Breaking the mold: 3D cell cultures reshaping the future of cancer research DOI Creative Commons
Sandra Cordeiro, Beatriz B P P Oliveira, Rúben Valente

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 26, 2024

Despite extensive efforts to unravel tumor behavior and develop anticancer therapies, most treatments fail when advanced clinical trials. The main challenge in cancer research has been the absence of predictive models, accurately mimicking tumoral processes response treatments. microenvironment (TME) shows several human-specific physical chemical properties, which cannot be fully recapitulated by conventional 2D cell cultures or

Language: Английский

Assembloid models of cell-cell interaction to study tissue and disease biology DOI
Massimo M. Onesto, Ji‐il Kim, Sergiu P. Paşca

et al.

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(11), P. 1563 - 1573

Published: Oct. 24, 2024

Language: Английский

Citations

10
Anoikis in cell fate, physiopathology, and therapeutic interventions DOI Creative Commons
Jie Mei,

Xue‐Yao Jiang,

Huixiang Tian

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(10)

Published: Sept. 15, 2024

The extracellular matrix (ECM) governs a wide spectrum of cellular fate processes, with particular emphasis on anoikis, an integrin-dependent form cell death. Currently, anoikis is defined as intrinsic apoptosis. In contrast to traditional apoptosis and necroptosis, integrin correlates ECM signaling intracellular cascades, describing the full process anoikis. However, frequently overlooked in physiological pathological processes well vitro research models. this review, we summarized role spanning embryonic development, organ tissue repair, inflammatory responses, cardiovascular diseases, tumor metastasis, so on. Similarly, realm stem focused functional evolution cells, offers potential solution various challenges, including culture models, therapy, transplantation, engineering applications, which are largely based regulation by More importantly, regulatory mechanisms molecular will provide new strategies for therapeutic interventions (drug therapy cell-based therapy) disease. summary, review provides systematic elaboration thus shedding light its future research.

Language: Английский

Citations

9
Tumour organoids and assembloids: Patient‐derived cancer avatars for immunotherapy DOI Creative Commons
Jie Mei, Xingjian Liu, Huixiang Tian

et al.

Clinical and Translational Medicine, Journal Year: 2024, Volume and Issue: 14(4)

Published: April 1, 2024

Abstract Background Organoid technology is an emerging and rapidly growing field that shows promise in studying organ development screening therapeutic regimens. Although organoids have been proposed for a decade, concerns exist, including batch‐to‐batch variations, lack of the native microenvironment clinical applicability. Main body The concept has derived patient‐derived tumour (PDTOs) personalized drug new discovery, mitigating risks medication misuse. greater similarity between PDTOs primary tumours, more influential model will be. Recently, ‘tumour assembloids’ inspired by cell‐coculture attracted attention to complement current PDTO technology. High‐quality must reassemble critical components, multiple cell types, matrix, paracrine factors, angiogenesis microorganisms. This review begins with brief overview history PDTOs, followed approaches generating assembloids. Personalized practised; however, it remains unclear whether can predict immunotherapies, immune drugs (e.g. checkpoint inhibitors) cells tumour‐infiltrating lymphocyte, T receptor‐engineered chimeric antigen receptor‐T cell). as cancer avatars patients, be expanded stored form biobank. Conclusion Fundamental research trials are ongoing, intention use these models replace animals. Pre‐clinical immunotherapy using beneficial patients. Key Points not yet constructed key cellular non‐cellular components. should expandable editable. promising preclinical unless mature established. biobanks consensual standards urgently needed.

Language: Английский

Citations

8
Patient-Derived Organoid Models for NKT Cell-Based Cancer Immunotherapy DOI Open Access
Pablo A. Palacios,

Iván Flores,

Lucas Cereceda

et al.

Cancers, Journal Year: 2025, Volume and Issue: 17(3), P. 406 - 406

Published: Jan. 26, 2025

Invariant Natural Killer T (iNKT) cells are a unique subset of that bridge innate and adaptive immunity, displaying potent anti-tumor properties through cytokine secretion, direct cytotoxicity, recruitment immune effector such as CD8+ NK cells. Despite their therapeutic potential, the immunosuppressive tumor microenvironment (TME), characterized by regulatory cells, myeloid-derived suppressor (MDSCs), tumor-associated macrophages (TAMs), limits iNKT cell efficacy. Patient-derived organoid (PDO) platforms provide an innovative model for dissecting these complex interactions evaluating strategies to reinvigorate functionality within TME. PDOs closely mimic genetic, phenotypic, structural characteristics primary tumors, enabling study tumor–immune dynamics. Integrating into offers robust platform investigating CD1d-mediated interactions, Th1-biased responses driven glycolipid analogs like α-GalCer, combination therapies checkpoint inhibitors. Additionally, PDO systems can assess effects metabolic modulation, including reducing lactic acid accumulation or targeting glutamine pathways, on enhancing activity. Emerging innovations, organoid-on-a-chip systems, CRISPR-Cas9 gene editing, multi-omics approaches, further expand potential PDO–iNKT personalized immunotherapy research. Although application in is still undeveloped, hold immense promise bridging preclinical studies clinical translation. By addressing challenges TME optimizing strategies, offer transformative avenue advancing cancer medicine.

Language: Английский

Citations

0
Bronchoalveolar lavage fluid (BALF): Clinical applications for present and future DOI
Jie Mei, Yinong Chen, Huixiang Tian

et al.

The Innovation Medicine, Journal Year: 2025, Volume and Issue: unknown, P. 100115 - 100115

Published: Jan. 1, 2025

<p>Lungs are exposed to a wide range of complex internal and external environmental factors, creating pulmonary microenvironment that remains challenging detect interpret. Bronchoalveolar lavage fluid (BALF) contains an abundance cells, microorganisms, active substances, thus is considered be clinically promising body detection substance representative the microenvironment. The combination experimental strategies with emerging omics technologies has advanced identification interpretation microscopic components in BALF, underscoring its applications clinical detection. In summary, this review provides systematic overview development understanding discusses possible diagnosis, prediction, intervention, highlights role deciphering BALF.</p>

Language: Английский

Citations

0
Evolving from organoid to assembloid with enhanced cellular interactions DOI
Jiarui Liu, Yitong Shi, Xinkun Shen

et al.

Cell organoid (Print), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
TIL Therapy in Lung Cancer: Current Progress and Perspectives DOI Creative Commons

Weilei Hu,

Y. Bian,

Hongbin Ji

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 18, 2024

Lung cancer remains the most prevalent malignant tumor worldwide and is leading cause of cancer-related mortality. Although immune checkpoint blockade has revolutionized treatment advanced lung cancer, many patients still do not respond well, often due to lack functional T cell infiltration. Adoptive therapy (ACT) using expanded cells emerged as an important therapeutic modality. Tumor-infiltrating lymphocytes (TIL) one form ACT involving administration activated autologous derived from surgically resected tissues reinfusion into holds great potential for cancer. In this review, TIL introduced its suitability discussed. Then historical clinical developments are summarized, methods developed up-to-date identify tumor-recognizing TILs optimize composition. Some perspectives toward future also provided.

Language: Английский

Citations

1
Breaking the mold: 3D cell cultures reshaping the future of cancer research DOI Creative Commons
Sandra Cordeiro, Beatriz B P P Oliveira, Rúben Valente

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Nov. 26, 2024

Despite extensive efforts to unravel tumor behavior and develop anticancer therapies, most treatments fail when advanced clinical trials. The main challenge in cancer research has been the absence of predictive models, accurately mimicking tumoral processes response treatments. microenvironment (TME) shows several human-specific physical chemical properties, which cannot be fully recapitulated by conventional 2D cell cultures or

Language: Английский

Citations

1