Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 154, P. 108069 - 108069
Published: Dec. 16, 2024
Language: Английский
Cancer Communications, Journal Year: 2024, Volume and Issue: 44(11), P. 1316 - 1336
Published: Sept. 21, 2024
Abstract Glycosylation, a key mode of protein modification in living organisms, is critical regulating various biological functions by influencing folding, transportation, and localization. Changes glycosylation patterns are significant feature cancer, associated with range pathological activities cancer‐related processes, serve as biomarkers providing new targets for cancer diagnosis treatment. Glycoproteins like human epidermal growth factor receptor 2 (HER2) breast alpha‐fetoprotein (AFP) liver carcinoembryonic antigen (CEA) colon prostate‐specific (PSA) prostate all tumor approved clinical use. Here, we introduce the diversity structures newly discovered substrate—glycosylated RNA (glycoRNA). This article focuses primarily on metastasis, immune evasion, metabolic reprogramming, aberrant ferroptosis responses, cellular senescence to illustrate role cancer. Additionally, summarize applications diagnostics, treatment, multidrug resistance. We envision promising future glycosylation.
Language: Английский
Citations
10
Proteoglycan Research, Journal Year: 2025, Volume and Issue: 3(1)
Published: Jan. 1, 2025
ABSTRACT Alterations in glycoconjugate profiles are thought to promote changes cell‐to‐cell and cell‐to‐intracellular extracellular scaffold interactions human disease. The nearly unlimited number of “glycoforms” that may exist nature difficult study due glycosylation modifications being associated with non‐genome coded posttranscription post‐translation processes. Specific products generated by dependent on concentration sub‐cellular locations glycan synthesis processing enzymes. An indirect “high‐throughput” approach is characterize enzymes (hydrolases transferases) single cell sequencing all types tissue diseases. We previously identified TMEM230 as an endoplasmic reticulum (ER) protein regulates NOTCH glycoprotein receptor ligand signaling zebrafish blood vessel formation destructive remodeling capacities diverse including fibroblast, phagocytic immune system cells patients cancer or granulomatous systemic vasculitis autoimmune disorder. represents a paradigm mediated signal transduction supports the role modifications. ER initiates earliest steps synthesis, sorting, trafficking. As remodeling, Notch hallmarks disorders, we investigated whether aberrant expression was also rheumatoid arthritis (RA). In this current study, analysis supported downregulated synovial RA while were predominantly upregulated. contrast, upregulated high‐grade compared low‐grade gliomas it N‐linked (GlcNAc), glycosaminoglycan expression. Our collective results support glycan/glycoconjugate aggressive gliomas. therefore be therapeutic target marker for clinical treatment induced autoimmunity disorders cancer.
Language: Английский
Citations
1
Engineering, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(6), P. 2412 - 2412
Published: March 7, 2025
Aging is often a choice between developing cancer or autoimmune disorders, due in part to loss of self-tolerance immunological recognition rogue-acting tumor cells. Self-tolerance and cell by the immune system are processes very much dependent on specific signatures glycans glycosylated factors present plasma membrane stromal components tissue. Glycosylated generated nearly innumerable variations nature, allowing for immensely diverse role these aging flexibility necessary cellular interactions tissue functionality. In previous studies, we showed that differential expression TMEM230, an endoplasmic reticulum (ER) protein was associated with enzymes regulating glycan synthesis processing glycosylation rheumatoid arthritis synovial using single-cell transcript sequencing. this current study, characterize genes pathways co-modulated all types processing, as well glycosylation. Genes biological molecular hallmarks were mitochondria-dependent oxidative phosphorylation reactive oxygen species synthesis, ER-dependent stress unfolded response, DNA repair (UV response P53 signaling pathways), senescence, glycolysis apoptosis regulation through PI3K-AKT-mTOR have been shown play important roles neurodegeneration (such Parkinson’s Alzheimer’s disease). We propose downregulation TMEM230 RNASET2 may represent paradigm study age-dependent disorders their glycosylation, signaling.
Language: Английский
Citations
0
Critical Reviews in Oncology/Hematology, Journal Year: 2025, Volume and Issue: 210, P. 104700 - 104700
Published: March 12, 2025
Cancer immunotherapy has become a revolutionary strategy in oncology, utilizing the host immune system to fight malignancies. Notwithstanding major progress, obstacles such as evasion by tumors and development of resistance still remain. This manuscript examines function chaperone-mediated autophagy (CMA) cancer biology, focusing on its effects tumor response resistance. CMA is selective degradation mechanism for cytosolic proteins, which crucial sustaining cellular homeostasis regulating responses. By degrading specific can either facilitate progression stressful conditions, or promote suppression removing oncogenic factors. double-edged sword highlights complexity possible effect treatment results. Here we clarify molecular mechanisms regulate role therapeutic target improving effectiveness immunotherapy.
Language: Английский
Citations
0
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: March 15, 2025
Abstract Extracellular vesicles (EVs) are critical mediators of intercellular communication and hold promise as biomarkers therapeutic targets in cancer, but their molecular alterations remain poorly understood. Protein glycosylation is a frequent post-translational modification; however, most EV studies focus only on proteomics, while mapping changes proteins still underrepresented. To address this shortcoming, we analyzed the proteomic, N -glycoproteomic, chondroitin/dermatan sulfate (CS/DS) glycosaminoglycan (GAG) profiles small EVs (sEVs) derived from A549 lung adenocarcinoma BEAS-2B non-tumorigenic epithelial cell lines. Principal component analysis hierarchical clustering revealed that all three highly dependent origin sEV, highlighting fundamental differences not at proteomic also -glycopeptide CS/DS levels. expression were primarily associated with upregulation cycle regulation, DNA repair, metabolism, protein synthesis, immune-related processes predominantly downregulated. Proteomics differential expressions 5 CS proteoglycans, anticipating profile may change. -glycoproteomics highlighted shift complex to hybrid -glycans cancer sEVs, alongside significant decrease fucosylation. Prominent glycoproteins characterized multiple sites included versican, galectin-3-binding laminins. The total amount increased 3.4-fold ratio two monosulfated disaccharides changed 2-fold, suggesting altered sulfation mechanisms. These findings highlight potential GAG profiling enhance biomarker discovery EV-based diagnostics. Graphical abstract Proteomic, -glycoproteomic disaccharide differ between extracellular vesicles.
Language: Английский
Citations
0
Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162728 - 162728
Published: April 1, 2025
Language: Английский
Citations
0
Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)
Published: April 22, 2025
The global incidence of prostate cancer (PCa) has been rising annually, and early diagnosis treatment remain pivotal for improving therapeutic outcomes patient prognosis. Concurrently, advancements in liquid biopsy technology have facilitated disease monitoring, with its minimally invasive nature low heterogeneity positioning it as a promising approach predicting progression. However, current strategies PCa predominantly rely on prostate-specific antigen (PSA), which lacks specificity compromises diagnostic accuracy. Thus, there is an urgent need to identify novel biomarkers enable precise diagnosis. We integrated 12 machine learning algorithms construct 113 combinatorial models, screening validating optimal panel across five datasets from TCGA GEO databases. Subsequently, the biological feasibility selected predictive model was verified one epithelial cell line lines. Robust RNA targets were further validated their expression plasma samples establish RNA-based strategy PCa. Finally, benign prostatic hyperplasia (BPH) patients at Wuhan Tongji Hospital collected evaluate strategy's clinical significance. Differential analysis identified 1,071 candidate mRNAs, input into framework. Among 9-gene by Stepglm[both] Enet[alpha = 0.4] demonstrated highest efficacy (mean AUC 0.91), including JPH4, RASL12, AOX1, SLC18A2, PDZRN4, P2RY2, B3GNT8, KCNQ5, APOBEC3C. Cell experiments AOX1 B3GNT8 robust biomarkers, both exhibiting consistent PCa-specific human samples. In analyses, outperformed PSA accuracy, achieving combined 0.91. Notably, these also utility ISUP ≤ 2. Through validation, we developed Specifically, value. These findings provide new insights
Language: Английский
Citations
0
Mass Spectrometry Reviews, Journal Year: 2025, Volume and Issue: unknown
Published: April 23, 2025
ABSTRACT Cancer is the leading cause of death worldwide characterized by patient heterogeneity and complex tumor microenvironment. While genomics‐based testing has transformed modern medicine, challenge diverse clinical outcomes highlights unmet needs for precision oncology. As functional molecules regulating cellular processes, proteins hold great promise as biomarkers drug targets. Mass spectrometry (MS)‐based proteomics illuminated molecular features cancers facilitated discovery or therapeutic targets, paving way innovative strategies that enhance personalized treatment. In this article, we introduced tools current achievements MS‐based proteomics, choice targeted MS from to validation phases, profiling sensitivity bulk samples single‐cell level tissue liquid biopsy specimens, regulatory landscape protein laboratory‐developed tests (LDTs). The challenges, success future perspectives in translating research assay into applications are also discussed. With well‐designed studies demonstrate benefits meet requirements both analytical performance, assays promising with numerous opportunities improve cancer diagnosis, treatment, monitoring.
Language: Английский
Citations
0
Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 154, P. 108069 - 108069
Published: Dec. 16, 2024
Language: Английский
Citations
0