Cooked Bean (Phaseolus vulgaris L.) Consumption Alters Bile Acid Metabolism in a Mouse Model of Diet-Induced Metabolic Dysfunction: Proof-of-Concept Investigation DOI Open Access
Tymofiy Lutsiv,

Vanessa K. Fitzgerald,

Elizabeth S. Neil

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(11), P. 1827 - 1827

Published: May 28, 2025

Background/Objectives: Metabolic dysregulation underlies a myriad of chronic diseases, including metabolic dysfunction-associated steatotic liver disease (MASLD) and obesity, bile acids emerge as an important mediator in their etiology. Weight control by improving diet quality is the standard care prevention these diseases. Inclusion pulses, such common bean, affordable yet neglected approach to outcomes. Thus, this study evaluated possibility that bean alters acid metabolism health-beneficial manner. Methods: Using biospecimens from several similarly designed studies, cecal content, feces, tissue, plasma samples C57BL/6 mice fed obesogenic lacking (control) or containing cooked were subjected total analysis untargeted metabolomics. RNA-seq, qPCR, Western blot assays tissue complemented analyses. Microbial composition predicted function contents using 16S rRNA gene amplicon shotgun metagenomic sequencing. Results: Bean-fed had increased content excreted more per gram feces. Consistent with effects, synthesis was observed. capacity metabolize markedly altered greater prominence secondary metabolites bean-fed mice, i.e., microbial chenodeoxycholate/lithocholate while hyocholate reduced. Conclusions: In rendering resistant diet-induced MASLD consumption sequesters acids, increasing hepatic enhancing diversity through metabolism. Bean-induced changes have potential improve dyslipidemia.

Language: Английский

Arg128*‐Mediated Dual‐Substrate Recognition and Dynamic Transport Mechanisms in (R)‐ω‐Transaminase: Computational Insights and Mutational Profiling Guided Rational Engineering DOI
Jie Chen, Shuai Qiu,

Cong-Lin Ju

et al.

Biotechnology Journal, Journal Year: 2025, Volume and Issue: 20(5)

Published: May 1, 2025

ABSTRACT ω‐Transaminases (ω‐TAs) are critical biocatalysts for the asymmetric synthesis of chiral amines, and uniquely accommodate both hydrophobic hydrophilic substrates through a conserved binding pocket. In this study, we combine computational simulations site‐directed mutagenesis to dissect dual‐function structure ( R )‐selective ω‐transaminase from Aspergillus terreus At ATA). Our results reveal that ATA employs synergistic mechanism: aromatic residues within large pocket stabilize via π‐driven interactions, while Arg128* dynamically interacts with compounds hydrogen bonding. Furthermore, exhibits remarkable plasticity diverse substrates, side chain adjusting its conformation facilitate transport substrates. Mutational profiling, particularly R128*A mutation, directly validates these mechanistic insights. finding reveals Arg128*‐mediated dual‐substrate recognition mechanisms, providing solid theoretical foundation enhancing industrial application transaminases in pharmaceutical green chemistry.

Language: Английский

Citations

0
Cooked Bean (Phaseolus vulgaris L.) Consumption Alters Bile Acid Metabolism in a Mouse Model of Diet-Induced Metabolic Dysfunction: Proof-of-Concept Investigation DOI Open Access
Tymofiy Lutsiv,

Vanessa K. Fitzgerald,

Elizabeth S. Neil

et al.

Nutrients, Journal Year: 2025, Volume and Issue: 17(11), P. 1827 - 1827

Published: May 28, 2025

Background/Objectives: Metabolic dysregulation underlies a myriad of chronic diseases, including metabolic dysfunction-associated steatotic liver disease (MASLD) and obesity, bile acids emerge as an important mediator in their etiology. Weight control by improving diet quality is the standard care prevention these diseases. Inclusion pulses, such common bean, affordable yet neglected approach to outcomes. Thus, this study evaluated possibility that bean alters acid metabolism health-beneficial manner. Methods: Using biospecimens from several similarly designed studies, cecal content, feces, tissue, plasma samples C57BL/6 mice fed obesogenic lacking (control) or containing cooked were subjected total analysis untargeted metabolomics. RNA-seq, qPCR, Western blot assays tissue complemented analyses. Microbial composition predicted function contents using 16S rRNA gene amplicon shotgun metagenomic sequencing. Results: Bean-fed had increased content excreted more per gram feces. Consistent with effects, synthesis was observed. capacity metabolize markedly altered greater prominence secondary metabolites bean-fed mice, i.e., microbial chenodeoxycholate/lithocholate while hyocholate reduced. Conclusions: In rendering resistant diet-induced MASLD consumption sequesters acids, increasing hepatic enhancing diversity through metabolism. Bean-induced changes have potential improve dyslipidemia.

Language: Английский

Citations

0