Carvacrol is a Novel Natural Therapeutic Approach Through the Inhibition of Proliferation, Autophagy and Migration in Pancreatic Ductal Adenocarcinoma Cells DOI Open Access
Zeynep Akpınar, Nilgün Gürbüz

SDÜ Tıp Fakültesi Dergisi, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Objective: Pancreatic ductal adenocarcinoma, which is the most common and aggressive pancreatic cancer, has highest mortality rate of cancers because difficulties in diagnosis chemoresistance. As chemotherapeutic options are very limited insufficient for PDAC, novel effective therapeutic approaches urgently needed cancer patients. Carvacrol naturally found thyme (Thymus vulgaris), wild bergamot (Citrus aurantium var. bergamia Loisel), black cumin (Nigella sativa), marjoram (Origanum scabrum, Origanum microphyllum, onites, vulgare) pepper (Lepidium flavum) plants shown to have antibacterial antioxidant effects. In this study, we aimed investigate anticarcinogenic potential carvacrol through proliferation autophagy PDAC cells. Material Method: To determine anti-proliferative effects Panc-1 cells, performed MTS assay using different doses 100, 200, 300, 400, 500, 600, 700 800 μM at 24h, 48h, 72h. The gene protein expressions Atg16L1 Beclin-1, key mediators, were analyzed by RT-PCR western blot cells treated with 300 400 48h. Additionally, migrative property was evaluated a wound healing assay. Results: Based on results, significantly inhibited cell 24h 48 h Panc-1. These same led decreased migration Atg16L1, Beclin-1 expressions. Conclusion: Our findings first revealed that promising value as approach PDAC. We believe further mechanistic investigations will be guide its clinical usage.

Language: Английский

Biogenic synthesis copper oxide nanoparticle from Artemisia judaica and their evaluation of biological and photocatalytic activities DOI

Rahaf Mohammed Salem Al-Masoudi,

Chellasamy Panneerselvam,

Hajer Feraij A Alhawiti

et al.

Journal of Industrial and Engineering Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Research progress on the signaling pathway mechanism of terpenoids against breast cancer DOI Creative Commons

S. Tu,

Fen Hu, Juan Zhang

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 31, 2025

Language: Английский

Citations

0
Evaluation of Carvacrol Zinc Oxide Quantum Dots in Mitigating Hepatic Inflammation and Function Impairment in DMBA‐Induced Mammary Carcinogenesis DOI
Manoj Kumar Srinivasan, Briska Jifrina Premnath, N. Nalini

et al.

Journal of Biochemical and Molecular Toxicology, Journal Year: 2025, Volume and Issue: 39(5)

Published: May 1, 2025

ABSTRACT The liver plays a crucial role in metabolizing and purging various substances from the body. Exposure to toxins like DMBA (7,12‐dimethylbenz[a]anthracene) can harm liver, leading inflammation, impaired function, potential development of lesions or tumors. present study explored protective effect CVC‐ZnO QDs (carvacrol‐zinc oxide quantum dots) on by DMBA‐induced mammary carcinoma. Female Sprague Dawley rats were used, cancer was initiated injecting near gland. Different concentrations administered orally determine most effective dosage. Various tissue factors evaluated, including marker enzymes, antioxidant status, lipid peroxidation, detoxification enzyme activities protein bound carbohydrates. Additionally, inflammatory response investigated using immunohistochemistry PCR. Results revealed that treated with showed significant decrease peroxidation levels, Phase I also increased II activity, levels compared solely DMBA. Histopathological analysis confirmed shielded damage. Furthermore, found reduce expression IL‐6, NF‐κB, COX‐2 rats. Overall, demonstrated administering at dose 4 mg/kg b.w had notable hepatoprotective against

Language: Английский

Citations

0
Evaluating the in vitro and in vivo effects of carvacrol zinc oxide quantum dots in breast cancer DOI
Manoj Kumar Srinivasan, N. Nalini

Journal of Biomaterials Science Polymer Edition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 20

Published: Dec. 3, 2024

The study investigates the molecular interactions and biological effects of carvacrol zinc oxide quantum dots (CVC-ZnO QDs) on breast cancer in vitro MCF-7 cell lines vivo mammary models. Molecular docking using AutoDock Vina revealed binding energies CVC with key proteins PI3K/AKT/mTOR pathway, including PI3K, AKT, PTEN, mTOR. results showed significant interaction specific amino acids, indicating a strong affinity. In studies demonstrated dose-dependent cytotoxic effect CVC-ZnO QDs cells, an IC50 20.02 µg/mL, while enhancing intracellular reactive oxygen species (ROS) decreasing mitochondrial membrane potential (MMP), indicative apoptosis induction. Antioxidant activity, lipid peroxidation, nuclear morphological changes were assessed, revealing decreased antioxidant status increased peroxidation treated cells. vivo, modulated signaling DMBA-induced rats, p-PI3K, p-AKT, p-mTOR expression upregulating PTEN. Immunohistochemistry, qRT-PCR, Western blot analyses confirmed these alterations. concludes that exert pro-apoptotic cells by modulating PI3K/Akt/mTOR pathway promoting oxidative stress, presenting therapeutic strategy for management.

Language: Английский

Citations

1
Breast Cancer Treatment: The Potential of Organic and Inorganic Nanocarriers in Targeted Drug Delivery DOI Creative Commons

Saravanan Alamelu,

Kamalesh Balakumar Venkatesan, Kalist Shagirtha

et al.

Drugs and Drug Candidates, Journal Year: 2024, Volume and Issue: 3(4), P. 813 - 837

Published: Nov. 25, 2024

Breast cancer (BC) is the most prevalent form of malignancy among women on a global scale, ranking alongside lung cancer. Presently, conventional approaches to treatment include surgical procedures followed by chemotherapy or radiotherapy. Nonetheless, efficacy these treatments in battling BC often compromised due adverse effects they inflict healthy tissues and organs. In recent times, range nanoparticles (NPs) has emerged, exhibiting potential specifically target malignant cells while sparing normal organs from harm. This paved way for development nanoparticle-mediated targeted drug delivery systems, holding great promise as technique addressing BC. To increase this new method, several nanocarriers including inorganic NPs (such magnetic NPs, silica etc.) organic (e.g., dendrimers, liposomes, micelles, polymeric NPs) have been used. Herein, we discuss mechanism NP-targeted advancement therapeutic strategies anticancer We also future prospects challenges nanoparticle-based therapies

Language: Английский

Citations

0
Pluronic F-127 coated zinc oxide nanoparticles from Gynura pseudochina : a comprehensive assessment of antibacterial, and anticancer activities DOI

Murugan A. Munusamy,

Muruganantham Bharathi, Abdullah A. Alarfaj

et al.

Journal of Biomaterials Science Polymer Edition, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 20

Published: Dec. 14, 2024

The study synthesized Pluronic F-127 coated zinc oxide nanoparticles (PF-127ZnO NPs) using

Language: Английский

Citations

0
Carvacrol is a Novel Natural Therapeutic Approach Through the Inhibition of Proliferation, Autophagy and Migration in Pancreatic Ductal Adenocarcinoma Cells DOI Open Access
Zeynep Akpınar, Nilgün Gürbüz

SDÜ Tıp Fakültesi Dergisi, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Objective: Pancreatic ductal adenocarcinoma, which is the most common and aggressive pancreatic cancer, has highest mortality rate of cancers because difficulties in diagnosis chemoresistance. As chemotherapeutic options are very limited insufficient for PDAC, novel effective therapeutic approaches urgently needed cancer patients. Carvacrol naturally found thyme (Thymus vulgaris), wild bergamot (Citrus aurantium var. bergamia Loisel), black cumin (Nigella sativa), marjoram (Origanum scabrum, Origanum microphyllum, onites, vulgare) pepper (Lepidium flavum) plants shown to have antibacterial antioxidant effects. In this study, we aimed investigate anticarcinogenic potential carvacrol through proliferation autophagy PDAC cells. Material Method: To determine anti-proliferative effects Panc-1 cells, performed MTS assay using different doses 100, 200, 300, 400, 500, 600, 700 800 μM at 24h, 48h, 72h. The gene protein expressions Atg16L1 Beclin-1, key mediators, were analyzed by RT-PCR western blot cells treated with 300 400 48h. Additionally, migrative property was evaluated a wound healing assay. Results: Based on results, significantly inhibited cell 24h 48 h Panc-1. These same led decreased migration Atg16L1, Beclin-1 expressions. Conclusion: Our findings first revealed that promising value as approach PDAC. We believe further mechanistic investigations will be guide its clinical usage.

Language: Английский

Citations

0