Clinical efficacy of conbercept combined with micropulse laser in patients with polypoidal choroidal vasculopathy DOI Creative Commons

Qingyan Liu,

Yao Lu, Shuo Sun

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 28, 2024

Abstract Objective To evaluate the clinical efficacy of conbercept combined with micropulse laser(MPL) in treating polypoidal choroidal vasculopathy (PCV). Methods In this prospective, randomized controlled trial conducted from February 2023 to April 2024, 52 patients (52eyes) PCV at ### hospital were enrolled. Participants into a monotherapy group (27cases, 27eyes) and combination treatment (conbercept plus laser(MPL); 25cases, 25eyes). After an initial series three intravitreal injections, all followed Treat & Extend (T&E) protocol. Aqueous humor samples collected before each first cytokines levels measured after sample collection. The received 577nm MPL application two weeks following injection. best corrected visual acuity (BCVA) 、central macular thickness (CMT) total volume (TMV) assessed monthly for months. Results From third month post-injection, both groups showed improvements BCVA reductions CMT TMV, demonstrating significantly greater evaluation (P < 0.05). Additionally, aqueous cytokines, including VEGF-A, IL-5, MCP-1, Ang-2, reduced groups. Reductions VEGF-A MCP-1 concentrations positively correlated BCVA, CMT, TMV 0.01). Conclusion Conbercept therapy demonstrates superior compared injection alone PCV. Furthermore, changes may serve as predictive markers retinal morphology these patients.

Language: Английский

Circulating miRNAs and Preeclampsia: From Implantation to Epigenetics DOI Open Access
Stefano Raffaele Giannubilo, Monia Cecati, Daniela Marzioni

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(3), P. 1418 - 1418

Published: Jan. 24, 2024

In this review, we comprehensively present the literature on circulating microRNAs (miRNAs) associated with preeclampsia, a pregnancy-specific disease considered primary reason for maternal and fetal mortality morbidity. miRNAs are single-stranded non-coding RNAs, 20–24 nt long, which control mRNA expression. Changes in miRNA expression can induce variation relative level influence cellular homeostasis, strong presence of all body fluids has made them useful biomarkers several diseases. Preeclampsia is multifactorial disease, but etiopathogenesis remains unclear. The functions trophoblasts, including differentiation, proliferation, migration, invasion apoptosis, essential successful pregnancy. During early stages placental development, trophoblasts strictly regulated by molecular pathways; however, an imbalance these pathways lead to severe lesions pregnancy complications. We then discuss role trophoblast pathogenesis, diagnosis prediction preeclampsia. also potential from epigenetic perspective possible future therapeutic implications.

Language: Английский

Citations

9
Role of CD4+ T cell-derived cytokines in the pathogenesis of uveitis DOI Creative Commons
Tingting Meng, Lili Nie, Ying Wang

et al.

Clinical and Experimental Medicine, Journal Year: 2025, Volume and Issue: 25(1)

Published: Feb. 5, 2025

Uveitis refers to a diverse group of inflammatory diseases that affecting the uveal tract, comprising iris, ciliary body, and choroid, with potential repercussions ranging from visual impairment blindness. The role autoimmunity in uveitis etiology is complex still under investigation. CD4+ T cells intricately regulate immune responses through their subtypes: Th1, Th2, Th17, Treg (T regulatory), Tfh (follicular helper) cells. Each cell subtype secretes specific cytokines either pathogenic or protective implications uveitis. Th1 cells, characterized by IFN-γ secretion T-bet expression, drive type 1 against intracellular pathogens. Conversely, Th2 which produce interleukin (IL)-4, IL-5, IL-13 express transcription factor GATA3, mediate 2 larger extracellular threats like helminths. Th17 generating IL-17 IL-22 controlled RORγt, engage 3 select releasing IL-21 governed Bcl6, aid B antibody production. Tregs, identified Foxp3, exert regulatory functions homeostasis. This review delves into roles cell-derived uveitis, emphasizing intricate involvement disease progression resolution. Insight these mechanisms might guide therapeutic approaches targeting management.

Language: Английский

Citations

1
Microglial mediators in autoimmune Uveitis: Bridging neuroprotection and neurotoxicity DOI
Monika Sharma, Pankaj Pal, Sukesh Kumar Gupta

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 136, P. 112309 - 112309

Published: May 29, 2024

Language: Английский

Citations

7
Anti-Inflammatory Effects of Clarstatin, a Shared-Epitope–Antagonistic Cyclic Peptide, on Experimental Autoimmune Uveitis in Mice DOI Creative Commons

Shira Merzbach,

Adi Schumacher‐Klinger,

Michal Klazas

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2025, Volume and Issue: 66(1), P. 13 - 13

Published: Jan. 7, 2025

Polymorphism and mutations of human leukocyte antigens (HLAs) calreticulin are risk factors for uveitis. Here, we sought to determine the therapeutic effects Clarstatin, a cyclic peptide antagonist HLA shared-epitope-calreticulin interaction, in experimental autoimmune uveitis (EAU) models. Mice were injected with Clarstatin intraperitoneally its effect was compared that corticosteroid. EAU evaluated clinically histologically. Ocular infiltration CD45+ hematopoietic cells splenocyte CD4+ expression determined using immunofluorescence flow cytometry (fluorescence-activated cell sorting [FACS]). ELISA used measure ocular level proinflammatory cytokines. significantly ameliorated severity C57BL/6J mild B10.RIII severe mice There significant dose time-dependent decrease, range 30% 80%, clinical score (P < 0.05), histological number retinal spleen 0.05 P 0.001, respectively), comparable reduced intraocular levels interleukin 6 (IL-6; 0.05) monocyte chemoattractant protein-1 (MCP-1; 0.01) by 41% 59%, respectively. Systemic delivery improved EAU. Its potential anti-inflammatory represent novel mode treatment inflammation. It may also be relevant modality systemic conditions which plays role their pathogenesis.

Language: Английский

Citations

0
Interferons and Toxoplasma shape PD-L1 regulation in retinal barrier cells: The critical role of proteases DOI Creative Commons
Benjamin Geiller, Cintia Cevallos,

Iuliia Tsybenko

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract The apicomplexan parasite Toxoplasma gondii establishes chronic infection in the central nervous system, including retina, causing ocular toxoplasmosis (OT). This persistence relies on a fine balance between inflammatory and immunomodulatory mechanisms, especially immune-privileged environment. We previously described immunologic interactions retinal cells, particularly roles of type I III interferons. In this study, we investigated regulatory dynamics PD-L1, receptor immune cells. first mechanisms PD-L1 regulation interferons an in vitro T. gondiiinfection model using mono- co-culture systems human microglia, astrocytes, Müller also assessed expression outer blood-retina barrier (oBRB) differentiated pigmented epithelial (RPE) Additionally, looked at cell activation, these mouse OT. Our findings reveal new for regulating glial activation RPE, Müller, astrocytes microglial Notably, most abundant cells showed highest baseline both mRNA protein levels, responded robustly to interferon stimulation. points more prominent immunoregulatory role retina than recognized. Furthermore, identified protease-dependent mechanism that reduces our vitrooBRB potentially contributing evasion inflammation during Finally, murine OT, demonstrated reached its peak day 7 post-infection neutralization plays crucial activation. orchestrates IFN dependent interaction downregulates oBRB by mechanism, infection. results can pave way fully elucidate networks offering potential targets therapeutic interventions OT other diseases.

Language: Английский

Citations

0
Ocular decompression retinopathy after anterior chamber paracentesis - case series and systematic review DOI Creative Commons
Shu-Fen Hsu, Yung-Jen Lai,

Lee-Jen Chen

et al.

Taiwan Journal of Ophthalmology, Journal Year: 2025, Volume and Issue: unknown

Published: March 13, 2025

Abstract To elucidate the mechanisms underlying multilayered retinal hemorrhage following anterior chamber paracentesis and to provide a comprehensive overview of clinical features, risk factors, prognosis for this complication. We conducted systematic review in accordance with Preferred Reporting Items Systematic Reviews Meta-Analyses guidelines. A literature search was carried out up March 1, 2024, across PubMed/MEDLINE, Cochrane Library, EMBASE databases. Studies reporting cases were included. In addition, we present three that developed postparacentesis, diagnoses uveitic glaucoma, neovascular endophthalmitis. reviewed on seven patients, addition from our institution, who paracentesis. Uveitis most prevalent diagnosis, accounting four cases. Other included macular branch arterial occlusion, primary open-angle glaucoma. Fundus examinations revealed preretinal six superficial three, blot nine, Roth spots seven. The overall favorable, none patients requiring additional interventions ocular decompression retinopathy (ODR). reliability is constrained by limited number available studies inherent biases case reports. Nevertheless, evidence suggests can cause ODR. ODR mimic central vein occlusion fundus images, making accurate diagnosis essential, as therapeutic approaches these two conditions differ significantly.

Language: Английский

Citations

0
Müller cells trophism and pathology as the next therapeutic targets for retinal diseases. DOI Creative Commons
Alessandro Arrigo, Ottavio Cremona, Emanuela Aragona

et al.

Progress in Retinal and Eye Research, Journal Year: 2025, Volume and Issue: unknown, P. 101357 - 101357

Published: April 1, 2025

Language: Английский

Citations

0
Immune Factors, Immune Cells and Inflammatory Diseases DOI Open Access
Alister C. Ward

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(4), P. 2417 - 2417

Published: Feb. 19, 2024

The immune system comprises distinct innate and adaptive arms, each of which contains many layers to provide a coordinated, sequential response insults [...].

Language: Английский

Citations

1
Integrating pathogen- and host-derived blood biomarkers for enhanced tuberculosis diagnosis: a comprehensive review DOI Creative Commons

Zhaodong Li,

Yunlong Hu,

Wenfei Wang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 9, 2024

This review explores the evolving landscape of blood biomarkers in diagnosis tuberculosis (TB), focusing on derived both from pathogen and host. These provide critical insights that can improve diagnostic accuracy timeliness, essential for effective TB management. The document highlights recent advancements molecular techniques have enhanced detection characterization specific biomarkers. It also discusses integration these into clinical practice, emphasizing their potential to revolutionize diagnostics by enabling more precise monitoring disease progression. Challenges such as variability biomarker expression need standardized validation processes are addressed ensure reliability across different populations settings. calls further research refine fully harness fight against TB, suggesting a multidisciplinary approach overcome existing barriers optimize strategies. comprehensive analysis underscores significance invaluable tools global effort control eliminate TB.

Language: Английский

Citations

1
Case report: Longitudinal evaluation and treatment of a melanoma-associated retinopathy patient DOI Creative Commons
Ryan M Mosavi-Hecht,

Paul Yang,

Barrett Heyer

et al.

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Sept. 10, 2024

Melanoma-associated retinopathy (MAR) is a paraneoplastic syndrome associated with cutaneous metastatic melanoma in which patients develop vision deficits that include reduced night vision, poor contrast sensitivity, and photopsia. MAR caused by autoantibodies targeting TRPM1, an ion channel found melanocytes retinal ON-bipolar cells (ON-BCs). The visual symptoms arise when TRPM1 enter ON-BCs block the function of thus detection patient serum key criterion diagnosing MAR. Electroretinograms are used to measure impact on ON-BC represent another important diagnostic tool for To date, case reports have included one or both components, but only single time point course patient's disease. Here, we report supported longitudinal analysis autoantibody detection, function, ocular inflammation, vascular integrity, response slow-release intraocular corticosteroids. Integrating these data oncological ophthalmological records reveals novel insights regarding pathogenesis, progression, treatment, may inform new research expand our collective understanding In brief, find can disrupt even levels barely detectable western blot immunohistochemistry; dexamethasone treatment alleviates despite high circulating autoantibodies, implicating antibody access retina as factor pathogenesis. Elevated inflammatory cytokine eyes be responsible observed damage blood-retinal barrier subsequent entry into retina.

Language: Английский

Citations

1