Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: May 9, 2025

The stimulus-response behavior of protocells under environmental osmotic pressure changes has long been a subject scientific inquiry. Herein, we demonstrate way to membranized coacervate microdroplets based on cholesterol anchoring phospholipids, which provides enhanced stability, enabling morphological transformations instead dissociation during subsequent changes. In hypotonic environments, these coacervates equilibrate through transient internal vacuole formation, concomitant with transmembrane substrate influx that triggers enzymatic reaction acceleration. By contrast, in hypertonic environment, the responds bursting-like deformation can then quickly recover due effect phospholipids. Notably, it is found such could even successfully induce endocytosis Staphylococcus aureus by coacervates. Furthermore, integration Coa@DMPC's responsiveness, actin polymerization activated endocytic S. achieved. Not only our proposed method phospholipid membranization contribute new model mimic more complex bionic structures, but also revealed response various expected help explain stress behaviors and emerging unique properties cells similar environments.

Language: Английский