Creating overlapping genes by alternate-frame insertion DOI Open Access
Sean P. Leonard, Tiffany M. Halvorsen, Bentley Lim

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Abstract Overlapping genes–wherein two different proteins are translated from alternative frames of the same DNA sequence–provide a means to stabilize an engineered gene by directly linking its evolutionary fate with that overlapped gene. However, creating overlapping pairs is challenging as it requires redesign both protein products accommodate overlap constraints. Here, we present new “ o verlapping, lternate- f rame insertion” (OAFI) method for genes insertion “inner” gene, encoded in alternate frame, into flexible region “outer” Using OAFI, create bacterial toxins within antibiotic resistance We show inner and outer retain functionality despite redesign, translation influenced position Additionally, selection alters permitted inactivating mutations can restrict horizontal transfer Overall, OAFI offers versatile tool synthetic biology, expanding applications stabilization biocontainment.

Language: Английский

Creating overlapping genes by alternate-frame insertion DOI Open Access
Sean P. Leonard, Tiffany M. Halvorsen, Bentley Lim

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 7, 2024

Abstract Overlapping genes–wherein two different proteins are translated from alternative frames of the same DNA sequence–provide a means to stabilize an engineered gene by directly linking its evolutionary fate with that overlapped gene. However, creating overlapping pairs is challenging as it requires redesign both protein products accommodate overlap constraints. Here, we present new “ o verlapping, lternate- f rame insertion” (OAFI) method for genes insertion “inner” gene, encoded in alternate frame, into flexible region “outer” Using OAFI, create bacterial toxins within antibiotic resistance We show inner and outer retain functionality despite redesign, translation influenced position Additionally, selection alters permitted inactivating mutations can restrict horizontal transfer Overall, OAFI offers versatile tool synthetic biology, expanding applications stabilization biocontainment.

Language: Английский

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