Chip-Based Optoacoustic Single-Cell Detection in Flow Using Point-Source Optimized Surface Acoustic Wave Transducers DOI Creative Commons
Simon Göllner, Melanie Colditz,

Yishu Huang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Sensitive measurement of the optoacoustic (OA, also photoacoustic) properties cells in flow is highly desirable, as it provides information about optical absorption cellular compounds. Hence, spectral characteristics can deliver cell state or disease parameters, but be used for high-throughput sorting by intrinsic without additional fluorescence labeling. The current implementation measurements a microfluidic context typically relies on piezoelectric (ultrasound) transducers attached to chip, whereby transducer records ultrasound signal originating from absorbing species when excited laser pulses. arrangement outside chip leads challenge integration over larger area and coupling interlayer effects resulting attenuation reduction sensitivity. Moreover, placement bulky prevents exploitation full advantages microfluidics. As solution, we demonstrate use point-source optimized interdigital (pIDTs) directly fabricated surface detection acoustic waves (SAW) single continuous flow. SAW bulk effect inside illuminated light. these focused pIDTs on-chip lithographically hard-wall microchannels allows with spatial resolution order diameter on-chip, offering possibility miniaturization, parallelization, cheap mass production.

Language: Английский

Photoacoustic imaging for cutaneous melanoma assessment: a comprehensive review DOI Creative Commons

Joseph W. Fakhoury,

Juliana Benavides, Rayyan Manwar

et al.

Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(S1)

Published: Jan. 12, 2024

SignificanceCutaneous melanoma (CM) has a high morbidity and mortality rate, but it can be cured if the primary lesion is detected treated at an early stage. Imaging techniques such as photoacoustic (PA) imaging (PAI) have been studied implemented to aid in detection diagnosis of CM.AimProvide overview different PAI systems applications for study CM, including determination tumor depth/thickness, cancer-related angiogenesis, metastases lymph nodes, circulating cells (CTCs), virtual histology, studies using exogenous contrast agents.ApproachA systematic review classification configurations was conducted based on their specific detection. This encompasses animal preclinical studies, offering insights into future potential clinic.ResultsPAI holds great clinical noninvasive technique disease management. PA microscopy predominantly used image angiogenesis surrounding tumors provide information characteristics. Additionally, tomography, with its increased penetration depth, demonstrated ability assess thickness. Both modalities shown promise detecting nodes CTCs, all-optical implementation developed perform histology analyses. Animal human successfully capability detect, visualize, classify, stage CM.ConclusionsPAI promising assessing status skin without surgical procedure. The modality microvasculature, visualize boundaries, detect fast label-free identify CTCs could metastatic status. In addition, useful monitoring treatment efficacy noninvasively.

Language: Английский

Citations

12
Chip-Based Optoacoustic Single-Cell Detection in Flow Using Point-Source Optimized Surface Acoustic Wave Transducers DOI Creative Commons
Simon Göllner, Melanie Colditz,

Yishu Huang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Sensitive measurement of the optoacoustic (OA, also photoacoustic) properties cells in flow is highly desirable, as it provides information about optical absorption cellular compounds. Hence, spectral characteristics can deliver cell state or disease parameters, but be used for high-throughput sorting by intrinsic without additional fluorescence labeling. The current implementation measurements a microfluidic context typically relies on piezoelectric (ultrasound) transducers attached to chip, whereby transducer records ultrasound signal originating from absorbing species when excited laser pulses. arrangement outside chip leads challenge integration over larger area and coupling interlayer effects resulting attenuation reduction sensitivity. Moreover, placement bulky prevents exploitation full advantages microfluidics. As solution, we demonstrate use point-source optimized interdigital (pIDTs) directly fabricated surface detection acoustic waves (SAW) single continuous flow. SAW bulk effect inside illuminated light. these focused pIDTs on-chip lithographically hard-wall microchannels allows with spatial resolution order diameter on-chip, offering possibility miniaturization, parallelization, cheap mass production.

Language: Английский

Citations

0