IGF2BP3/ESM1/KLF10/BECN1 positive feedback loop: a novel therapeutic target in ovarian cancer via lipid metabolism reprogramming DOI Creative Commons
Anbo Gao, Juan Zou, Tian Zeng

et al.

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 17, 2025

Abstract Ovarian cancer (OC) is often detected at an advanced stage and has a high recurrence rate after surgery or chemotherapy. Thus, it essential to develop new strategies for OC treatment. This study tended investigate the effects of endothelial cell-specific molecule 1 (ESM1) in OC. The impact ESM1 on lipid metabolism was investigated through regulation expression. Differential genes regulated by were screened mRNA sequencing. role autophagy explored using inhibitor chloroquine (CQ). Co-IP, dual-luciferase reporter assay, actinomycin D treatment others used analyze mechanism metabolism. xenograft mouse model constructed explore development. regulatory patient samples verified microarray analysis Log-rank (Mantel-Cox) test. After silencing, cholesterol synthesis decreased lipolysis increased. sequencing revealed that related Beclin (BECN1). In vitro experiments, inhibited suppressing BECN1-mediated autophagy. BECN1 expression transcription factor Kruppel-like 10 (KLF10). competitive binding between HSPA5 promoted ubiquitination degradation HMGCR, thereby inhibiting production. intervention experiment with exogenous showed positive correlation m6A reader IGF2BP3 content. Mechanistically, stability mRNA. vivo modified methylation lipolysis. High predicted poor prognosis patients. IGF2BP3/ESM1/KLF10/BECN1 feedback, which promising target

Language: Английский

Free fatty acids derived from lipophagy enhanced resistance to anoikis by activating Src in high-invasive clear cell renal cell carcinoma cells DOI
Mengmeng Wu,

Guijuan Chen,

Xin Li

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111622 - 111622

Published: Jan. 1, 2025

Language: Английский

Citations

0
PLIN1 suppresses glioma progression through regulating lipid metabolism DOI Creative Commons

Kui Luo,

Kai Zhuang, Hao Wu

et al.

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 27, 2025

Abstract Glioma is a common and destructive brain tumor, which highly heterogeneous with poor prognosis. Developing diagnostic prognostic markers to identify treat glioma early would significantly improve the therapeutic outcomes. Here, we conducted RNA next-generation sequencing 33 samples 15 normal samples. We found Perilipin 1 (PLIN1) downregulated in correlated poorer outcome. Subsequent experiments revealed that up regulation of PLIN1 led repressed cell growth invasion glioma. Moreover, overexpression increased lipid accumulation cells, increasing expression biosynthesis related genes decreasing lipolysis genes. Mechanically, PI3K/AKT axis could regulate levels glioma, inhibition activity increase In conclusion, dysregulation downregulation following tumor proliferation, metabolism reprogramming

Language: Английский

Citations

0
Diagnosis, Prognosis, and Treatment of Triple-Negative Breast Cancer: A Review DOI Creative Commons

Huan Jie,

Wenhui Ma,

Cong Huang

et al.

Breast Cancer Targets and Therapy, Journal Year: 2025, Volume and Issue: Volume 17, P. 265 - 274

Published: March 1, 2025

Triple-negative breast cancer (TNBC) has become the most aggressive and worst prognostic subtype of due to lack estrogen receptor, progesterone receptor HER2 expression. This article systematically reviews progress in diagnosis, prognosis treatment TNBC. In terms imaging techniques (such as dynamic contrast-enhanced MRI multimodality ultrasound) combined with histological immunohistochemical detection Ki-67, PD-L1 expression) can improve early diagnosis rate; molecular markers (PIM-1, miR-522) classification (LAR, IM, BLIS, MES) provide basis for accurate classification. Prognostic evaluation requires a combination clinicopathologic features (tumor size, lymph node metastasis, tumor-to-stroma ratio), characteristics (BRCA mutation, expression), scoring systems. strategies, chemotherapy remains basis, but efficacy side effects need be balanced; neoadjuvant pathological complete response rate, while circulating tumor cells) help predict efficacy. targeted therapy, PARP inhibitors are significantly effective patients BRCA mutations, antibody drug conjugates (eg, sacituzumab govitecan) new options chemoresistant patients. immunotherapy, PD-1/PD-L1 improved progression-free survival, especially PD-L1-positive Combined metabolic reprogramming, individualized strategies further explored future overcome heterogeneity resistance emphasizes key role multidisciplinary collaboration precision medicine optimizing TNBC management provides an important reference clinical practice research direction.

Language: Английский

Citations

0
IGF2BP3/ESM1/KLF10/BECN1 positive feedback loop: a novel therapeutic target in ovarian cancer via lipid metabolism reprogramming DOI Creative Commons
Anbo Gao, Juan Zou, Tian Zeng

et al.

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 17, 2025

Abstract Ovarian cancer (OC) is often detected at an advanced stage and has a high recurrence rate after surgery or chemotherapy. Thus, it essential to develop new strategies for OC treatment. This study tended investigate the effects of endothelial cell-specific molecule 1 (ESM1) in OC. The impact ESM1 on lipid metabolism was investigated through regulation expression. Differential genes regulated by were screened mRNA sequencing. role autophagy explored using inhibitor chloroquine (CQ). Co-IP, dual-luciferase reporter assay, actinomycin D treatment others used analyze mechanism metabolism. xenograft mouse model constructed explore development. regulatory patient samples verified microarray analysis Log-rank (Mantel-Cox) test. After silencing, cholesterol synthesis decreased lipolysis increased. sequencing revealed that related Beclin (BECN1). In vitro experiments, inhibited suppressing BECN1-mediated autophagy. BECN1 expression transcription factor Kruppel-like 10 (KLF10). competitive binding between HSPA5 promoted ubiquitination degradation HMGCR, thereby inhibiting production. intervention experiment with exogenous showed positive correlation m6A reader IGF2BP3 content. Mechanistically, stability mRNA. vivo modified methylation lipolysis. High predicted poor prognosis patients. IGF2BP3/ESM1/KLF10/BECN1 feedback, which promising target

Language: Английский

Citations

0