Double–Multiplex Immunostainings for Immune Profiling of Invasive Breast Carcinoma: Emerging Novel Immune-Based Biomarkers DOI Open Access
Sofia D. P. Theodorou, Konstantinos Ntostoglou, Ilias P. Nikas

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2838 - 2838

Published: March 21, 2025

The role of tumor microenvironment in invasive breast cancer prognosis and treatment is highly appreciated. With the advent immunotherapy, immunophenotypic characterization primary tumors gaining attention as it can improve patient stratification. Here, we discuss benefits spatial analysis employing double multiplex immunostaining, allowing simultaneous detection more than one protein on same tissue section, which turn helps us provide functional insight into infiltrating immune cells within tumors. We focus studies demonstrating prognostic predictive impact distinct tumor-infiltrating lymphocyte subpopulations including different CD8(+) T subsets well CD4(+) tumor-associated macrophages carcinoma. clinical value cell topography also further refer to how integration digital pathology artificial intelligence routine practice could enhance accuracy immunostainings evaluation microenvironment, maximizing our perception host response improvement turning decision-making towards precise immune-associated therapies.

Language: Английский

Advancements in Digital Cytopathology Since COVID-19: Insights from a Narrative Review of Review Articles DOI Open Access
Daniele Giansanti

Healthcare, Journal Year: 2025, Volume and Issue: 13(6), P. 657 - 657

Published: March 17, 2025

Background/Objectives: The integration of digitalization in cytopathology is an emerging field with transformative potential, aiming to enhance diagnostic precision and operational efficiency. This narrative review reviews (NRR) seeks identify prevailing themes, opportunities, challenges, recommendations related the process cytopathology. Methods: Utilizing a standardized checklist quality control procedures, this examines recent advancements future implications domain. Twenty-one studies were selected through systematic process. Results: results highlight key trends, digital First, study identifies pivotal themes that reflect ongoing technological transformation, guiding focus areas field. A major trend artificial intelligence (AI), which increasingly critical improving accuracy, streamlining workflows, assisting decision making. Notably, AI technologies like large language models (LLMs) chatbots are expected provide real-time support automate tasks, though concerns around ethics privacy must be addressed. also emphasize need for protocols, comprehensive training, rigorous validation ensure tools reliable effective across clinical settings. Lastly, holds significant potential improve healthcare accessibility, especially remote areas, by enabling faster, more efficient diagnoses fostering global collaboration telepathology. Conclusions: Overall, highlights impact cytopathology, efficiency, accessibility whole-slide imaging While plays role, broader on integrating solutions workflows collaboration. Addressing challenges such as standardization, ethical considerations crucial fully realize these advancements.

Language: Английский

Citations

0

Double–Multiplex Immunostainings for Immune Profiling of Invasive Breast Carcinoma: Emerging Novel Immune-Based Biomarkers DOI Open Access
Sofia D. P. Theodorou, Konstantinos Ntostoglou, Ilias P. Nikas

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 2838 - 2838

Published: March 21, 2025

The role of tumor microenvironment in invasive breast cancer prognosis and treatment is highly appreciated. With the advent immunotherapy, immunophenotypic characterization primary tumors gaining attention as it can improve patient stratification. Here, we discuss benefits spatial analysis employing double multiplex immunostaining, allowing simultaneous detection more than one protein on same tissue section, which turn helps us provide functional insight into infiltrating immune cells within tumors. We focus studies demonstrating prognostic predictive impact distinct tumor-infiltrating lymphocyte subpopulations including different CD8(+) T subsets well CD4(+) tumor-associated macrophages carcinoma. clinical value cell topography also further refer to how integration digital pathology artificial intelligence routine practice could enhance accuracy immunostainings evaluation microenvironment, maximizing our perception host response improvement turning decision-making towards precise immune-associated therapies.

Language: Английский

Citations

0