3D Printed Spectroelectrochemical Platform for Redox‐Based Bioelectronics DOI Creative Commons
Chen‐Yu Chen, Eunkyoung Kim, Fauziah Rahma Zakaria

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Redox provides unique opportunities for interconverting molecular/biological information into electronic signals. Here, the fabrication of a 3D‐printed multiwell device that can be interfaced existing laboratory instruments (e.g., well‐plate readers and microscopes) to enable advanced redox‐based spectral electrochemical capabilities is reported. In first application, mediated probing used as soft sensing method biomanufacturing: it shown signal metrics discern intact mAbs from partially reduced mAb variants (fragmentation), these near‐real‐time electrical measurements correlate off‐line chemical analysis. second operando spectroelectrochemical are characterize redox‐active catechol‐based hydrogel film: electron transfer into/from film correlates molecular switching film's redox state with absorbance increasing upon oxidation fluorescence reduction. final example, synthetic biofilm containing redox‐responsive E. coli electro‐assembled: gene expression induced under reducing conditions (via reductive H 2 O generation) or oxidative phenolic redox‐signaling molecule). Overall, this work demonstrates 3D printing allows bespoke devices accelerate understanding phenomena in biology detection/characterization activities technology.

Language: Английский

3D Printed Spectroelectrochemical Platform for Redox‐Based Bioelectronics DOI Creative Commons
Chen‐Yu Chen, Eunkyoung Kim, Fauziah Rahma Zakaria

et al.

Small Methods, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 29, 2025

Abstract Redox provides unique opportunities for interconverting molecular/biological information into electronic signals. Here, the fabrication of a 3D‐printed multiwell device that can be interfaced existing laboratory instruments (e.g., well‐plate readers and microscopes) to enable advanced redox‐based spectral electrochemical capabilities is reported. In first application, mediated probing used as soft sensing method biomanufacturing: it shown signal metrics discern intact mAbs from partially reduced mAb variants (fragmentation), these near‐real‐time electrical measurements correlate off‐line chemical analysis. second operando spectroelectrochemical are characterize redox‐active catechol‐based hydrogel film: electron transfer into/from film correlates molecular switching film's redox state with absorbance increasing upon oxidation fluorescence reduction. final example, synthetic biofilm containing redox‐responsive E. coli electro‐assembled: gene expression induced under reducing conditions (via reductive H 2 O generation) or oxidative phenolic redox‐signaling molecule). Overall, this work demonstrates 3D printing allows bespoke devices accelerate understanding phenomena in biology detection/characterization activities technology.

Language: Английский

Citations

0