Clinical Oral Investigations, Journal Year: 2023, Volume and Issue: 27(11), P. 6597 - 6606
Published: Sept. 22, 2023
Language: Английский
Cancers, Journal Year: 2024, Volume and Issue: 16(12), P. 2280 - 2280
Published: June 20, 2024
CtDNA is emerging as a non-invasive clinical detection method for several cancers, including genitourinary (GU) cancers such prostate cancer, bladder and renal cell carcinoma (RCC). assays have shown promise in early of GU providing prognostic information, assessing real-time treatment response, detecting residual disease relapse. The ease obtaining “liquid biopsy” from blood or urine enhances its potential to be used biomarker. Interrogating these biopsies” ctDNA can then detect common cancer mutations, novel genomic alterations, epigenetic modifications. has undergone investigation numerous trials, which could address needs instance, earlier RCC, therapeutic response prediction castration-resistant monitoring recurrence cancers. utilization liquid biopsy analysis provides promising advancing precision medicine within the field
Language: Английский
Citations
4
Therapeutic Advances in Medical Oncology, Journal Year: 2025, Volume and Issue: 17
Published: Jan. 1, 2025
Oropharyngeal carcinoma, a type of head and neck cancer (HNC), is an emerging malignancy associated with low survival rates. It typically affects older males correlates smoking, drinking, lower socioeconomic conditions. Traditional treatments such as surgery have often yielded limited outcomes. However, recent insights, particularly those emphasized by Dr Teknos in his Keynote Conference at MedNews Week, sparked deeper exploration into alternative more promising treatment methods. A comprehensive literature review was conducted to explore this subject further. One approach, demonstrated the UMCC 9921 trial, involves protocol starting induction chemotherapy. This initial phase aims reduce tumor burden assess response treatment. Based on individual outcomes, patients then undergo concurrent chemoradiation or salvage surgery. strategy has significantly improved rates, especially human papillomavirus (HPV)-positive patients, showcasing potential tailored treatments. While these advancements are promising, long-term complications dysphagia osteoradionecrosis remain cause concern. The rise HPV-related squamous cell carcinoma further changed how risk factors outcomes viewed. HPV-positive cancers unique characteristics respond well modern therapies. Researchers investigating biomarkers circulating HPV DNA immunoglobulin J polypeptide expression, which could provide valuable insights disease progression pave way for targeted effective strategies. In addition, use existing medications, fenofibrate, combat infections illustrates resourcefulness repurposing specific Challenges persist, need reliable early detection monitoring. Deeper viral-host interactions shape immunotherapy strategies that revolutionize approaches. Collaborative efforts between researchers, healthcare providers, policymakers play vital role translating substantial clinical benefits, improving quality life individuals affected diseases. HPV-associated HNCs present significant challenges, continuous research innovative offer hope brighter future combating growing epidemic patient care.
Language: Английский
Citations
0
Pathogens, Journal Year: 2025, Volume and Issue: 14(3), P. 293 - 293
Published: March 18, 2025
The human microbiome plays a vital role in maintaining homeostasis, acting as key regulator of host immunity and defense mechanisms. However, dysbiotic microbial communities may cause disruption the symbiotic relationship between local microbiota, leading to pathogenesis various diseases, including viral infections cancers. One most common infectious agents causing cancer is papilloma virus (HPV), which accounts for more than 90% cervical In cases, immune system activated clears HPV, whereas some infection persists can lead precancerous lesions. Over last two decades, advent next-generation sequencing (NGS) technology bioinformatics has allowed thorough in-depth analysis composition anatomical niches, allowing researchers unveil interactions underlying mechanisms through microbiota could affect HPV establishment, persistence, progression. Accordingly, present narrative review aims shed light on our understanding context its progression, mainly cancer. Furthermore, we explore by balance exert potential pathogenic or protective effects, either persistence disease outcomes clearance. Special interest given how modulate infection. Lastly, summarize latest findings therapeutic efficacy probiotics prebiotics preventing and/or treating vaginal transplantation while highlighting significance personalized medicine approaches emerging from NGS-based profiling artificial intelligence (AI) optimal management HPV-related diseases.
Language: Английский
Citations
0
Cancer Biomarkers, Journal Year: 2025, Volume and Issue: 42(1)
Published: Jan. 1, 2025
Background Oropharyngeal cancer rates continue to rise with no effective screening method. Persistent oral oncogenic human papillomavirus (HPV), antibodies HPV16 early (E) oncoproteins, and circulating tumor HPV DNA (ctHPVDNA) are biomarkers that show promise for use in HPV-related screening. Objective To assess the prevalence of their agreement middle-aged men. Methods Men aged 50–64 years from general population provided rinse blood samples as well information about demographics, tobacco/alcohol exposure, sexual behavior, disease history. Oral was tested plasma E ctHPVDNA using a droplet digital PCR (ddPCR)-based assay measures tissue modified viral (TTMV)-HPV (NavDx, Naveris, Inc.). We calculated frequency distributions variables interest between biomarkers. Results enrolled 1045 subjects April 2017 2024. The 954 results all three were included analysis. 4.9% DNA, 0.7% antibodies, 0.5% TTMV-HPV DNA. Conclusions low shows potential identify high-risk individuals eligible further clinical
Language: Английский
Citations
0
Seminars in Radiation Oncology, Journal Year: 2021, Volume and Issue: 31(4), P. 297 - 308
Published: Aug. 26, 2021
Language: Английский
Citations
24
Clinical Chemistry, Journal Year: 2024, Volume and Issue: 70(1), P. 285 - 296
Published: Jan. 1, 2024
Abstract Background More than 95% of cervical cancers and their precancerous lesions are caused by human papillomavirus (HPV). Cell-free (cf) HPV DNA detection in blood samples may serve as a monitoring tool for cancer. Methods In our methodological study, an panel simultaneous 24 types using mass spectrometry-based analysis was developed liquid biopsy approaches tested on positive cell lines, plasmid controls, high-grade squamous intraepithelial (HSIL) smear (n = 52). It validated cfDNA 40) cancer patients. Results The showed proficient results lines viral plasmids with limit 1 IU (international units)/µL HPV16/18 10GE/µL HPV11/31/33/39/45/51/52/58/59 specificity 100% the types. samples, detected sensitivity 98.14%. overall agreement between new clinical records 97.2% (κ 0.84). cfDNA, 26/40 (65.0%) any type, most infections due to hrHPV (24/26). were found all FIGO stages, highest positivity ratio III IV. Even lowest stage, I, had 12/23 (52.2%) patients plasma status. Conclusions This proof-of-concept paper shows that described assay produces reliable detecting multiplex high both cohorts. potential biopsy-based applications progression.
Language: Английский
Citations
3
BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)
Published: March 22, 2024
Cervicovaginal microbiome plays an important role in the persistence of HPV infection and subsequent disease development. However, cervicovaginal microbiota varied cross populations with different habits regions. Identification population-specific biomarkers from host metabolome axis may support early detection or surveillance HPV-induced cervical at all sites. Therefore, present study, to identify HPV-specific biomarkers, secretion serum samples HPV-infected patients (HPV group, n = 25) normal controls (normal 17) Xichang, China were collected for (16S rRNA gene sequencing) (UHPLC-MS/MS) analysis, respectively.
Language: Английский
Citations
3
Journal of Medical Virology, Journal Year: 2022, Volume and Issue: 95(1)
Published: June 1, 2022
We reviewed the literature on importance of selected anti-high-risk human papillomavirus (HR-HPV) antibodies (namely, 16/18 and early oncoproteins E6 E7) as potential serological markers for detection individuals at high risk cervical cancer. searched studies in PubMed Embase databases published from 2010 to 2020 against HR-HPV E7 proteins Pooled sensitivity specificity HPV16 HPV18 were calculated using a bivariate hierarchical random-effects model. A total 69 articles identified; we included three with 1550 participants. For HPV16/18 antibody tests, enzyme-linked immunosorbent assay-based assays had 18% detecting CIN2+ (95% confidence interval [CI]: 15-21) 96% CI: 92-98), slot-blot, was 28.9% 23.3-35.1) 72% 66.6-77.0) CIN2+, multiplex HPV serology assay based glutathione S-transferase, 16% 8.45-28.6) 98% 97-99) invasive HR-HPV16/18 showed specificity, but suboptimal cancer either population screening settings or point-of-care tests.
Language: Английский
Citations
14
Frontiers in Oncology, Journal Year: 2024, Volume and Issue: 13
Published: Jan. 3, 2024
Background The effect of chemoradiation on the anti-cancer immune response is being increasingly acknowledged; however, its clinical implications in treatment responses are yet to be fully understood. Human papillomavirus (HPV)-driven malignancies express viral oncogenic proteins which may serve as tumor-specific antigens and represent ideal candidates for monitoring peripheral T-cell receptor (TCR) changes secondary chemoradiotherapy (CRT). Methods We performed intra-tumoral pre- post-treatment TCR sequencing a cohort patients with locally-advanced HPV16-positive cancers treated CRT. An silico computational pipeline was used cluster repertoire based epitope-specificity predict affinity between these clusters HPV16-derived epitopes. Results Intra-tumoral diversity, CDR3β similarity clustering were predictive response. In responders, CRT triggered an increase clonality clonal relatedness. Post-treatment expansion baseline dominant TCRs associated Responders showed more clustered structures maintained displayed significantly structures. When applying by TCR-specificity methods, responders higher proportion predicted recognise HPV16 peptides. Conclusions Baseline characteristics clones outcome. Maintenance boosting pre-existing clonotypes key elements effective driven CRT, supporting paradigm system plays central role success current standard-of-care protocols.
Language: Английский
Citations
2
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(6), P. 3437 - 3437
Published: March 19, 2024
Elevated rates of human papillomavirus (HPV)-related anal high-grade squamous intraepithelial lesions (HSIL) and cancer (AC) in populations like men who have sex with (MSM) living HIV underscore the need for effective screening. While high-resolution anoscopy-guided biopsy is gold standard, limited provider availability poses a challenge. This has spurred interest identifying biomarkers improved AC prevention. Antibodies against HPV16 oncoprotein E6, known as markers cervical oropharyngeal cancers, are focus current study. The systematic review meta-analysis included six studies meeting inclusion criteria, assessing E6 seroprevalence individuals HSIL or AC. A two-step estimated pooled odds ratios 95% confidence intervals (CI) Pooled prevalence, sensitivity, specificity, diagnostic were also calculated. revealed 3.6-fold increased risk seropositive individuals, escalating to 26.1-fold increase specificity sensitivity indicated high (0.99; 95%CI: 0.99, 0.99) but lower (0.19; 0.10, 0.34) serostatus an biomarker. In conclusion, while demonstrates potential biomarker HPV-related AC, its utility standalone screening tool may be limited. Instead, it could serve effectively confirmation test, particularly high-risk populations, alongside other methods. Further research imperative explore seroconversion dynamics alternative algorithms.
Language: Английский
Citations
2