Ginsenoside Rk1 induces autophagy-dependent apoptosis in hepatocellular carcinoma by AMPK/mTOR signaling pathway

Food and Chemical Toxicology, Journal Year: 2024, Volume and Issue: 186, P. 114587 - 114587

Published: March 8, 2024

Language: Английский

---

Research progress on rodent models and its mechanisms of liver injury

Life Sciences, Journal Year: 2023, Volume and Issue: 337, P. 122343 - 122343

Published: Dec. 15, 2023

The liver is the most important organ for biological transformation in body and crucial maintaining body's vital activities. Liver injury a serious pathological condition that commonly found many diseases. It has high incidence rate, difficult to cure, prone recurrence. can cause harm body, ranging from mild severe fatty disease. If continues worsen, it lead fibrosis cirrhosis, ultimately resulting failure or cancer, which seriously endanger human life health. Therefore, establishing an rodent model mimics pathogenesis severity of clinical great significance better understanding patients developing more effective treatment methods. author this article summarizes common chemical models, immune alcoholic drug-induced systematically elaborates on modeling methods, mechanisms action, pathways advantages disadvantages each type model. aim study establish reliable models researchers use exploring anti-liver hepatoprotective drugs. By creating accurate theoretical frameworks, we hope provide new insights into

Language: Английский

---

Emerging and potential use of CRISPR in human liver disease

Hepatology, Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 22, 2023

CRISPR is a gene editing tool adapted from naturally occurring defense systems bacteria. It technology that revolutionizing the interrogation of functions in driving liver disease, especially through genetic screens and by facilitating animal knockout knockin models. being used models disease to identify which genes are critical for pathology, hepatitis, cancer initiation progression. holds tremendous promise treating human diseases directly DNA. could disable function case expression maladaptive protein, such as blocking transthyretin therapy amyloidosis, or correct defects, restoring normal enzymes fumarylacetoacetate hydrolase alpha-1 antitrypsin. also studied treatment hepatitis B infection. an exciting, evolving characterization discovery potential treat safely permanently.

Language: Английский

---

Advances in experimental animal models of hepatocellular carcinoma

Cancer Medicine, Journal Year: 2023, Volume and Issue: 12(14), P. 15261 - 15276

Published: May 29, 2023

Hepatocellular carcinoma (HCC) is a common malignant tumor with insidious early symptoms, easy metastasis, postoperative recurrence, poor drug efficacy, and high resistance rate when surgery missed, leading to low 5-year survival rate. Research on the pathogenesis drugs particularly important for clinical treatment. Animal models are crucial basic research, which conducive studying screening more conveniently effectively. An appropriate animal model can better reflect disease occurrence development, process of anti-tumor immune response in human body. This review summarizes classification, characteristics, advances experimental HCC provide reference researchers selection.

Language: Английский

---

FIGNL1 Promotes Hepatocellular Carcinoma Formation via Remodeling ECM-receptor Interaction Pathway Mediated by HMMR

Current Gene Therapy, Journal Year: 2023, Volume and Issue: 24(3), P. 249 - 263

Published: Nov. 6, 2023

Background: The development of novel biomarkers is crucial for the treatment HCC. In this study, we investigated a new molecular therapeutic target Fidgetin-like 1 (FIGNL1) has been reported to play vital role in lung adenocarcinoma. However, potential function FIGNL1 HCC still unknown. Objective: This study aims investigate key regulatory mechanisms formation Methods: effect on was studied by lentivirus infection. vitro, effects proliferation, migration and apoptosis cells were CCK8, colony assay, transwell flow cytometry. Meanwhile, regulation vivo subcutaneous transplanted tumors. addition, using transcriptome sequencing technology, further explored specific mechanism regulating Results: Functionally, demonstrated that knockdown significantly inhibited cell promoted vitro. Similarly, meaningfully weakened hepatocarcinogenesis nude mice. Transcriptome revealed affected expression genes involved extracellular matrix-receptor (ECM-receptor) interaction pathway, such as hyaluronan mediated motility receptor (HMMR). Further validation found overexpression HMMR based can rescue abundance related ECM-receptor pathway. Conclusion: Our could modulate pathway through HMMR, thus

Language: Английский

---

Plasmodium infection downregulates hypoxia‑inducible factor 1α expression to suppress the vascularization and tumorigenesis of liver cancer

Oncology Letters, Journal Year: 2024, Volume and Issue: 28(6)

Published: Oct. 14, 2024

Liver cancer is characterized by hypervascularization. Anti‑angiogenic agents may normalize the tumor vasculature and improve efficacy of other treatments. The present study aims to investigate anti‑angiogenic effect Plasmodium infection in a mouse model implanted liver cells. HepG2 cells were injected into left lobe nude mice as in situ hepatic tumorigenesis. Plasmodium yoelii parasitized erythrocytes administered animal introduce infection. growth microvascular density determined presence or absence expression levels hypoxia‑inducible factor 1α (HIF‑1α) angiogenesis‑related factors evaluated using western blotting reverse transcription‑quantitative PCR analysis. results demonstrated that suppressed vascularization parasites reduced pro‑angiogenic (vascular endothelial A angiopoietin 2), matrix metalloproteinases [(MMP)2 MMP9] inflammatory cytokines [tumor necrosis α, interleukin 6 (IL)‑6) IL‑1β] both tissues. HIF‑1α was downregulated tissues upon infection, overexpression rescued angiogenesis under condition In conclusion, anti‑tumorigenic effects on through downregulating expression, indicating Plasmodium could be developed an intervention strategy restrain neo‑angiogenesis cancer.

Language: Английский

---

A rat model of cirrhosis with well-differentiated hepatocellular carcinoma induced by thioacetamide

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 22, 2024

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths, and commonly associated with hepatic fibrosis or cirrhosis. This study aims to establish rat model mimicking the progression from liver cirrhosis subsequently HCC using thioacetamide (TAA). We utilized male Lewis rats, treating them intra-peritoneal injections TAA. These rats received bi-weekly either 200 mg/kg TAA saline (as control) over period 34 weeks. The development hepatocarcinogenesis was monitored through histopathological examinations, biochemical markers, immunohistochemical analyses. Our results demonstrated that chronic administration induced well-differentiated HCC, characterized by increased fibrosis, altered architecture, enhanced hepatocyte proliferation. Biochemical analyses revealed significant alterations in function including elevated alpha-fetoprotein (AFP) levels, without affecting kidney causing weight loss mortality rats. TAA-induced successfully replicates clinical human impairment early-stage cancer characteristics. It presents valuable tool for future research on mechanisms antitumor drugs tumor initiation development.

Language: Английский

---

Clinical potential of serum prostaglandin A2 as a novel diagnostic biomarker for hepatocellular cancer

Clinica Chimica Acta, Journal Year: 2024, Volume and Issue: 561, P. 119814 - 119814

Published: June 13, 2024

Language: Английский

---