Immunohistochemical Profiling of IDO1 and IL4I1 in Head and Neck Squamous Cell Carcinoma: Interplay for Metabolic Reprogramming? DOI Open Access
Benedikt Schmidl,

Maren Lauterbach,

Fabian Stögbauer

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3719 - 3719

Published: April 15, 2025

Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous malignant disease with limited number of biomarkers insufficient targeted therapies. The current therapeutic landscape challenged by low response rates, underscoring the need for new targets. success immunotherapy in HNSCC has highlighted importance immune microenvironment, since metabolic reprogramming, especially altered tryptophan metabolism, an important aspect evasion, interplay two enzymes IDO1 IL4I1 was investigated to assess their immunosuppressive roles potential as prognostic biomarkers. immunohistochemical expression evaluated experienced head pathologist tissue microarray (TMA) 402 patients HNSCC. Clinical pathological data were retrieved, overall survival calculated. In this study, expressed tumor cells TMA patients. analysis clinical revealed that high significantly associated worse OS (p = 0.0073), while did not reach statistical significance 0.087). combination both markers led clinically significant stratification Especially p16-negative OPSCC demonstrated poor survival. Immunologic differences between detected 403 patients, being A better have effect. Additional studies are necessary investigate complex reprogramming cells.

Language: Английский

Guanylate binding protein 5 is an immune‐related biomarker of oral squamous cell carcinoma: A retrospective prognostic study with bioinformatic analysis DOI Creative Commons

Masayo Hasegawa,

Yusuke Amano, Atsushi Kihara

et al.

Cancer Medicine, Journal Year: 2024, Volume and Issue: 13(13)

Published: July 1, 2024

Abstract Background Cancer utilizes immunosuppressive mechanisms to create a tumor microenvironment favorable for its progression. The purpose of this study is histologically characterize the immunological properties oral squamous cell carcinoma (OSCC) and identify key molecules involved in patient prognosis. Methods First, overlapping differentially expressed genes (DEGs) were screened from OSCC transcriptome data public databases. Correlation analysis DEGs with known immune‐related identified immune OSCC. Next, stromal patterns classified immunohistochemical staining was performed markers (CD3, CD4, Foxp3, CD8, CD20, CD68, CD163), programmed death‐ligand 1 (PD‐L1), guanylate binding protein 5 (GBP5) resected specimens obtained 110 patients who underwent resection. Correlations between each factor their prognostic impact analyzed. Results Among novel OSCC‐specific (including ADAMDEC1 , CXCL9 CXCL13 DPT GBP5 IDO1 PLA2G7 ), selected as target gene. Histopathologic showed that multiple T‐cell subsets CD20‐positive cells less common advanced stages, whereas CD163‐positive more stages. immature type pattern category associated infiltration, lower expression PD‐L1 cells, stroma, shorter overall survival recurrence‐free survival. Expression stroma correlated infiltration tumors cells. Patients low high had significantly longer Conclusions may reflect both invasive immunomodulatory potentials cancer‐associated fibroblasts has been suggested potential biomarker predict prognosis therapeutic efficacy checkpoint inhibitors.

Language: Английский

Citations

5
Clinicopathological and prognostic significance of stromal p16 and p53 expression in oral squamous cell carcinoma DOI Creative Commons
Yusuke Amano,

Masayo Hasegawa,

Atsushi Kihara

et al.

Annals of Diagnostic Pathology, Journal Year: 2025, Volume and Issue: 75, P. 152439 - 152439

Published: Jan. 15, 2025

Language: Английский

Citations

0
Regulation of Exosomal miR‐320d/FAM49B Axis by Guanylate Binding Protein 5 Promotes Cell Growth and Tumor Progression in Oral Squamous Cell Carcinoma DOI Creative Commons
Kai‐Fang Hu, Chih‐Wen Shu, Hung-Chih Chen

et al.

Journal of Oral Pathology and Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

ABSTRACT Background Guanylate binding protein 5 (GBP5) and exosomal miRNAs are involved in tumor progression. While several studies reveal the connection between GBP5 exosomes for immune response infection, this relationship cancer, particularly oral squamous cell carcinoma (OSCC), remains unexplored. Methods The miRNA extracted from cells was analyzed using next‐generation sequencing. Bioinformatic tools were used to predict target genes. OSCC growth verified by colony formation, viability, cycle analysis. Cancer Genome Atlas database inspect prognosis of patients. Results Our results showed that treated with GBP5‐silenced reduced formation. Also, 56 differentially expressed found compared scrambled cells. Among them, miR‐320d exhibited highest negative correlation High GBP5/low co‐expression linked disease‐free survival (DFS) patients OSCC. Interestingly, inhibitory effect on reversed inhibitors. Moreover, five genes predicted, only Family Sequence Similarity 49, Member B (FAM49B) a miR‐320d. A decreased level FAM49B derived cells, while Silencing enhanced cytotoxicity paclitaxel. abundantly tissues, high FAM49B/low GBP5/high DFS Conclusion study suggests downregulated may trigger expression facilitate

Language: Английский

Citations

0
Role of guanylate-binding protein 5 in inflammatory diseases, immune diseases, cancers, and its potential therapeutic implications DOI

Xufan Sun,

Guiyuan Jin, Huihui Zhou

et al.

Inflammopharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

Language: Английский

Citations

0
Immunohistochemical Profiling of IDO1 and IL4I1 in Head and Neck Squamous Cell Carcinoma: Interplay for Metabolic Reprogramming? DOI Open Access
Benedikt Schmidl,

Maren Lauterbach,

Fabian Stögbauer

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3719 - 3719

Published: April 15, 2025

Head and neck squamous cell carcinoma (HNSCC) is a heterogeneous malignant disease with limited number of biomarkers insufficient targeted therapies. The current therapeutic landscape challenged by low response rates, underscoring the need for new targets. success immunotherapy in HNSCC has highlighted importance immune microenvironment, since metabolic reprogramming, especially altered tryptophan metabolism, an important aspect evasion, interplay two enzymes IDO1 IL4I1 was investigated to assess their immunosuppressive roles potential as prognostic biomarkers. immunohistochemical expression evaluated experienced head pathologist tissue microarray (TMA) 402 patients HNSCC. Clinical pathological data were retrieved, overall survival calculated. In this study, expressed tumor cells TMA patients. analysis clinical revealed that high significantly associated worse OS (p = 0.0073), while did not reach statistical significance 0.087). combination both markers led clinically significant stratification Especially p16-negative OPSCC demonstrated poor survival. Immunologic differences between detected 403 patients, being A better have effect. Additional studies are necessary investigate complex reprogramming cells.

Language: Английский

Citations

0