
Clinical and Translational Medicine, Journal Year: 2025, Volume and Issue: 15(3)
Published: Feb. 25, 2025
Immunotherapy shows promise for treating advanced breast cancer, but only a few patients could respond. Predictive biomarkers from peripheral blood are urgently needed. We designed comprehensive 42-marker mass cytometry panel to profile the samples 57 diagnosed with HER2-negative cancer receiving anti-PD-1 combination therapy. Patients were categorized as responders and non-responders according 6-month progression-free survival (PFS), followed by phenotypic functional comparations identify candidate predictive biomarkers. Longitudinal analysis of paired further revealed dynamic changes in these specific subpopulations. Non-responders exhibited significantly higher frequencies CD39+ Tregs (adjusted p = .031) T-cell milieu at baseline, which positive correlation PD-1+ T cells NR group. assessment indicated significant decrease an increase following treatment, suggesting their potential role immunotherapy resistance. In myeloid compartment, showed CCR2+ monocyte-derived dendritic cell than .037). These positively correlated other negatively naïve non-responders. Based on two efficacy-related biomarkers, we developed prognostic prediction model confirmed its superiority distinguishing patient PFS (p < .001). Peripheral response, serving guide therapeutic choices immunotherapy. associated poor response cancer. Higher correlate better outcomes. A based effectively distinguishes survival. offer non-invasive approach choices.
Language: Английский