Direct and Ultrasensitive Bioluminescent Detection of Intact Respiratory Viruses DOI Creative Commons
Alexander Gräwe, Harmen J. van der Veer, Seino A. K. Jongkees

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(10), P. 5550 - 5560

Published: Oct. 8, 2024

Respiratory viruses such as SARS-CoV-2, influenza, and respiratory syncytial virus (RSV) represent pressing health risks. Rapid diagnostic tests for these detect single antigens or nucleic acids, which do not necessarily correlate with the amount of intact virus. Instead, specific detection particles may be more effective at assessing contagiousness a patient. Here, we report GLOVID, modular biosensor platform to virions against background "free" viral proteins in solution. Our approach harnesses multivalent display distinct on surface particle template reconstitution split luciferase, allowing specific, single-step influenza A RSV corresponding 0.1–0.3 fM genomic units. The protein ligation system used assemble GLOVID sensors is compatible broad range binding domains, including nanobodies, scFv fragments, cyclic peptides, allows straightforward adjustment sensor target different viruses.

Language: Английский

Quantitative approaches for studying G protein-coupled receptor signalling and pharmacology DOI Creative Commons
Abigail Pearce,

Theo Redfern-Nichols,

E. J. Wills

et al.

Journal of Cell Science, Journal Year: 2025, Volume and Issue: 138(1)

Published: Jan. 1, 2025

ABSTRACT G protein-coupled receptor (GPCR) signalling pathways underlie numerous physiological processes, are implicated in many diseases and major targets for therapeutics. There more than 800 GPCRs, which together transduce a vast array of extracellular stimuli into variety intracellular signals via heterotrimeric protein activation multiple downstream effectors. A key challenge cell biology research the pharmaceutical industry is developing tools that enable quantitative investigation GPCR to gain mechanistic insights varied cellular functions pharmacology GPCRs. Recent progress this area has been rapid extensive. In Review, we provide critical overview these new, state-of-the-art approaches investigate pathways. These include novel sensors, Förster or bioluminescence resonance energy transfer assays, libraries tagged proteins transcriptional reporters. improved studies different stages signalling, including activation, second messenger (cAMP Ca2+) β-arrestin recruitment internalisation trafficking

Language: Английский

Citations

2
The Outputs of Molecular Sensors Detectable by Human Senses DOI Creative Commons
Maria S. Rubel, Tatiana Zemerova, Dmitry M. Kolpashchikov

et al.

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Molecular sensors respond to the presence of biological analytes by producing signals that are either directly perceivable human sensory systems or converted into electric signals, which require electronic devices for communicating humans. Here, we review outputs molecular detectable senses. According literature, with visual dominate. Undeservedly unnoticed, release gases might be particularly useful since gas output can detected several senses in a quantifiable format. Relatively new tactile accessed visually impaired people. their bypassing may contribute development point-of-care testing technologies, as well providing direct communication nanorobots

Language: Английский

Citations

0
Protein Silencing with Self-Peptides DOI

Anoop Philip,

Mayank Gupta, Shankha Banerjee

et al.

The Journal of Physical Chemistry B, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 13, 2025

Designing functional molecules which can recognize and modify the activity of a specific protein is frequently encountered challenge in biology pharmaceutical chemistry, requires major effort for each target. Here we demonstrate that "self-peptides", parts folded proteins by their nature are recognizable rest protein, provide general route to developing such molecules. Such synthetic peptide with chemically prestabilized conformation incorporate into target during its folding, potentially displace native counterpart cause deficits. This strategy especially promising β-barrel topology, as seam barrel provides vulnerable We this using green fluorescent (EGFP) model, fluorescence direct reporter function. Refolding EGFP presence 35 μM disulfide-stabilized 20-residue self-peptide (SP1, resembles seam, strands 3 11, GFP) quenches 97%. A same composition but different sequence only 40% effective, demonstrating silencing relatively specific. Fluorescence correlation spectroscopy time-resolved lifetime measurements show SP1 causes complete long-term it incorporates into. result principle have biological application if synthesis, before nascent folds. indeed silence sfGFP (closely related EGFP) ribosomal synthesis an vitro translation system. Therefore, self-peptides present protein-specific silencers physiological applications.

Language: Английский

Citations

0
Advancing Endosomal Escape of Polymeric Nanoparticles: Towards Improved Intracellular Delivery DOI
Yufu Wang,

Vajini Ukwattage,

Yijun Xiong

et al.

Materials Horizons, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Polymeric nanoparticles have emerged as a promising platform for the intracellular delivery of therapeutics, offering unique advantages such tunable chemical properties and stimuli-responsive behavior. However, significant challenge in their use remains efficient therapeutic cargo to site action. This typically relies on escape from endosomal/lysosomal compartments where are trapped upon internalisation within cell, process termed endosomal escape. Despite considerable research, mechanisms underlying still poorly understood, with inconsistent findings across studies. Moreover, there is notable lack standardized methods accurately quantify this process. In review, we explore current understanding specific polymeric critical design strategies that been used. We also highlight recent advancements escape, aim promoting broader application these technologies behaviour nanoparticles.

Language: Английский

Citations

0
Biosensing strategies using recombinant luminescent proteins and their use for food and environmental analysis DOI
Fernando Pradanas-González, Marta García-Cortés, Bettina Glahn-Martínez

et al.

Analytical and Bioanalytical Chemistry, Journal Year: 2024, Volume and Issue: 416(30), P. 7205 - 7224

Published: Sept. 26, 2024

Language: Английский

Citations

1
Direct and Ultrasensitive Bioluminescent Detection of Intact Respiratory Viruses DOI Creative Commons
Alexander Gräwe, Harmen J. van der Veer, Seino A. K. Jongkees

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(10), P. 5550 - 5560

Published: Oct. 8, 2024

Respiratory viruses such as SARS-CoV-2, influenza, and respiratory syncytial virus (RSV) represent pressing health risks. Rapid diagnostic tests for these detect single antigens or nucleic acids, which do not necessarily correlate with the amount of intact virus. Instead, specific detection particles may be more effective at assessing contagiousness a patient. Here, we report GLOVID, modular biosensor platform to virions against background "free" viral proteins in solution. Our approach harnesses multivalent display distinct on surface particle template reconstitution split luciferase, allowing specific, single-step influenza A RSV corresponding 0.1–0.3 fM genomic units. The protein ligation system used assemble GLOVID sensors is compatible broad range binding domains, including nanobodies, scFv fragments, cyclic peptides, allows straightforward adjustment sensor target different viruses.

Language: Английский

Citations

0