Single-Cell RNA Sequencing in Ovarian Cancer: Current Progress and Future Prospects

Progress in Biophysics and Molecular Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Utility of SOX17 Immunohistochemical Stain in Serous Fluid Cytology Cell Block Specimens

Diagnostic Cytopathology, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

ABSTRACT Background The identification of metastatic tumors in serous fluid cytology specimens (SFCS) has always been a challenge. In this study, we explored SOX17 as an immunohistochemical (IHC) marker for the diagnosis gynecologic body specimens. Methods We selected 97 tumor cases, including 85 SFCS with adequate cell block material (from primary, n = 30 and others, 55) 12 histology (thymic thyroid tumors). IHC was performed on all results were interpreted positive or negative. Positive further characterized by intensity (nuclear staining) weak (1+), moderate (2+), strong (3+) percentage cells focal (< 10%), patchy (10%–50%) diffuse (> 50%). Results primary tumors, exhibited nuclear staining 28 out two cases showing staining. All non‐gynecologic effusion SOX17‐negative except one case renal carcinoma, which displayed pattern. consisting thymic negative SOX17. Conclusions tract origin positive, while other case. This finding suggests that is excellent addition to panel working up at sites, specifically when are differential diagnosis.

Language: Английский

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Clinicopathological Study of 34 Cases of Clear Cell Adenocarcinoma of the Urinary Tract and Diagnostic Value of SOX 17 in its Differential Diagnosis

Human Pathology, Journal Year: 2025, Volume and Issue: unknown, P. 105767 - 105767

Published: April 1, 2025

Language: Английский

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SOX17 expression in mesonephric‐like adenocarcinomas and mesonephric remnants/hyperplasia of the female genital tract: Expanding its utility as a Müllerian biomarker

Histopathology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 8, 2024

Aims Recently, SOX17 has emerged as a promising biomarker for non‐mucinous Müllerian (ovarian and endometrial) carcinomas, demonstrating increased specificity in comparison to PAX8 while maintaining similar sensitivity. However, expression of mesonephric‐like adenocarcinoma (MLA), carcinoma the female genital tract with uncertain, but probably histogenesis, remains unexplored. This study aims address this gap. Methods results immunohistochemistry was performed on whole tissue sections from 68 MLAs originating endometrium or ovary seven cervical mesonephric well six remnants/hyperplasias. Using four‐tiered scoring system based distribution intensity staining, 68% MLA displayed negative/low (< 10%) pattern, which contrasts high observed most carcinomas. 22% demonstrated expression, other endometrial ovarian Similarly, five (72%) carcinomas cervix were SOX17‐negative, two cases (28%) positive. All remnants/hyperplasias negative. Conclusions The majority are negative exhibit low contrast diffuse strong commonly seen types carcinoma. subset demonstrate expression. Therefore, absence staining is supportive when differential includes another may also be useful differentiating malignancies benign glandular lesions.

Language: Английский

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ZBTB7A regulates LncRNA HOTAIR-mediated ELAVL1/SOX17 axis to inhibit malignancy and angiogenesis in endometrial carcinoma

Journal of Cancer Research and Clinical Oncology, Journal Year: 2024, Volume and Issue: 150(7)

Published: July 9, 2024

Abstract Background Endometrial cancer (EC) is the sixth most frequent in women worldwide and has higher fatality rates. The pathophysiology of EC complex, there are currently no reliable methods for diagnosing treating condition. Long non-coding RNA (lncRNA), according to mounting evidence, vital EC. HOTAIR regarded as a significant prognostic indicator ZBTB7A decreased proliferation migration, recent studies, however underlying mechanism still needs be clarified. Methods research utilized RT-qPCR measure expression clinical tissues various cell lines. Kaplan-Meier survival analysis was employed correlate levels with patient prognosis. Additionally, study examined interaction between using bioinformatics tools ChIP assays. experimental approach also involved manipulating lines assessing impact on cellular processes gene expression. Results found significantly compared adjacent normal tissues, high correlating poorer rates advanced characteristics. like HEC-1 A KLE showed cells. Knockdown these reduced proliferation, angiogenesis, migration. inversely correlated HOTAIR, its overexpression led decrease reduction malignant behaviors. uncovered that interacts ELAVL1 regulate SOX17, which turn activates Wnt/β-catenin pathway, promoting behaviors Conclusion critical regulator EC, contributing tumor growth poor Its regulation SOX17 via pathway underlines potential therapeutic target.

Language: Английский

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Identifying mesonephric‐like adenocarcinoma of the endometrium by combining SOX17 and PAX8 immunohistochemistry

Histopathology, Journal Year: 2024, Volume and Issue: 86(2), P. 268 - 277

Published: Sept. 4, 2024

Aims Mesonephric‐like adenocarcinoma (MLA) of the endometrium or ovary is a rare but distinct endometrial carcinoma which has combination characteristic morphological, immunohistochemical (IHC) and molecular features. SOX17 been recently identified as highly sensitive specific marker for ovarian carcinomas. In this study, we aimed to investigate expression in MLA together with other IHCs differentiate MLAs from Methods Seventeen previously diagnosed endometrial/ovarian were collected, multiple performed. Additionally, performed SOX17, PAX8 ER on tissue microarrays (TMAs) composed 652 carcinomas 2012 2015 when diagnostic criteria not established. Results All 17 showed diffuse strong positive PAX8, negative variable TTF1/GATA3 staining. Notably, all ( n = 10) focal weak/moderate 7) staining more stronger than subtypes. This finding prompted us screen TMAs before by an approach combined IHCs, 14 cases negative/focal weak identified. We further studied examining morphology performing additional (TTF1, GATA3, CD10) would classify seven (50%) them based morphological features CD10, TTF1 and/or GATA3 Conclusion Our results suggest that aid diagnosing if show SOX17.

Language: Английский

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Trichorhinophalangeal syndrome 1 expression in breast and nonbreast metastases from Müllerian, lung, gastrointestinal tract, and pancreatic primary tumors by immunohistochemistry with cytology cell block specimens

Cancer Cytopathology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 12, 2024

Abstract Background Trichorhinophalangeal syndrome 1 (TRPS1) expression in primary breast and other solid tumors has been investigated but its role as a marker metastatic is unclear. The objective of this study was to evaluate the sensitivity specificity TRPS1 cancer immunomarker that originated from breast, Müllerian, lung, gastrointestinal (GI), pancreatic with cell blocks fine‐needle aspiration (FNA) effusion specimens. Methods Cell were immunostained anti‐TRPS1 monoclonal antibody (clone EPR16171). Histochemical scores (H scores) (proportion × intensity; range, 0–300) assigned; H ≥10 considered positive. Overall, 160 specimens examined, including 127 FNAs (35 25 36 31 GI carcinomas) 33 (18 12 one two carcinomas). Results positive 51 53 (96%) carcinomas 28 107 (26.2%) nonbreast tumors. Metastatic carcinoma showed highest mean score 247.35, compared 45.36 8.4 5.88 for identifying origin 96.22% 72.89%, respectively. Conclusions Despite high overall sensitivity, lower because significantly than It important recognize broad range avoid an incorrect determination tumor’s organ origin.

Language: Английский

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Utility of immunocytochemistry in diagnosing abdominopelvic washings from patients undergoing radical surgery for endometrial cancer

CytoJournal, Journal Year: 2024, Volume and Issue: 21, P. 20 - 20

Published: June 25, 2024

Objective: This study aimed to explore the efficacy of immunocytochemistry in diagnosing abdominopelvic washings (APWs) and evaluate superiority cytology combined with over alone. Material Methods: Data on APW available cell blocks from patients who underwent radical surgery for endometrial cancer between January 2021 December 2022 were reviewed. Cytology was re-evaluated according a five-tier system. Immunocytochemistry analysis targets such as Sry box transcription factor 1(SOX17), Paired gene 2 (Pax-2) protein, Phosphatase tensin (PTEN), β-catenin performed each case non-negative cytology. Mismatch repair (MMR) protein P53 analyses using cases abnormal MMR or expression their primary lesion. The accuracies alone calculated. Results: Overall, 126 included this study, 18 whom demonstrated APW. Cell successfully prepared 16 cases. SOX17 positivity observed cases, including 1 serous carcinoma, clear 14 endometrioid carcinoma (EC). Loss Pax-2 PTEN APWs EC. deficiency noted two EC, mutation another Compared 10 metastatic carcinomas (10/18, 55.56%) diagnosed by alone, 15 malignant (15/18, 83.33%) confirmed through combination immunocytochemistry. more likely be than half myometrial invasion those no less ( P = 0.0067). probability occurrence slightly elevated EC exhibiting microcystic, elongated, fragmented(MELF) infiltrative growth 0.039). Conclusion: is useful Müllerian marker distinguishing epithelium offers evidence carcinoma. Furthermore, positive retained molecular features similar lesions. use multiple immunocytochemical markers can effectively enhance diagnostic efficiency APWs.

Language: Английский

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A comprehensive analysis of SOX17 expression by immunohistochemistry in human epithelial tumors, with an emphasis on gynecologic tumors

American Journal of Clinical Pathology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Abstract Objectives The objective of this study was to evaluate SOX17, a transcription factor from the Sry high-mobility group–related box superfamily, as diagnostic marker determine site origin using both whole-tissue sections and tissue microarrays (TMAs). Methods SOX17 immunohistochemistry performed on gynecologic nongynecologic tissues (N = 1004) internally constructed commercially available TMAs. nuclear reactivity scored positive or negative semiquantitative H score method Results Using TMAs, in 94% (n 155) endometrial tumors 96% 242) ovarian tumors. All breast cases 241) vulvar/cervical squamous cell carcinomas 150) were negative. Among 1004 20 sites, only organs with ovary, uterus, testis. Conclusions is sensitive specific for tested may be valuable adjunct PAX8 other commonly used markers confirm origin. expression lower mucinous tumors, endocervical adenocarcinoma, high-grade neuroendocrine undifferentiated/dedifferentiated carcinoma.

Language: Английский

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SOX17 Expression in Mesotheliomas and Benign Mesothelial Proliferations: Implications for Differential Diagnosis With Gynecologic Carcinomas

International Journal of Gynecological Pathology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 31, 2024

SOX17 has recently emerged as a novel immunohistochemical marker for cancers of endometrial and ovarian origin with improved specificity compared the widely used Mullerian PAX8. However, evaluation in benign malignant peritoneal mesothelial proliferations remains limited, these may mimic gynecologic carcinomas, particularly on small biopsies. We evaluated PAX8 expression 20 lesions (5 adenomatoid tumors, 5 well-differentiated papillary 10 inclusion cysts) 16 epithelioid mesotheliomas. The 17 female 3 male patients ranged from to 80 yr (median: 56.5 yr), while 9 females 7 males mesothelioma 47 85 57.5 yr). was positive (25%) (2 2 (13%) mesotheliomas, stained 8 (40%) (1 tumor, 1 6 cysts), Results stains showed incomplete concordance, agreement 15 (75%) 14 (88%) Our findings suggest that positivity alone is insufficient confirm diagnosis carcinoma over proliferation pathologists should exercise caution when entities are diagnostic considerations.

Language: Английский

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