Robust Encoding of Precise Addressing Information on AuNPs to Direct Their Heritable Positioning on DNA Origami† DOI
Yuxi Li,

Jiang Chu,

Yanyu Zhou

et al.

Chinese Journal of Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 14, 2024

Comprehensive Summary The programmability of DNA assembly allows for the creation gold nanostructures with custom shapes and tailored optical properties, holding promise applications including single‐molecule sensing imaging. While origami provides addressable site information structure construction, there have been few studies on encoding onto nanoparticles (AuNPs) to guide Au‐DNA structures, especially extensive large AuNPs ( e.g. , 30 nm). In this work, we introduce a strategy that encodes versatile ranging in size from 5 nm using template (parent origami). This encoded serves direct positioning within regenerated (daughter Our findings demonstrate successful same daughter as parent origami, yields up 80%. approach offers new possibilities achieving precise control over functionality.

Language: Английский

Dual Protein Corona-Mediated Target Recognition System for Visual Detection and Single-Molecule Counting of Nucleic Acids DOI

B M Zhang,

Pengbo Zhang, Hao Wang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Rapid, highly sensitive, and specific nucleic acid detection plays a crucial role in advancing point-of-care (POC) diagnostics for pathogens viruses, cancer monitoring, optimizing clinical treatments. Herein, leveraging the precise recognition ability of CRISPR/dCas9 powerful localized surface plasmon resonance (LSPR) gold nanoparticles (AuNPs), we report design dual protein corona-mediated platform to simultaneously fulfill rapid POC testing single-molecule counting acids one-pot one-step manner. This system uses guide RNA as molecular bridge anchor dCas9 onto AuNPs, forming artificial coronas. Upon recognizing target, interaction between two coronas on same molecule triggers cross-linked aggregation AuNPs. Then, target low 100 aM can be visually detected within 30 min, making particularly well-suited application screening emerging epidemics. Additionally, superior LSPR properties AuNPs increase light-scattering signal generated during target-induced aggregation, enabling visualization aggregated diffraction-limited spots under confocal microscopy. By these spots, achieves unprecedented sensitivity, identifying 1 aM, which is equivalent just 6 molecules 10 μL system, demonstrating capability. offers exceptional promise large-scale pathogenic viruses early cancer, applications requiring ultrahigh sensitivity at level.

Language: Английский

Citations

2
Harnessing nanotechnology for cancer treatment DOI Creative Commons
Jiajun Zhu,

H. H. Lee,

Rong Huang

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 20, 2025

Nanotechnology has become a groundbreaking innovation force in cancer therapy, offering innovative solutions to the limitations of conventional treatments such as chemotherapy and radiation. By manipulating materials at nanoscale, researchers have developed nanocarriers capable targeted drug delivery, improving therapeutic efficacy while reducing systemic toxicity. Nanoparticles like liposomes, dendrimers, polymeric nanomaterials shown significant promise delivering chemotherapeutic agents directly tumor sites, enhancing bioavailability minimizing damage healthy tissues. In addition with utilization tools quantum dots nanosensors that enables more precise identification biomarkers, nanotechnology is also playing pivotal role early detection diagnosis. Furthermore, nanotechnology-based strategies, including photothermal gene therapy immunotherapy are novel ways combat by selectively targeting cells immune response. Nevertheless, despite these progressions, obstacles still persist, particularly clinical translation technologies. Issues nanoparticle toxicity, biocompatibility, complexity regulatory approval hinder widespread adoption nanomedicine oncology. This review discusses different applications highlighting its potential hurdles implementation. Future research needs concentrate on addressing unlock full providing personalized, effective, minimally invasive treatments.

Language: Английский

Citations

1
A lateral flow assay based on aptamer for the detection of AFM1 in milk samples DOI
Yibo Wu,

Mingwei Qin,

Peifang Chen

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106175 - 106175

Published: Feb. 1, 2025

Language: Английский

Citations

1
Loading monocytes with magnetic nanoparticles enables their magnetic control without toxicity DOI Creative Commons

Laura Mödl,

Lucas R. Carnell,

René Stein

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2025, Volume and Issue: 12

Published: Jan. 8, 2025

With the help of superparamagnetic iron oxide nanoparticles (SPIONs), cells can be magnetically directed so that they accumulated at target sites. This principle used to make monocytes steerable in order improve tumor accumulation, e.g., for immunotherapy with chimeric antigen receptor (CAR) monocytes. Here, we investigated loading monocytic THP-1 SPIONs, analyzed their impact on viability and cellular reactive oxygen species (ROS) generation magnetic enrichment. Finally, compared confirmed experimentally generated enrichment data computational simulations. When were incubated citrate-coated SPIONs (SPIONCitrate) or citrate-stabilized gold-coated (SPIONGold), ingested particles, as determined via transmission electron microscopy atomic emission spectroscopy. Flow cytometry showed particles biocompatible produced hardly any ROS. SPION-loading, Ibidi flow slides edge a Neodym magnet, where field force maximal, calculated by our model. successfully loaded which exhibited excellent biocompatibility provided steerability. The model predicted actual accumulation SPION-loaded cells, enabling more systematic faster exploration design space future.

Language: Английский

Citations

0
Electrostatic action promotes bonding efficacy of DNA-modified nanoparticle self-assembly in solution DOI Creative Commons
Qiuyan Yu,

Jinhong Huang,

Mengyuan Liu

et al.

Results in Physics, Journal Year: 2025, Volume and Issue: unknown, P. 108153 - 108153

Published: Feb. 1, 2025

Language: Английский

Citations

0
Enhancing the Peroxidase-Mimicking Activity of Gold Nanoparticles for Lateral Flow Assays: Quantitative Evaluation in a Kinetic View DOI
Vasily G. Panferov,

Wenjun Zhang,

Nicholas D’Abruzzo

et al.

Langmuir, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 14, 2025

Highly sensitive lateral flow immunoassays (LFIAs) are essential for various point-of-care applications, and gold nanoparticles (Au NPs) by far the most commonly used labels. However, conventional LFIAs often suffer from high detection limits (LOD) or low sensitivity. In this study, we investigated three strategies to enhance sensitivity of improving peroxidase-mimicking (POD) activity Au NPs. The POD unmodified NPs was negligible (<0.01 units/mg, U/mg). first strategy involved coupling with horseradish peroxidase (HRP), which increased 65 U/mg. second approach forming a thin palladium iridium shell on NPs, elevated 0.69-0.71 third binding mercury ions (Hg2+) resulting in up 3 Finally, developed simple quantitative model estimate LOD based kinetic parameters. Using Au-HRP conjugates, demonstrated that experimentally measured consistent calculated values. provides framework evaluating catalytic signal amplification can be guide development highly assays.

Language: Английский

Citations

0
Recent Advances in Enzyme‐Mediated Rolling Circle Replication Technology Empowering Virus Detection DOI
‬Min Du, Juncheng Liu,

Guangshuai Teng

et al.

Chinese Journal of Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 11, 2025

Comprehensive Summary The prevalence and spread of viral infectious diseases pose a grave threat to global public health, particularly resulting in significant number casualties. To curb the diseases, virus testing is one efficient economical means, among which nucleic acid detection has advantage detecting infections at an early stage, even before symptoms appear. Rolling circle replication representative enzyme‐mediated isothermal amplification technology, characterized by its mild reaction conditions, no need for temperature control equipment, high efficiency. This review provides conceptual overview latest advances rolling (RCR) their applications detection, focusing on molecular design principles different application scenarios. first part briefly describes significance advantages RCR detection. second elaborates principle preparation strategy derivative technologies. third focuses various scenarios In end, we provide perspective innovation improving accuracy specificity cope with challenges that may arise future.

Language: Английский

Citations

0
Advances in Apolipoprotein-A4 Biosensing Assays for Depression Diagnosis DOI

Zahra Jamalizadeh Bahaabadi,

Sercan Karav, Amirhossein Sahebkar

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12

Published: April 29, 2025

Apolipoprotein-A4 (Apo-A4) is a plasma protein that plays role in various physiological and behavioral-emotional reactions when faced with stress. Studies have shown close relationship between Apo-A4 the onset of depression its symptoms. However, there currently no reliable laboratory approach to confirm diagnosis depression. Therefore, development precise effective technique assess might help early detection screening other related psychiatric diseases, as well tracking managing course treatment. As technology advances, biosensors become quick, accurate, sensitive tools for personal care illness diagnosis. Biosensors measuring detecting levels recently been designed. These studies emphasized accurate diagnostic measurement techniques. This review attempts give general overview introduce established measurement.

Language: Английский

Citations

0
Robust Encoding of Precise Addressing Information on AuNPs to Direct Their Heritable Positioning on DNA Origami† DOI
Yuxi Li,

Jiang Chu,

Yanyu Zhou

et al.

Chinese Journal of Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 14, 2024

Comprehensive Summary The programmability of DNA assembly allows for the creation gold nanostructures with custom shapes and tailored optical properties, holding promise applications including single‐molecule sensing imaging. While origami provides addressable site information structure construction, there have been few studies on encoding onto nanoparticles (AuNPs) to guide Au‐DNA structures, especially extensive large AuNPs ( e.g. , 30 nm). In this work, we introduce a strategy that encodes versatile ranging in size from 5 nm using template (parent origami). This encoded serves direct positioning within regenerated (daughter Our findings demonstrate successful same daughter as parent origami, yields up 80%. approach offers new possibilities achieving precise control over functionality.

Language: Английский

Citations

0