Post-injury Buprenorphine Administration Is Associated With Long-Term Region-Specific Glial Alterations in Rats DOI Open Access
Jane Ryu,

Pantea Jeizan,

Saira Ahmed

et al.

Published: Aug. 12, 2022

Traumatic brain injury (TBI) is a major leading cause of death and disability. While previous studies regarding focal pathologies following TBI have been done, there lack information concerning the role analgesics their influences on pathology. Buprenorphine (Bup), an opioid analgesic, commonly used analgesic in experimental models. Our investigated acute effects Buprenorphine-sustained release-Lab (Bup-SR-Lab) diffuse neuronal/glial pathology, neuroinflammation, cell damage, systemic physiology. The current study longer-term chronic outcomes Bup-SR-Lab treatment at 4 weeks utilizing central fluid percussion (cFPI) model adult male rats. Histological assessments physiological changes, neuronal cortical thalamic cytokine expression, microglial astrocyte morphological myelin alterations were as we had done our study. In Whisker Nuisance Task (WNT) was also performed pre- 4w post-injury to assess changes somatosensory sensitivity saline or treatment. no impact overall physiology damage regardless region injury, nor did it any significant sensitivity. However, greater IL-4 expression with observed compared treated animals. Microglia astrocytes demonstrated region-specific associated treatment, which microglia particularly vulnerable Bup-mediated changes. There discernable injury-specific differences integrity specific basic protein (MBP) isoform This indicates that use could TBI-induced glial manor injury.

Language: Английский

Astrocytes require perineuronal nets to maintain synaptic homeostasis in mice DOI Creative Commons
Bhanu P. Tewari, AnnaLin M. Woo, Courtney Prim

et al.

Nature Neuroscience, Journal Year: 2024, Volume and Issue: 27(8), P. 1475 - 1488

Published: July 17, 2024

Abstract Perineuronal nets (PNNs) are densely packed extracellular matrices that cover the cell body of fast-spiking inhibitory neurons. PNNs stabilize synapses inhibiting synaptic plasticity. Here we show terminals interneurons localize to holes in adult mouse somatosensory cortex. Approximately 95% contain and astrocytic processes expressing Kir4.1, glutamate GABA transporters. Hence, tripartite synapses. In brain, PNN degradation causes an expanded coverage neuronal somata without altering axon terminals. The loss impairs transmitter potassium uptake, resulting spillage into extrasynaptic space. Our data astrocytes cooperate synaptically released signals physiological conditions. Their combined action is altered models Alzheimer’s disease epilepsy where disrupted.

Language: Английский

Citations

23
A glial perspective on the extracellular matrix and perineuronal net remodeling in the central nervous system DOI Creative Commons
Bhanu P. Tewari, Lata Chaunsali, Courtney Prim

et al.

Frontiers in Cellular Neuroscience, Journal Year: 2022, Volume and Issue: 16

Published: Oct. 20, 2022

A structural scaffold embedding brain cells and vasculature is known as extracellular matrix (ECM). The physical appearance of ECM in the central nervous system (CNS) ranges from a diffused, homogeneous, amorphous, nearly omnipresent to highly organized distinct morphologies such basement membranes perineuronal nets (PNNs). changes its composition organization during development, adulthood, aging, several CNS pathologies. This spatiotemporal dynamic nature PNNs brings unique versatility their functions spanning neurogenesis, cell migration differentiation, axonal growth, pathfinding cues, etc., developing brain, stabilizing synapses, neuromodulation, being an active partner tetrapartite synapses adult brain. malleability governed by both intrinsic extrinsic factors. Glial are among major factors that facilitate remodeling PNN, thereby acting key regulators diverse PNN health diseases. In this review, we discuss recent advances our understanding how glial normal pathological states CNS.

Language: Английский

Citations

52
Diffusion MRS tracks distinct trajectories of neuronal development in the cerebellum and thalamus of rat neonates DOI Open Access
Clémence Ligneul, Lily R. Qiu, William T. Clarke

et al.

Published: April 22, 2025

It is currently impossible to non-invasively assess cerebellar cell structure during early development. Here we propose a novel approach and longitudinally track cell-specific development using diffusion-weighted magnetic resonance spectroscopy in combination with microstructural modelling. Tracking metabolite diffusion allows us probe developmental trajectories the cerebellum thalamus of healthy rat neonates from post-natal day (P) 5 P30. Additionally, by comparing different analytical biophysical models can follow differential contribution bodies neurites The serves as control region sensitivity our method differences between regions. We found significant thalamic metabolites properties. For most metabolites, signal attenuation stronger thalamus, suggesting less restricted compared cerebellum. There also trend for lower ADCs increasing age, restriction diffusion. This particularly striking taurine thalamus. use modelling interpret these differences. report decreased sphere fraction (or an increased neurite fraction) age total creatine cerebellum, marking dendritic growth. Surprisingly, U-shape segment length (the distance two embranchments tree) agreeing age-matching morphometry openly available 3D-Purkinje reconstructions. Results demonstrate that MRS probes neuronal non-invasively.

Language: Английский

Citations

0
Perineuronal nets support astrocytic ion and glutamate homeostasis at tripartite synapses DOI Creative Commons
Bhanu P. Tewari, AnnaLin M. Woo, Courtney Prim

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Feb. 2, 2023

Abstract Perineuronal nets (PNNs) are dense, negatively charged extracellular matrices that cover the cell body of fast-spiking inhibitory neurons. Synapses can be embedded and stabilized by PNNs believed to prevent synaptic plasticity. We find in cortical interneurons terminals localize perforations PNNs, 95% which contain either excitatory or synapses both. The majority also colocalize with astrocytic processes expressing Kir4.1 as well glutamate (Glu) GABA transporters, hence considered tripartite synapses. In adult brain, degradation does not alter axonal but causes expansion coverage neuronal somata. However, loss impairs transmitter K+ uptake spillage Glu into extrasynaptic space. This data suggests a hitherto unrecognized role synergize astrocytes synaptically released signals.

Language: Английский

Citations

8
Glia Regulate the Development, Function, and Plasticity of the Visual System From Retina to Cortex DOI Creative Commons
Nicholas J. Benfey, David Foubert, Edward S. Ruthazer

et al.

Frontiers in Neural Circuits, Journal Year: 2022, Volume and Issue: 16

Published: Feb. 1, 2022

Visual experience is mediated through a relay of finely-tuned neural circuits extending from the retina, to retinorecipient nuclei in midbrain and thalamus, cortex which work together translate light information entering our eyes into complex dynamic spatio-temporal representation world. While experience-dependent developmental refinement mature function neurons each major stage vertebrate visual system have been extensively characterized, contributions glial cells populating region are comparatively understudied despite important findings demonstrating that they mediate crucial processes related development, function, plasticity system. In this article we review mechanisms for neuron-glia communication throughout system, as well functional roles attributed astrocytes microglia development processing. We will also discuss aspects remain unclear, integrating knowns unknowns about glia advance new hypotheses guide future experimental work.

Language: Английский

Citations

11
Post-Injury Buprenorphine Administration Is Associated with Long-Term Region-Specific Glial Alterations in Rats DOI Creative Commons
Jane Ryu,

Pantea Jeizan,

Saira Ahmed

et al.

Pharmaceutics, Journal Year: 2022, Volume and Issue: 14(10), P. 2068 - 2068

Published: Sept. 28, 2022

Traumatic brain injury (TBI) is a major leading cause of death and disability. While previous studies regarding focal pathologies following TBI have been done, there lack information concerning the role analgesics their influences on pathology. Buprenorphine (Bup), an opioid analgesic, commonly used analgesic in experimental models. Our investigated acute effects Buprenorphine-sustained release-Lab (Bup-SR-Lab) diffuse neuronal/glial pathology, neuroinflammation, cell damage, systemic physiology. The current study longer-term chronic outcomes Bup-SR-Lab treatment at 4 weeks utilizing central fluid percussion (cFPI) model adult male rats. Histological assessments physiological changes, neuronal cortical thalamic cytokine expression, microglial astrocyte morphological myelin alterations were as we had done our study. In Whisker Nuisance Task (WNT) was also performed pre- 4w post-injury to assess changes somatosensory sensitivity saline or treatment. no impact overall physiology damage regardless region injury, nor did it any significant sensitivity. However, greater IL-4 expression with observed compared treated animals. Microglia astrocytes demonstrated region-specific associated treatment, which microglia particularly vulnerable Bup-mediated changes. There discernable injury-specific differences integrity specific basic protein (MBP) isoform This indicates that use could TBI-induced glial manner injury.

Language: Английский

Citations

6
Sonic hedgehog-dependent recruitment of GABAergic interneurons into the developing visual thalamus DOI Creative Commons
Rachana Deven Somaiya, Katelyn Stebbins, Ellen C. Gingrich

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: Nov. 7, 2022

Axons of retinal ganglion cells (RGCs) play critical roles in the development inhibitory circuits visual thalamus. We previously reported that RGC axons signal astrocytes to induce expression fibroblast growth factor 15 (FGF15), a motogen required for GABAergic interneuron migration into However, how thalamic generate Fgf15 and influence remains unknown. Here, we demonstrate impairing activity had little impact on recruitment mouse Instead, our data show retinal-derived sonic hedgehog (SHH) is essential recruitment. Specifically, thalamus-projecting RGCs express SHH downstream components signaling. Deletion RGC-derived leads significant decrease expression, as well percentage interneurons recruited Overall, findings identify morphogen-dependent neuron–astrocyte signaling mechanism interneurons.

Language: Английский

Citations

6
Sonic hedgehog-dependent recruitment of GABAergic interneurons into the developing visual thalamus DOI Open Access
Rachana Deven Somaiya, Katelyn Stebbins, Ellen C. Gingrich

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Feb. 22, 2022

Abstract Axons of retinal ganglion cells (RGCs) play critical roles in the development inhibitory circuits visual thalamus. We previously reported that RGC axons signal astrocytes to induce expression fibroblast growth factor 15 (FGF15), a motogen required for GABAergic interneuron migration into However, how thalamic generate Fgf15 and influence remains unknown. Here, we demonstrate impairing activity had little impact on recruitment mouse Instead, our data show retinal-derived sonic hedgehog (SHH) is essential recruitment. Specifically, thalamus-projecting RGCs express SHH downstream components signaling. Deletion RGC-derived leads significant decrease expression, as well percentage interneurons recruited Thus, findings identify morphogen-dependent neuron-astrocyte signaling mechanism interneurons.

Language: Английский

Citations

2
Post-injury Buprenorphine Administration Is Associated With Long-Term Region-Specific Glial Alterations in Rats DOI Open Access
Jane Ryu,

Pantea Jeizan,

Saira Ahmed

et al.

Published: Aug. 12, 2022

Traumatic brain injury (TBI) is a major leading cause of death and disability. While previous studies regarding focal pathologies following TBI have been done, there lack information concerning the role analgesics their influences on pathology. Buprenorphine (Bup), an opioid analgesic, commonly used analgesic in experimental models. Our investigated acute effects Buprenorphine-sustained release-Lab (Bup-SR-Lab) diffuse neuronal/glial pathology, neuroinflammation, cell damage, systemic physiology. The current study longer-term chronic outcomes Bup-SR-Lab treatment at 4 weeks utilizing central fluid percussion (cFPI) model adult male rats. Histological assessments physiological changes, neuronal cortical thalamic cytokine expression, microglial astrocyte morphological myelin alterations were as we had done our study. In Whisker Nuisance Task (WNT) was also performed pre- 4w post-injury to assess changes somatosensory sensitivity saline or treatment. no impact overall physiology damage regardless region injury, nor did it any significant sensitivity. However, greater IL-4 expression with observed compared treated animals. Microglia astrocytes demonstrated region-specific associated treatment, which microglia particularly vulnerable Bup-mediated changes. There discernable injury-specific differences integrity specific basic protein (MBP) isoform This indicates that use could TBI-induced glial manor injury.

Language: Английский

Citations

2