Biomarker Research,
Journal Year:
2023,
Volume and Issue:
11(1)
Published: April 17, 2023
Abstract
N6-methyladenosine
(m
6
A)
is
considered
as
the
most
common
and
important
internal
transcript
modification
in
several
diseases
like
type
2
diabetes,
schizophrenia
especially
cancer.
As
a
main
target
of
m
A
methylation,
long
non-coding
RNAs
(lncRNAs)
have
been
proved
to
regulate
cellular
processes
at
various
levels,
including
epigenetic
modification,
transcriptional,
post-transcriptional,
translational
post-translational
regulation.
Recently,
accumulating
evidence
suggests
that
A-modified
lncRNAs
greatly
participate
tumorigenesis
cancers.
In
this
review,
we
systematically
summarized
biogenesis
identified
A-lncRNAs
variety
cancers,
well
their
potential
diagnostic
therapeutic
applications
biomarkers
targets,
hoping
shed
light
on
novel
strategies
for
cancer
treatment.
Experimental Hematology and Oncology,
Journal Year:
2022,
Volume and Issue:
11(1)
Published: Aug. 9, 2022
Abstract
The
N(6)-methyladenosine
(m6A)
modification
is
the
most
pervasive
of
human
RNAs.
In
recent
years,
an
increasing
number
studies
have
suggested
that
m6A
likely
plays
important
roles
in
cancers.
Many
demonstrated
involved
biological
functions
cancer
cells,
such
as
proliferation,
invasion,
metastasis,
and
drug
resistance.
addition,
closely
related
to
prognosis
patients.
this
review,
we
highlight
advances
understanding
function
various
We
emphasize
importance
progression
look
forward
describe
future
research
directions.
Clinical and Translational Medicine,
Journal Year:
2021,
Volume and Issue:
11(6)
Published: June 1, 2021
Abstract
Background
Bone
metastasis
is
the
leading
cause
of
tumor‐related
death
in
prostate
cancer
(PCa)
patients.
Long
noncoding
RNAs
(lncRNAs)
have
been
well
documented
to
be
involved
progression
multiple
cancers.
Nevertheless,
role
lncRNAs
PCa
bone
remains
largely
unclear.
Methods
The
expression
cancer‐associated
transcripts
was
analyzed
published
datasets
and
further
verified
clinical
samples
cell
lines
by
RT‐qPCR
situ
hybridization
assays.
Colony
formation
assay,
MTT
cycle
analysis,
EdU
Transwell
migration
invasion
assays,
wound
healing
vivo
experiments
were
carried
out
investigate
function
transcript
6
(
PCAT6
)
tumor
growth
PCa.
Bioinformatic
RNA
pull‐down,
RIP
assays
conducted
identify
proteins
binding
potential
targets
.
therapeutic
targeting
antisense
oligonucleotides
(ASO)
explored
Results
upregulated
tissues
with
increased
predicted
poor
prognosis
Functional
found
that
knockdown
significantly
inhibited
invasion,
migration,
proliferation
vitro
,
as
Mechanistically,
METTL3
‐mediated
m
A
modification
contributed
upregulation
an
IGF2BP2
‐dependent
manner.
Furthermore,
IGF1R
enhancing
mRNA
stability
through
/
RNA‐protein
three‐dimensional
complex.
Importantly,
inhibition
ASO
showed
against
Finally,
correlation
demonstrated
cells.
Conclusions
Our
study
uncovers
a
novel
molecular
mechanism
which
A‐induced
axis
promotes
growth,
suggesting
may
serve
promising
prognostic
marker
target
bone‐metastatic
Biomarker Research,
Journal Year:
2023,
Volume and Issue:
11(1)
Published: June 6, 2023
N6-methyladenosine
(m6A)
is
the
most
prevalent
and
well-characterized
internal
chemical
modification
in
eukaryotic
RNA,
influencing
gene
expression
phenotypic
changes
by
controlling
RNA
fate.
Insulin-like
growth
factor-2
mRNA-binding
proteins
(IGF2BPs)
preferentially
function
as
m6A
effector
proteins,
promoting
stability
translation
of
m6A-modified
RNAs.
IGF2BPs,
particularly
IGF2BP1
IGF2BP3,
are
widely
recognized
oncofetal
predominantly
expressed
cancer
rather
than
normal
tissues,
playing
a
critical
role
tumor
initiation
progression.
Consequently,
IGF2BPs
hold
potential
for
clinical
applications
serve
good
choice
targeted
treatment
strategies.
In
this
review,
we
discuss
functions
mechanisms
readers
explore
therapeutic
targeting
human
cancer.
International Journal of Biological Sciences,
Journal Year:
2022,
Volume and Issue:
18(7), P. 2744 - 2758
Published: Jan. 1, 2022
RNA
can
be
modified
by
over
170
types
of
distinct
chemical
modifications,
and
the
most
abundant
internal
modification
mRNA
in
eukaryotes
is
N6-methyladenosine
(m
Genes & Diseases,
Journal Year:
2023,
Volume and Issue:
11(2), P. 890 - 920
Published: July 20, 2023
m6A
methylation
is
the
most
frequent
modification
of
mRNA
in
eukaryotes
and
plays
a
crucial
role
cancer
progression
by
regulating
biological
functions.
Insulin-like
growth
factor
2
mRNA-binding
proteins
(IGF2BP)
are
newly
identified
'readers'.
They
belong
to
family
RNA-binding
proteins,
which
bind
sites
on
different
RNA
sequences
stabilize
them
promote
progression.
In
this
review,
we
summarize
mechanisms
upstream
factors
regulate
IGF2BP
cancer.
The
current
literature
analyzed
here
reveals
that
cell
proliferation,
survival,
chemoresistance,
inhibit
apoptosis,
also
associated
with
glycolysis,
angiogenesis,
immune
response
tumor
microenvironment.
Therefore,
discovery
their
as
'readers'
characteristic
re-expression
IGF2BPs
cancers,
it
important
elucidate
mechanism
action
immunosuppressive
We
describe
detail
regulatory
interaction
network
downstream
target
RNAs
discuss
potential
clinical
applications
diagnostic
prognostic
markers,
well
recent
advances
biology
therapeutic
value.
Pharmacological Research,
Journal Year:
2023,
Volume and Issue:
194, P. 106775 - 106775
Published: April 17, 2023
Prostate
carcinoma
is
a
malignant
situation
that
arises
from
genomic
alterations
in
the
prostate,
leading
to
changes
tumorigenesis.
The
NF-κB
pathway
modulates
various
biological
mechanisms,
including
inflammation
and
immune
responses.
Dysregulation
of
promotes
carcinogenesis,
increased
proliferation,
invasion,
therapy
resistance.
As
an
incurable
disease
globally,
prostate
cancer
significant
health
concern,
research
into
genetic
mutations
function
has
efficacy
facilitate
introduction
novel
therapies.
upregulation
observed
during
progression,
resulting
cell
cycle
progression
proliferation
rates.
Additionally,
endorses
resistance
death
enhances
capacity
for
metastasis,
particularly
bone
metastasis.
Overexpression
triggers
chemoresistance
radio-resistance,
inhibition
by
anti-tumor
compounds
can
reduce
progression.
Interestingly,
non-coding
RNA
transcripts
regulate
level
its
nuclear
transfer,
offering
potential
avenue
modulating
Cancer Gene Therapy,
Journal Year:
2024,
Volume and Issue:
31(6), P. 816 - 830
Published: Feb. 14, 2024
Abstract
RNA
modification,
especially
N6-methyladenosine,
5-methylcytosine,
and
N7-methylguanosine
methylation,
participates
in
the
occurrence
progression
of
cancer
through
multiple
pathways.
The
function
expression
these
epigenetic
regulators
have
gradually
become
a
hot
topic
research.
Mutation
regulation
noncoding
RNA,
lncRNA,
play
major
role
cancer.
Generally,
lncRNAs
exert
tumor-suppressive
or
oncogenic
functions
its
dysregulation
can
promote
tumor
metastasis.
In
this
review,
we
summarize
modifications
lncRNAs.
Furthermore,
discuss
relationship
between
modification
lncRNA
interaction
various
cancers.
Therefore,
review
gives
comprehensive
understanding
mechanisms
by
which
affects
cancers
regulating
lncRNAs,
may
shed
new
light
on
research
provide
insights
into
therapy.
Journal of Experimental & Clinical Cancer Research,
Journal Year:
2024,
Volume and Issue:
43(1)
Published: Jan. 29, 2024
Abstract
Background
The
development
of
radioresistance
seriously
hinders
the
efficacy
radiotherapy
in
lung
cancer.
However,
underlying
mechanisms
by
which
occurs
are
still
incompletely
understood.
N
6
-Methyladenosine
(m
A)
modification
RNA
is
involved
cancer
progression,
but
its
role
remains
elusive.
This
study
aimed
to
identify
m
A
regulators
radiosensitivity
and
further
explore
therapeutic
targets
overcome
radioresistance.
Methods
Bioinformatic
mining
was
used
regulator
IGF2BP2
radiosensitivity.
Transcriptome
sequencing
downstream
factors.
Clonogenic
survival
assays,
neutral
comet
Rad51
foci
formation
Annexin
V/propidium
iodide
assays
were
determine
significance
FBW7/IGF2BP2/SLC7A5
axis
Chromatin
immunoprecipitation
(ChIP)-qPCR
analyses,
(RIP)
methylated
(MeRIP)-qPCR
pull-down
co-immunoprecipitation
ubiquitination
feedback
loop
between
SLC7A5
regulatory
effect
FBW7/GSK3β
on
IGF2BP2.
Mice
models
tissue
microarrays
verify
effects
vivo.
Results
We
identified
IGF2BP2,
an
“reader”,
that
overexpressed
facilitates
showed
inhibition
impairs
both
vitro
Furthermore,
we
found
enhances
stability
translation
mRNA
through
modification,
resulting
enhanced
SLC7A5-mediated
transport
methionine
produce
S-adenosylmethionine.
feeds
back
upon
promoter
region
increasing
trimethyl
at
lysine
4
histone
H3
(H3K4me3)
level
upregulate
expression.
demonstrated
this
positive
promotes
AKT/mTOR
pathway.
Moreover,
ubiquitin
ligase
FBW7
functions
with
GSK3β
kinase
recognize
degrade
Conclusions
Collectively,
our
revealed
“reader”
forming
a
SLC7A5,
suggesting
may
be
potential
target
control
International Journal of Biological Sciences,
Journal Year:
2022,
Volume and Issue:
18(16), P. 6145 - 6162
Published: Jan. 1, 2022
Background:
N6-methyladenosine
(m6A)
is
one
of
the
most
prevalent
mRNA
modifications
in
mammals,
and
it
regulates
fate
modified
RNA
transcripts.In
current
study,
we
aimed
to
elucidate
role
YTH
m6A
RNA-binding
protein
1
(YTHDF1),
a
"reader"
modification,
prostate
cancer
tumorigenesis.Methods:
We
employed
multi-omics
approach
detect
direct
target
YTHDF1
upon
manipulation
expression
cells.Expression
was
also
evaluated
human
tumors
either
adjacent
or
paired
normal
tissues.Additionally,
vivo
tumor
growth
metastasis
experimental
assays
were
performed
evaluate
tumorigenesis.Finally,
luciferase
reporter
Chromatin
immunoprecipitation
(ChIP)
conducted
transcriptional
regulators
YTHDF1.Results:
demonstrated
that
polo-like
kinase
(PLK1)
YTHDF1.YTHDF1
facilitated
translation
efficiency
PLK1
an
m6A-dependent
manner
by
identifying
m6A-modified
subsequently
promoted
hyperactivation
PI3K/AKT
signaling
pathway.Moreover,
our
results
indicated
upregulated
tissue
high
associated
with
adverse
prognosis
patients
cancer.Furthermore,
upregulation
tumorigenesis
vitro
vivo.Additionally,
dysregulation
ETS
transcription
factor
ELK1
activated
directly
binding
its
promoter
region.Conclusions:
Collectively,
findings
suggest
ELK1/YTHDF1/PLK1/PI3K/AKT
axis
critical
for
progression
may
serve
as
potential
therapeutic
treatment.