Metabolite Identification of Huangqi Guizhi Wuwu Granules in Rat Urine and Feces Based on Ultra–High‐Performance Liquid Chromatography With Quadrupole Time‐of‐Flight Mass Spectrometry DOI Open Access
Yang Liu, Yang Yan, Wang Xing

et al.

Biomedical Chromatography, Journal Year: 2025, Volume and Issue: 39(5)

Published: March 17, 2025

ABSTRACT Huangqi Guizhi Wuwu Decoction (HGWD), a famous Chinese medicine prescription, has been widely used in the treatment of diabetic peripheral neuropathy and scleroderma China. Considering that decoction was not easy to store carry, our research group converted HGWD into Granules (HGWG) early stage. However, vivo metabolism profile HGWG still unclear. In this study, an ultra–high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (UHPLC‐Q‐TOF‐MS) method established identify prototypes metabolites urine feces rats after oral administration HGWG. A total 100 compounds were identified samples. samples, 28 prototype 41 related identified. 39 12 Monoterpenoid glucosides, flavonoids, organic acids, gingerols, terpenes main both rat feces. Flavonoid‐related metabolites, acid‐related gingerol‐related major urine, flavonoid‐related This study can provide theoretical basis for drug new development

Language: Английский

Metabolite Identification of Huangqi Guizhi Wuwu Granules in Rat Urine and Feces Based on Ultra–High‐Performance Liquid Chromatography With Quadrupole Time‐of‐Flight Mass Spectrometry DOI Open Access
Yang Liu, Yang Yan, Wang Xing

et al.

Biomedical Chromatography, Journal Year: 2025, Volume and Issue: 39(5)

Published: March 17, 2025

ABSTRACT Huangqi Guizhi Wuwu Decoction (HGWD), a famous Chinese medicine prescription, has been widely used in the treatment of diabetic peripheral neuropathy and scleroderma China. Considering that decoction was not easy to store carry, our research group converted HGWD into Granules (HGWG) early stage. However, vivo metabolism profile HGWG still unclear. In this study, an ultra–high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (UHPLC‐Q‐TOF‐MS) method established identify prototypes metabolites urine feces rats after oral administration HGWG. A total 100 compounds were identified samples. samples, 28 prototype 41 related identified. 39 12 Monoterpenoid glucosides, flavonoids, organic acids, gingerols, terpenes main both rat feces. Flavonoid‐related metabolites, acid‐related gingerol‐related major urine, flavonoid‐related This study can provide theoretical basis for drug new development

Language: Английский

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