Biofilm Resilience: Molecular Mechanisms Driving Antibiotic Resistance in Clinical Contexts

Biology, Journal Year: 2025, Volume and Issue: 14(2), P. 165 - 165

Published: Feb. 6, 2025

Healthcare-associated infections pose a significant global health challenge, negatively impacting patient outcomes and burdening healthcare systems. A major contributing factor to healthcare-associated is the formation of biofilms, structured microbial communities encased in self-produced extracellular polymeric substance matrix. Biofilms are critical disease etiology antibiotic resistance, complicating treatment infection control efforts. Their inherent resistance mechanisms enable them withstand therapies, leading recurrent increased morbidity. This review explores development biofilms their dual roles disease. It highlights structural protective functions EPS matrix, which shields populations from immune responses antimicrobial agents. Key molecular biofilm including restricted penetration, persister cell dormancy, genetic adaptations, identified as barriers effective management. implicated various clinical contexts, chronic wounds, medical device-associated infections, oral complications, surgical site infections. prevalence hospital environments exacerbates challenges underscores urgent need for innovative preventive therapeutic strategies. evaluates cutting-edge approaches such DNase-mediated disruption, RNAIII-inhibiting peptides, DNABII proteins, bacteriophage nanoparticle-based solutions, coatings, lock therapies. also examines associated with biofilm-related diagnostic difficulties, disinfectant economic implications. emphasizes multidisciplinary approach importance understanding dynamics, role pathogenesis, advancements strategies combat biofilm-associated effectively settings. These insights aim enhance reduce burden diseases.

Language: Английский

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Advancements and Prospects of Molecularly Imprinted Polymers as Chemical Sensors: A Comprehensive Review

Talanta, Journal Year: 2025, Volume and Issue: 287, P. 127592 - 127592

Published: Jan. 14, 2025

Language: Английский

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Rapid Bacterial/Viral Infections Typing Strategy Using a Portable Dual-Channel Electrochemical Biosensor Based on One-Step Assembly of Immunomagnetic Beads

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Amidst multiple epidemics, a rapid, sensitive, economical, and portable infection diagnosis strategy is crucial for primary medical care, particularly through the analysis of pathogen sources to determine appropriate antibiotic use. C-reactive protein (CRP) serum amyloid A (SAA) are host-related biomarkers, their combined detection can effectively distinguish between bacterial viral infections, which holds great significance unknown pathogens. In this work, dual-channel electrochemical biosensor based on one-step assembly immunomagnetic beads was proposed on-site plasma CRP SAA, streamlined operation shortened minimum time less than 3 min. The exhibited excellent linearity in 3.125-1250 ng/mL 31.25-1250 with limits 0.91 12 ng/mL, respectively, falling within clinically relevant reference range. Through simulated sample tests, distinguished infection, healthy samples. actual tests demonstrated high correlation comparable value enzyme-linked immunosorbent assay. Overall, showed potential aid enable rapid biomarkers.

Language: Английский

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Recent advances in the design of SERS substrates and sensing systems for (bio)sensing applications: Systems from single cell to single molecule detection

F1000Research, Journal Year: 2025, Volume and Issue: 13, P. 670 - 670

Published: March 18, 2025

The Raman effect originates from spontaneous inelastic scattering of photons by matter. These provide a characteristic fingerprint this matter, and are extensively utilized for chemical biological sensing. inherently lower generation these scattered photons, do not hold potential their direct use in sensing applications. Surface enhanced spectroscopy (SERS) overcomes the low sensitivity associated with assists diverse analytes, including ions, small molecules, inorganics, organics, radionucleotides, cells. Plasmonic nanoparticles exhibit localized surface plasmon resonance (LSPR) when they closely spaced, create hotspots where electromagnetic field is significantly enhanced. This amplifies signal may offer up to 10 14-fold SERS enhancement. development active substrates requires further consideration optimization several critical features such as periodicity, hotspot density, mitigation sample or autofluorescence, tuning hydrophilicities, specific (bio) recognition elements suitable linkers bioconjugation chemistries, appropriate optics obtain relevant outcomes terms sensitivity, cross-sensitivity, limit detection, signal-to-noise ratio (SNR), stability, shelf-life, disposability. article comprehensively reviews recent advancements on disposable materials commercial grades paper, textiles, glasses, polymers, some blue-ray digital versatile discs (DVDs) SERS-active point-of-use (POU) technologies have been reviewed critiqued analyte detection resource-limited settings, highlighting prospects applications ranging single-molecule single-cell detection. We conclude possible avenues developing viable deployable sensors holding immense environmental monitoring, food safety biomedical diagnostics.

Language: Английский

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Advancing gut microbiome insights: Graphene quantum dot nanobiosensors for microbial metabolite detection

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162954 - 162954

Published: April 1, 2025

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