offers precise approach. This review article aims how new provide better support

Language: Английский

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Advancing Precision Medicine: Recent Innovations in Gene Editing Technologies

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 2, 2025

The advent of gene editing has significantly advanced the field medicine, opening new frontiers in treatment genetic disorders, cancer, and infectious diseases. Gene technology remains a dynamic promising area research development. Recent advancements protein RNA engineering within this have addressed critical issues such as imprecise edits, poor efficiency, off-target effects. Advancements delivery methods allowed achievement therapeutic or even selection-free efficiency with reduced toxicity primary cells, thereby enhancing safety efficacy manipulation. This progress paves way for transformative changes molecular biology, other fields. review provides comprehensive overview techniques, focusing on prime editor proteins their engineered variants. It also explores alternative systems that expand toolkit precise genomic modifications highlights potential these innovations treating hematological while discussing limitations challenges remain.

Language: Английский

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Genome Editing: An Approach to Engineer Crops to Withstand Climate Change and Sustain Food Security

Published: Jan. 1, 2025

Language: Английский

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