A consensus definition for deep layer 6 excitatory neurons in mouse neocortex DOI Creative Commons
Su-Jeong Kim, Travis A. Babola, Kihwan Lee

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

To understand neocortical function, we must first define its cell types. Recent studies indicate that neurons in the deepest cortical layer play roles mediating thalamocortical interactions and modulating brain state are implicated neuropsychiatric disease. However, understanding functions of deep 6 (L6b) has been hampered by lack agreed upon definitions for these We compared commonly used methods defining L6b neurons, including molecular, transcriptional morphological approaches as well transgenic mouse lines, identified a core population neurons. This does not innervate sensory thalamus, unlike corticothalamic (L6CThNs) more superficial 6. Rather, single project ipsilaterally between areas. Although undergo early developmental changes, found their intrinsic electrophysiological properties were stable after postnatal week. Our results provide consensus definition enabling comparisons across studies.

Language: Английский

Postnatal development of vasoactive intestinal polypeptide‐expressing GABAergic interneurons in mouse somatosensory cortex DOI Creative Commons

Clara A. Simacek,

Sergei Kirischuk, Thomas Mittmann

et al.

Acta Physiologica, Journal Year: 2025, Volume and Issue: 241(2)

Published: Jan. 13, 2025

Despite dysfunctional vasoactive intestinal polypeptide-positive interneurons (VIP-INs) being linked to the emergence of neurodevelopmental disorders, temporal profile VIP-IN functional maturation and cortical network integration remains unclear. Postnatal development was traced with patch clamp experiments in somatosensory cortex Vip-IRES-cre x tdTomato mice. Age groups were chosen during barrel field formation, before after activation main sensory inputs, adult animals (postnatal days (P) P3-4, P8-10, P14-16, P30-36). Changes passive active membrane properties show a towards accelerated signal integrations. Excitatory inhibitory postsynaptic currents (EPSCs IPSCs) showed progressive into networks, likely via synaptogenesis: mEPSC frequency increased while mIPSC at P14-16. Only kinetics became accelerated, E/I ratio synaptic defined as charge transfer, remained constant throughout investigated developmental stages. Evoked (e)EPSCs (e)IPSCs amplitudes, only eIPSCs demonstrated faster kinetics. eEPSCs revealed paired-pulse facilitation by indicating probably decrease presynaptic release probability (pr) depression adulthood. also latter, suggesting pr for both transmission pathways this time point. VIP-INs mature pursue different strategies avoid overexcitation. become stronger shorter pre- alterations, promoting execution whisking.

Language: Английский

Citations

1
A consensus definition for deep layer 6 excitatory neurons in mouse neocortex DOI Creative Commons
Su-Jeong Kim, Travis A. Babola, Kihwan Lee

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

To understand neocortical function, we must first define its cell types. Recent studies indicate that neurons in the deepest cortical layer play roles mediating thalamocortical interactions and modulating brain state are implicated neuropsychiatric disease. However, understanding functions of deep 6 (L6b) has been hampered by lack agreed upon definitions for these We compared commonly used methods defining L6b neurons, including molecular, transcriptional morphological approaches as well transgenic mouse lines, identified a core population neurons. This does not innervate sensory thalamus, unlike corticothalamic (L6CThNs) more superficial 6. Rather, single project ipsilaterally between areas. Although undergo early developmental changes, found their intrinsic electrophysiological properties were stable after postnatal week. Our results provide consensus definition enabling comparisons across studies.

Language: Английский

Citations

0