Role of N6-methyladenosine methylation in nasopharyngeal carcinoma: current insights and future prospective DOI Creative Commons

YaYan Zhao,

Jie Li, Mei-Juan Dian

et al.

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Dec. 18, 2024

Abstract Nasopharyngeal carcinoma (NPC) is a distinct type of head and neck squamous cell prevalent in Southern China, Southeast Asia, North Africa. Despite advances treatment options, the prognosis for advanced NPC remains poor, underscoring urgent need to explore its underlying mechanisms develop novel therapeutic strategies. Epigenetic alterations have been shown play key role progression. Recent studies indicate that dysregulation RNA modifications specifically affects tumor-related transcripts, influencing various oncogenic processes. This review provides comprehensive overview altered their regulators NPC, with focus on m 6 A regulatory mechanisms. We discuss how modification influences gene expression initiation progression at molecular level, analyzing impact cancer-related biological functions. Understanding these could reveal new biomarkers targets offering promising directions future research precision medicine.

Language: Английский

Circular RNA circCLASP2 promotes nasopharyngeal carcinoma progression through binding to DHX9 to enhance PCMT1 translation DOI Creative Commons
Peng Miao, Shan Zhang, Pan Wu

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: March 6, 2025

Circular RNAs (circRNAs), characterized by their covalently closed-loop structures, constitute a distinct class of non-coding RNAs. They play pivotal regulatory roles within cells and are intricately associated with the progression malignant tumors. However, underlying mechanisms in nasopharyngeal carcinoma (NPC) have yet to be fully uncovered comprehensively understood. Employing RNA sequencing technology, high-abundance circular NPC were identified. Expression analysis circCLASP2 tissues was conducted using quantitative real-time polymerase chain reaction (qRT-PCR) situ hybridization experiments. Through vitro vivo functional assays, influence on proliferation metastasis investigated. LC-MS/MS technology analyzed binding partners circCLASP2, its differentially regulated targets, proteins PCMT1. Interactions among DHX9 protein, PCMT1 mRNA elucidated through immunoprecipitation pull-down techniques. The effects G-quadruplex (rG4) structures translation explored immunofluorescence (IF), ribosomal gradient separation, dual-luciferase reporter assays. Immunoprecipitation (IP) revealed downstream effector circCLASP2-DHX9-PCMT1 axis Phalloidin staining confirmed ultimate effect cytoskeleton. PDS treatment applied for interventions NPC, demonstrating potential therapeutic avenues. Our research that novel circRNA has not been reported tumors, is upregulated fosters cell both vivo. Mechanistically, acts as molecular scaffold, facilitating approximation mRNA. unwinds inhibitory rG4 structure near initiation site mRNA, increasing expression. binds upregulates cytoskeleton-associated proteins, modulating cytoskeleton strength dynamics ultimately driving metastasis. In experiments, significantly inhibits growth metastasis, showcasing promising potential. investigation pinpointed RNA, which augments cytoskeletal functions via DHX9-PCMT1 axis, contributing malignancy NPC. This pathway holds promise target Furthermore, these molecules could also serve biomarkers adjunct diagnosis prognosis assessment

Language: Английский

Citations

1
METTL14 promotes lipid metabolism reprogramming and sustains nasopharyngeal carcinoma progression via enhancing m6A modification of ANKRD22 mRNA DOI Creative Commons

Lvyuan Li,

Qiling Tang,

Junshang Ge

et al.

Clinical and Translational Medicine, Journal Year: 2024, Volume and Issue: 14(7)

Published: July 1, 2024

Abstract Background N 6 ‐methyladenosine (m A) modification is essential for modulating RNA processing as well expression, particularly in the context of malignant tumour progression. However, exploration m A nasopharyngeal carcinoma (NPC) remains very limited. Methods levels were analysed NPC using dot blot assay. The expression level methyltransferase‐like 14 (METTL14) within tissues was from public databases RT‐qPCR and immunohistochemistry. influences on METTL14 proliferation metastasis explored via vitro vivo functional assays. Targeted genes screened gene profiling microarray data. Actinomycin D treatment polysome analysis used to detect half‐life translational efficiency ANKRD22. Flow cytometry, immunofluorescence immunoprecipitation validate role ANKRD22 lipid metabolism cells. ChIP‐qPCR H3K27AC signalling near promoters METTL14, GINS3, POLE2, PLEK2 FERMT1 genes. Results We revealed NPC, correlating with poor patient prognosis. In assays indicated actively promoted cells metastasis. catalysed messenger ribonucleic acid (mRNA), recognized by reader IGF2BP2, leading increased mRNA stability higher efficiency. Moreover, ANKRD22, a metabolism‐related protein mitochondria, interacted SLC25A1 enhance citrate transport, elevating intracellular acetyl‐CoA content. This dual impact reprogramming cellular synthesis while upregulating associated cell cycle (GINS3 POLE2) cytoskeleton (PLEK2 FERMT1) through heightened epigenetic histone acetylation nucleus. Intriguingly, our findings highlighted elevated ANKRD22‐mediated H3 lysine 27 (H3K27AC) signals promoter, which contributes positive feedback loop perpetuating progression NPC. Conclusions identified METTL14‐ANKRD22‐SLC25A1 axis emerges promising therapeutic target also these molecules may serve novel diagnostic biomarkers.

Language: Английский

Citations

7
Seven Genes Involved in Cancer Metastasis DOI Creative Commons
Mina Khajehdehi

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

Metastasis means detachment, survival and dissemination via the circulatory system, colonization in a distant organ. It is complex phenomenon, there not much information about its starting progression causes. Recognition of molecular/cellular basis opens new insights into control. This lethal process cancer mostly associated with epithelial-mesenchymal transition (EMT) activation. Based on research, some abnormalities gene expression facilitate acquiring metastatic traits. Here, we present abnormality effect seven genes invasion migration. These have recently been great interest to researchers investigate their relationship aggressive behavior cancer. Up-regulation or down-regulation them may promote inhibit different cancers, dual various types They influence EMT-related by regulating MAPK PI3K/AKT signaling pathways. The WNT/β-catenin STAT3 pathways are subsequent ranks.

Language: Английский

Citations

0
Covalent binding of 5-tetradecyloxy-2-furoic acid (TOFA) and c(RGDfK) and its co-delivery with Lipusu, a novel synergistic strategy to inhibit the proliferation of nasopharyngeal cancer DOI Creative Commons
Min Feng,

Wei Gong,

Xin Zhu

et al.

European Journal of Pharmaceutical Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 107092 - 107092

Published: April 1, 2025

Language: Английский

Citations

0
Improved VPS4B O-GlcNAc modification triggers lipid droplets transferring from adipocytes to nasopharyngeal carcinoma cells DOI Creative Commons
Haimeng Yin,

Ying Chun Shan,

Qin Zhu

et al.

Cancer & Metabolism, Journal Year: 2025, Volume and Issue: 13(1)

Published: May 23, 2025

The tumor microenvironment (TME) supplies critical metabolites that support cancer cell survival and progression. Adipocytes progression by secreting free fatty acids (FFAs) adipokines; however, the role mechanisms underlying lipid droplet (LD) release from adipocytes remain elusive. Using two nasopharyngeal carcinoma (NPC) lines primary human pre-adipocytes (HPA), we evaluate effect of LDs on growth, proliferation, colony formation, migration. We also assess roles LD in vivo. RNA-seq analysis, elucidate hypoxic NPC cell-derived exosomes (H-exo) gene expression profile adipocytes. By co-culture system, investigated vacuolar protein sorting 4 homolog B (VPS4B)-annexin A5 (ANXA5) interaction adipocyte maturity release. Herein, report LDs, rather than FFAs, are form transferred to cells, enhancing cells internalize directly via macropinocytosis, while H-exo induces oxidative stress membrane fluidity adipocytes, leading Transcriptomic proteomic analyses reveal VPS4B triggers interacting with ANXA5, low LKB1 enhances O-linked N-acetylglucosamine (O-GlcNAc) modification through inhibition serine/threonine kinase 11 (STK11/LKB1)-AMP-activated (AMPK) pathway activation hexosamine biosynthesis (HBP) flux. This study uncovers transfer TME, suggesting new therapeutic avenues NPC.

Language: Английский

Citations

0
The emerging significance of the METTL family as m6A-modified RNA methyltransferases in head and neck cancer DOI
Ming Yang,

Zile Zhang,

Hao Qin

et al.

Cellular Signalling, Journal Year: 2025, Volume and Issue: unknown, P. 111798 - 111798

Published: April 1, 2025

Language: Английский

Citations

0
Role of N6-methyladenosine methylation in nasopharyngeal carcinoma: current insights and future prospective DOI Creative Commons

YaYan Zhao,

Jie Li, Mei-Juan Dian

et al.

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: Dec. 18, 2024

Abstract Nasopharyngeal carcinoma (NPC) is a distinct type of head and neck squamous cell prevalent in Southern China, Southeast Asia, North Africa. Despite advances treatment options, the prognosis for advanced NPC remains poor, underscoring urgent need to explore its underlying mechanisms develop novel therapeutic strategies. Epigenetic alterations have been shown play key role progression. Recent studies indicate that dysregulation RNA modifications specifically affects tumor-related transcripts, influencing various oncogenic processes. This review provides comprehensive overview altered their regulators NPC, with focus on m 6 A regulatory mechanisms. We discuss how modification influences gene expression initiation progression at molecular level, analyzing impact cancer-related biological functions. Understanding these could reveal new biomarkers targets offering promising directions future research precision medicine.

Language: Английский

Citations

1