Identification and genetic characterization of Xiabuqu River virus: A novel member of the Iflaviridae family detected in soft ticks from Tibet, China

Virus Research, Journal Year: 2025, Volume and Issue: 354, P. 199543 - 199543

Published: Feb. 20, 2025

Soft ticks, an important group of blood-sucking arthropods in nature, are widely distributed globally and can carry a wide range pathogens, including Theileria ovis, Anaplasma Rickettsia spp. African swine fever virus. In this study, we identified novel single positive-stranded RNA virus, tentatively named Xiabuqu River virus (XRV), for the first time from soft ticks collected Shigatse, Tibet. A total 96 engorged were Tibetan sheep, with each tick assigned to separate pool analysis. The complete coding sequence XRV was determined through next-generation sequencing, revealing length 9277 nucleotides that includes open reading frame (ORF) encoding proteins such as capsid protein, helicase, RNA-dependent polymerase (RDRP). Quantitative RT-PCR nested PCR utilized investigate distribution within sample. Pairwise distance analysis revealed all obtained viral sequences shared high nucleotide identity. Phylogenetic demonstrated clustered Lhasa Iflav 1, Fuyun 2, Hubei 2. Further analyses indicated is new member unclassified genus Iflavirus family Iflaviridae.

Language: Английский

Host genetics and larval host plant modulate microbiome structure and evolution underlying the intimate insect–microbe–plant interactions in Parnassius species on the Qinghai‐Tibet Plateau

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(4)

Published: April 1, 2024

Insects harbor a remarkable diversity of gut microbiomes critical for host survival, health, and fitness, but the mechanism this structured symbiotic community remains poorly known, especially insect group consisting many closely related species that inhabit Qinghai-Tibet Plateau. Here, we firstly analyzed population-level 16S rRNA microbial dataset, comprising 11

Language: Английский

Functions and regulations of insect gut bacteria

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(10), P. 4828 - 4840

Published: June 17, 2024

Abstract The insect gut is a complicated ecosystem that inhabits large number of symbiotic bacteria. As an important organ the host insect, bacteria play very roles in regulating physiological and metabolic processes. Recently, much progress has been made study guts with development high‐throughput sequencing technology molecular biology. This review summarizes primary functions guts, such as enhancing insecticide resistance, facilitating food digestion, promoting detoxification, mating behavior egg hatching. It also addresses some possible pathways symbiont regulation governed by external habitats, conditions immunity insect. provides solid foundations for further studies on novel theories, new technologies practical applications guts. © 2024 Society Chemical Industry.

Language: Английский