The Anti-Inflammatory Effects and Molecular Mechanism of Citri Reticulatae Pericarpium Essential Oil: A Combined GC-MS and Network Pharmacology Study DOI Creative Commons

Junmei Pu,

Jiabao Cui,

Hui Yang

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(9), P. 1455 - 1455

Published: April 23, 2025

This study investigated the chemical composition and anti-inflammatory effects of essential oils extracted from Citrus aurantium flower, sinensis, Brazilian limon, bergamia, Citri Reticulatae Pericarpium using steam distillation gas chromatography-mass spectrometry (GC-MS). Their activities were assessed in LPS-stimulated RAW 264.7 cells. Among them, oil (CRPEO) exhibited most potent effects, with D-Limonene (76.51%), α-Pinene (2.68%), Linalool (2.11%) as its primary constituents. The CCK-8 assay showed that no cytotoxicity on HaCaT cells at a concentration 50 μg/mL. CRPEO significantly preserved cell viability reduced production pro-inflammatory mediators, including tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, nitric oxide (NO). Gene expression analysis via RT-qPCR further confirmed downregulation TNF-α, IL-6, inducible synthase (iNOS) mRNA level. Network pharmacology molecular docking studies employed to identify α-Bulnesene key bioactive component revealed principal target is NLR Family Pyrin Domain-Containing 3 (NLRP3) inflammasome. These findings highlight strong potential suggest promising therapeutic application for inflammation-related conditions.

Language: Английский

The Anti-Inflammatory Effects and Molecular Mechanism of Citri Reticulatae Pericarpium Essential Oil: A Combined GC-MS and Network Pharmacology Study DOI Creative Commons

Junmei Pu,

Jiabao Cui,

Hui Yang

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(9), P. 1455 - 1455

Published: April 23, 2025

This study investigated the chemical composition and anti-inflammatory effects of essential oils extracted from Citrus aurantium flower, sinensis, Brazilian limon, bergamia, Citri Reticulatae Pericarpium using steam distillation gas chromatography-mass spectrometry (GC-MS). Their activities were assessed in LPS-stimulated RAW 264.7 cells. Among them, oil (CRPEO) exhibited most potent effects, with D-Limonene (76.51%), α-Pinene (2.68%), Linalool (2.11%) as its primary constituents. The CCK-8 assay showed that no cytotoxicity on HaCaT cells at a concentration 50 μg/mL. CRPEO significantly preserved cell viability reduced production pro-inflammatory mediators, including tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-1β, nitric oxide (NO). Gene expression analysis via RT-qPCR further confirmed downregulation TNF-α, IL-6, inducible synthase (iNOS) mRNA level. Network pharmacology molecular docking studies employed to identify α-Bulnesene key bioactive component revealed principal target is NLR Family Pyrin Domain-Containing 3 (NLRP3) inflammasome. These findings highlight strong potential suggest promising therapeutic application for inflammation-related conditions.

Language: Английский

Citations

0