Navigating the Purification Process: Maintaining the Integrity of Replication-Competent Enveloped Viruses DOI Creative Commons
Adrian Schimek, Judy King Man Ng, Jürgen Hubbuch

et al.

Vaccines, Journal Year: 2025, Volume and Issue: 13(5), P. 444 - 444

Published: April 23, 2025

Replication-competent virus particles hold significant therapeutic potential in application as oncolytic viruses or cancer vaccines. Ensuring the viral integrity of these is crucial for their infectivity, safety, and efficacy. Enveloped particles, particular, offer large gene insert capacities customizable target specificity. However, sensitivity to environmental factors presents challenges bioprocessing, potentially compromising high quality standards cost-effective production. This review provides an in-depth analysis purification process steps replication-competent enveloped emphasizing importance maintaining integrity. It evaluates bioprocessing methods from cell culture harvest final sterile filtration, including centrifugation, chromatographic, filtration techniques. Furthermore, manuscript delves into formulation storage strategies necessary preserve functional structural ensuring long-term stability To assess impact on determine integrity, advanced analytical are required. commonly used assessing such infectious titer assays, total particle quantification, analysis. By providing a comprehensive overview current state this aims guide researchers industry professionals developing robust efficient processes. The insights gained will contribute advancement virus-based therapeutics, ultimately supporting development safe, effective, economically viable treatments various diseases.

Language: Английский

Navigating the Purification Process: Maintaining the Integrity of Replication-Competent Enveloped Viruses DOI Creative Commons
Adrian Schimek, Judy King Man Ng, Jürgen Hubbuch

et al.

Vaccines, Journal Year: 2025, Volume and Issue: 13(5), P. 444 - 444

Published: April 23, 2025

Replication-competent virus particles hold significant therapeutic potential in application as oncolytic viruses or cancer vaccines. Ensuring the viral integrity of these is crucial for their infectivity, safety, and efficacy. Enveloped particles, particular, offer large gene insert capacities customizable target specificity. However, sensitivity to environmental factors presents challenges bioprocessing, potentially compromising high quality standards cost-effective production. This review provides an in-depth analysis purification process steps replication-competent enveloped emphasizing importance maintaining integrity. It evaluates bioprocessing methods from cell culture harvest final sterile filtration, including centrifugation, chromatographic, filtration techniques. Furthermore, manuscript delves into formulation storage strategies necessary preserve functional structural ensuring long-term stability To assess impact on determine integrity, advanced analytical are required. commonly used assessing such infectious titer assays, total particle quantification, analysis. By providing a comprehensive overview current state this aims guide researchers industry professionals developing robust efficient processes. The insights gained will contribute advancement virus-based therapeutics, ultimately supporting development safe, effective, economically viable treatments various diseases.

Language: Английский

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