Journal of Clinical Medicine,
Journal Year:
2024,
Volume and Issue:
13(23), P. 7176 - 7176
Published: Nov. 26, 2024
Undifferentiated
pleomorphic
sarcoma
(UPS)
is
a
highly
malignant
mesenchymal
tumor
that
ranks
as
one
of
the
most
common
types
soft
tissue
sarcoma.
Even
though
chemotherapy
increases
5-year
survival
rate
in
UPS,
high
heterogeneity
frequently
leads
to
resistance
and
consequently
recurrences.
In
this
study,
we
characterized
cell
composition
transcriptional
profile
UPS
with
at
single
resolution.
PLoS ONE,
Journal Year:
2024,
Volume and Issue:
19(7), P. e0307776 - e0307776
Published: July 26, 2024
Deubiquitinating
enzymes
(DUBs)
play
a
pivotal
role
in
regulating
the
antiviral
immune
response
by
targeting
members
of
RLR
signaling
pathway.
As
member
pathway,
TRAF3
is
essential
for
activating
MAVS/TBK-1/IRF3
pathway
to
viral
infection.
Despite
its
importance,
function
DUBs
TRAF3-mediated
poorly
understood.
Ubiquitin-specific
protease
26
(USP26)
regulates
modulate
response.
The
results
demonstrate
that
EV71
infection
upregulates
expression
USP26.
Knockdown
USP26
significantly
enhances
EV71-induced
IFN-β
and
downstream
interferon-stimulated
genes
(ISGs).
Deficiency
not
only
inhibits
replication
but
also
weakens
host's
resistance
physically
interacts
with
reduces
K63-linked
polyubiquitination
TRAF3,
thereby
promoting
pIRF3-mediated
signaling.
Conversely,
knockdown
leads
an
increase
TRAF3.
These
findings
unequivocally
establish
contribute
understanding
deubiquitination-mediated
regulation
innate
responses.
Scientific Reports,
Journal Year:
2024,
Volume and Issue:
14(1)
Published: Aug. 13, 2024
Hepatocellular
carcinoma
(HCC)
still
presents
poor
prognosis
with
low
overall
survival
rates
and
limited
therapeutic
options
available.
Recently,
attention
has
been
drawn
to
peptidomic
analysis,
an
emerging
field
of
proteomics
for
the
exploration
new
potential
peptide
drugs
treatment
various
diseases.
However,
research
on
function
HCC
peptides
is
lacking.
Here,
we
analyzed
spectrum
in
tissues
using
techniques
explored
potentially
beneficial
involved
HCC.
Changes
profiles
were
examined
liquid
chromatography-mass
spectrometry
(LC–MS/MS).
Analyze
physicochemical
properties
differently
expressed
bioinformatics.
The
effect
candidate
functional
cell
growth
migration
was
evaluated
CCK-8,
colony
formation,
transwell
assays.
Transcriptome
sequencing
analysis
western
blot
employed
delve
into
mode
action
Peptidomic
tissue
yielded
a
total
8683
peptides,
which
452
exhibited
up-regulation
362
showed
down-regulation.
that
differentially
expressed,
according
bioinformatic
closely
linked
carbon
metabolism
mitochondrial
inner
membrane.
validation
identified
novel
peptide,
PDLC
(peptide
derived
from
liver
cancer),
found
dramatically
boost
proliferation
through
Ras/Raf/MEK/ERK
signaling
cascade.
Our
defined
peptide's
pattern
expression
PDLC,
encouraging
progression,
offering
entirely
target
disease.
Genes,
Journal Year:
2024,
Volume and Issue:
15(10), P. 1278 - 1278
Published: Sept. 29, 2024
Compound
Ku-Shen
Injection
(CKI)
is
a
traditional
Chinese
medicine
preparation
derived
from
and
Bai-Tu-Ling,
commonly
used
in
the
adjunctive
treatment
of
advanced
cancers,
including
liver
cancer.
However,
underlying
mechanisms
CKI's
effectiveness
cancer
are
not
well
defined.
Diabetes Obesity and Metabolism,
Journal Year:
2024,
Volume and Issue:
27(2), P. 750 - 766
Published: Nov. 18, 2024
Abstract
Background
Diabetic
nephropathy
(DN)
is
one
of
the
main
causes
end‐stage
renal
disease
(ESRD),
but
its
mechanism
has
not
been
clearly
studied.
We
utilized
integrative
transcriptome
analysis
to
explore
pathogenesis
DN.
Methods
conducted
an
by
combining
bulk
dataset
and
single‐cell
dataset.
Through
this
approach,
we
identified
that
Serpine2
may
regulate
‘collagen‐containing
extracellular
matrix’
pathway
involved
in
Subsequently,
established
DN
animal
cell
models
using
db/db
mice
mesangial
cells
(MCs)
validate
role
In
model,
detected
expression
level
western
blotting
(WB)
immunofluorescence
(IF)
assays.
To
further
clarify
molecular
DN,
knocked
down
observed
effects
on
MCs
proliferation
matrix
(ECM)
accumulation.
Results
Our
highlighted
a
pivotal
for
disease's
initiation.
Next,
through
Cytoscape
differentially
expressed
genes
(DEGs)
MCs,
following
10
hub
genes:
Acta2,
Angpt2,
Ccn1,
Col4a1,
Col4a2,
Col8a1,
Kdr,
Thbs1,
Tpm4
Serpine2.
Kdr
were
also
significantly
DEGs
glomeruli.
Additionally,
our
integrated
gene
set
enrichment
RNA
revealed
was
key
progression.
crucial
regulating
pathway.
Therefore,
speculated
regulation
important
mechanism.
Importantly,
WB
IF
staining
confirmed
upregulated
diabetic
mice.
Knockdown
cultured
alleviated
high‐glucose‐induced
excessive
ECM
Finally,
found
ERK
agonist
Ro
67‐7476
eliminated
effect
siRNA.
Conclusions
summary,
regulates
synthesis
activation
ERK1/2
pathway,
which
These
findings
offer
fresh
perspectives
mechanisms
glomerulosclerosis
provide
new
targets
treating
Journal of Clinical Medicine,
Journal Year:
2024,
Volume and Issue:
13(23), P. 7176 - 7176
Published: Nov. 26, 2024
Undifferentiated
pleomorphic
sarcoma
(UPS)
is
a
highly
malignant
mesenchymal
tumor
that
ranks
as
one
of
the
most
common
types
soft
tissue
sarcoma.
Even
though
chemotherapy
increases
5-year
survival
rate
in
UPS,
high
heterogeneity
frequently
leads
to
resistance
and
consequently
recurrences.
In
this
study,
we
characterized
cell
composition
transcriptional
profile
UPS
with
at
single
resolution.
