Low‐intensity pulsed ultrasound improves myocardial ischaemia‒reperfusion injury via migrasome‐mediated mitocytosis

Clinical and Translational Medicine, Journal Year: 2024, Volume and Issue: 14(7)

Published: July 1, 2024

Abstract During myocardial ischaemia‒reperfusion injury (MIRI), the accumulation of damaged mitochondria could pose serious threats to heart. The migrasomes, newly discovered mitocytosis‐mediating organelles, selectively remove provide mitochondrial quality control. Here, we utilised low‐intensity pulsed ultrasound (LIPUS) on MIRI mice model and demonstrated that LIPUS reduced infarcted area improved cardiac dysfunction. Additionally, found alleviated MIRI‐induced We provided new evidence mechanical stimulation facilitated excretion via migrasome‐dependent mitocytosis. Inhibition formation migrasomes abolished protective effect MIRI. Mechanistically, induced by evoking RhoA/Myosin II/F‐actin pathway. Meanwhile, F‐actin activated YAP nuclear translocation transcriptionally activate motor protein KIF5B Drp1, which are indispensable for LIPUS‐induced These results revealed activates mitocytosis, a control mechanism, protect against MIRI, underlining as safe potentially non‐invasive treatment

Language: Английский

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Cardiac fibroblast-derived mitochondria-enriched sEVs regulate tissue inflammation and ventricular remodeling post-myocardial infarction through NLRP3 pathway

Pharmacological Research, Journal Year: 2025, Volume and Issue: unknown, P. 107676 - 107676

Published: Feb. 1, 2025

Resident cardiac fibroblasts (CFs) play crucial roles in sensing injury signals and regulating inflammatory responses post-myocardial infarction (MI). Damaged mitochondria can be transferred extracellularly via various mechanisms, including extracellular vesicles (EVs). In this study, we aimed to investigate whether CFs could transfer damaged mitochondrial components small EVs (sEVs) elucidate their role post-MI. Left anterior descending coronary artery ligation was performed mice. Mitochondrial sEVs were detected using nanoflow cytometry. Differential protein expression from normoxia normoglycemia (CFs-Nor-sEVs) post oxygen-glucose deprivation (CFs-OGD-sEVs) identified label-free proteomics. CFs-sEVs co-cultured with mouse bone marrow-derived macrophages (BMDMs) assess macrophage responses. Effects of intramyocardial injection assessed MI mice the absence or presence NLRP3 inhibitor CY-09. Results demonstrated that CFs-derived enriched CFs-OGD-sEVs (CFs-mt-sEVs), which promoted pro-inflammatory phenotype activation BMDMs vitro. Myocardial CFs-mt-sEVs enhanced tissue inflammation, aggravated dysfunction, exacerbated maladaptive ventricular remodeling post-MI vivo. Mechanistically, above effects achieved reversed by This study indicates post-MI, promote exacerbate activating NLRP3. Our findings highlight potential therapeutic inhibiting improve function attenuate

Language: Английский

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Zinc oxide nanoparticles promote migrasomes formation

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 490, P. 137792 - 137792

Published: Feb. 28, 2025

Language: Английский

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Metformin-Loaded Tannic Acid Nanoparticles Attenuate Osteoarthritis by Promoting Chondrocyte Mitochondria Homeostasis Based on Mitocytosis

Biomacromolecules, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 5, 2025

The oxidative stress microenvironment and mitochondrial dysfunction in chondrocytes are key mechanisms the development of osteoarthritis (OA). Metformin (Met) has demonstrated multiple effects on mitochondria is regarded as a potential therapeutic agent for OA. low blood flow characteristics joint cavity make targeted local delivery metformin crucial its clinical application. In this study, tannic acid (TA), with natural antioxidant anti-inflammatory properties, was used to prepare self-assemble Met-loaded TA nanoparticles (NPs). NPs exhibit excellent reactive oxygen scavenging capability, stability various media, an acid-responsive release Met. Vitro experiments showed that possess biocompatibility, effectively protecting chondrocyte viability OA's pathological environment preventing senescence phenotype. addition, promoted expression elements chondrocytes, restored membrane potential, enhanced mitocytosis improve quality. vivo further confirmed intra-articular injection rats post-traumatic OA improves cartilage matrix degradation, osteophyte formation, subchondral bone sclerosis over 8 weeks. Tissue staining protective mitochondria. Importantly, both vitro revealed provided superior cellular protection compared or Met alone. Overall, study demonstrates against degeneration, advantages easy preparation, high efficiency, biosafety.

Language: Английский

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Migrasomes: Critical players in intercellular nanovesicle communication

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111796 - 111796

Published: April 1, 2025

Language: Английский

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Exploring the role of migrasomes in myocardial injury and repair: A novel mechanism of mitochondrial quality control

Genes & Diseases, Journal Year: 2025, Volume and Issue: unknown, P. 101639 - 101639

Published: April 1, 2025

Language: Английский

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Promoting mitocytosis via gene-engineered aligned fibers for fascia regeneration

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: unknown, P. 113725 - 113725

Published: April 1, 2025

Language: Английский

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Migrasomes as intercellular messengers: potential in the pathological mechanism, diagnosis and treatment of clinical diseases

Journal of Nanobiotechnology, Journal Year: 2025, Volume and Issue: 23(1)

Published: April 20, 2025

Migrasomes are newly identified organelles that were first discovered in 2015. Since then, their biological structure, formation process, and physiological functions have been gradually elucidated. Research recent years has expanded our understanding of these aspects, highlighting significance various pathological processes. found to play crucial roles normal functions, including embryonic development, vascular homeostasis, material transport, mitochondrial quality control. Additionally, emerging evidence suggests involvement diseases; however, clinical research on remains limited. Current studies indicate migrasomes may contribute disease pathogenesis hold potential for diagnostic therapeutic applications. This review consolidates existing migrasomes, focusing role mechanisms use medical By examining structure function, this aims generate insights encourage further research, ultimately contributing advancements prevention treatment.

Language: Английский

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Migrasomes, critical players in intercellular communication

Cancer Cell International, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 25, 2025

Migrasomes are a newly discovered type of extracellular vesicle (EV) formed during cell migration, playing pivotal role in intercellular communication. These vesicles generated by retracting fibers migrating cells and encapsulate various molecules, such as proteins, lipids, RNA, allowing the transfer biochemical signals to neighboring cells. Current evidence suggests that migrasomes involved wide range physiological processes embryogenesis, angiogenesis, immune modulation, mitochondrial quality control. Moreover, implicated pathological conditions, including cancer metastasis, cardiovascular diseases, viral infections. To fully understand their significance, it is critical first explore molecular mechanisms underlying formation function. Recent studies have shed light on biogenesis, release, biological properties migrasomes, all which key understanding cell-to-cell In this review, we provide an up-to-date summary migrasome characterization, activities communication, while also proposing potential new functions for these vesicles.

Language: Английский

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Migrasomes: Emerging organelles for unveiling physiopathology and advancing clinical implications

Life Sciences, Journal Year: 2024, Volume and Issue: unknown, P. 123152 - 123152

Published: Oct. 1, 2024

Language: Английский

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