Single-molecule protein sequencing with nanopores
Nature Reviews Bioengineering,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 21, 2024
Language: Английский
Fabricating Reproducible, Reversible, and High Signal Change Aptasensors with Gold-Modified Nanopipettes
ACS Applied Materials & Interfaces,
Journal Year:
2025,
Volume and Issue:
unknown
Published: April 21, 2025
Aptamer-functionalized
nanopipettes
are
an
emerging
class
of
biosensors
for
the
label-free
detection
specific
molecules.
While
various
strategies
exist
immobilizing
single-stranded
DNA
aptamers
onto
inner
walls
glass
nanopipettes,
impact
fabrication
method
on
sensor
sensitivity,
signal
change,
reproducibility,
and
reliability
remains
unexplored.
In
this
study,
we
compared
three
methods
found
that
sensors
fabricated
using
gold
nanoparticles
(AuNPs)
synthesized
within
produced
most
reproducible
results
while
also
allowing
control
over
modification
process.
contrast,
two
other
aptamer
immobilization
methods,
which
relied
multistep
polymer
coatings
with
aminated
or
thiolated
coupling,
were
hindered
by
water
sensitivity
uneven
deposition,
resulting
in
inconsistent
responses.
Using
AuNP-coated
successfully
numerous
varying
sizes,
demonstrating
smaller
produce
greater
changes.
Sensors
constructed
diameters
ranging
from
22
to
30
nm
exhibited
large
changes
(>40%)
when
AuNP
synthesis
particles
near
tip
opening
without
causing
blockage.
However,
observed
significantly
lower
(using
same-sized
nanopipettes),
attributed
either
minimal
Au
present
at
conversely
blocked
probe.
These
highlight
critical
role
maximizing
enhancing
identifying
how
why
fail.
This
work
aims
facilitate
broader
adoption
aptamer-functionalized
analytical
sensing
applications.
Language: Английский
Specific ATP Detection Using Molecule‐Responsive DNA Nanopores
Hiromu Akai,
No information about this author
Hirano Taichi,
No information about this author
Takuya Mabuchi
No information about this author
et al.
Small,
Journal Year:
2025,
Volume and Issue:
unknown
Published: May 2, 2025
Abstract
Nanopore
sensing
is
a
powerful
single‐molecule
measurement
technique
with
label‐free
characteristics
and
high
spatiotemporal
resolution.
Although
nanopores
tailored
to
target
molecules
in
terms
of
shape
molecular
selectivity
are
required
for
highly
sensitive
measurements,
biological
solid‐state
used
conventional
nanopore
exhibit
limited
functionalization.
Therefore,
synthetic
DNA
constructed
using
structural
nanotechnology
attracting
attention
as
alternatives
because
their
designability
the
possibility
However,
stochastic
functionalized
has
not
been
achieved.
In
this
study,
we
developed
molecule‐responsive
an
adenosine
triphosphate
(ATP)‐binding
split
aptamer
that
can
repeatedly
open
close
pore
entrance
by
binding
dissociation
between
ATP.
ATP
measurements
demonstrated
ATP‐responsive
nanopore.
This
study
believes
sensors
based
on
be
analytical
tool
drug
discovery
disease
diagnosis.
Language: Английский
Metal–Organic Framework Sub-Nanochannels within the Confined Micropipettes: Precise Construction Makes It a Universal Aptamer-Based Sensing Platform
Haoran Tang,
No information about this author
Shuai Zhang,
No information about this author
Binbin Yang
No information about this author
et al.
Analytical Chemistry,
Journal Year:
2024,
Volume and Issue:
96(44), P. 17649 - 17656
Published: Oct. 22, 2024
It
is
crucial
to
precisely
construct
metal-organic
framework
(MOF)
sub-nanochannels
at
the
tip
of
micro/nanopipettes
for
fundamental
research
and
sensing
applications.
The
quality
MOF
modification
plays
a
significant
role
in
influencing
subsequent
research,
particularly
In
this
work,
we
present
precise
method
constructing
glass
micropipettes,
which
serve
as
universal
aptamer-based
platform
selective
detection
proteins.
Language: Английский