Microbiome and climate: Skin microbial diversity and community functions ofPolypedates megacephalus(Anura: Rhacophoridae) associated with bioclimate DOI Creative Commons
Dan Sun, Yewei Liu, Shipeng Zhou

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

ABSTRACT The microbiome inhabiting animal skin plays a crucial role in host fitness by influencing both the composition and function of microbial communities. Environmental factors, including climate, significantly impact diversity functional attributes these However, it remains unclear how specific climatic factors affect amphibian composition, community function, relationship between two aspects. Given that amphibians are poikilotherms, thus more susceptible to temperature fluctuations, understanding effects is particularly important. Here, we investigated rhacophorid tree frog Polypedates megacephalus across different regimes using 16S rRNA gene sequencing. Skin swab samples were collected from nine populations P. adults Guangxi region, China. majority core microbiota found belong genus Pseudomonas . Our findings indicate diversity, associated with changes conditions. Specifically, taxonomic increased response greater climate variability, fluctuations. Additionally, communities changed parallel shifts suggesting environmental filtering driven negatively impacts redundancy. These results highlight critical influence on microbiomes offer new insights into contribute adaptation varying IMPORTANCE This study important association amphibians, vulnerable due their poikilothermic nature. Amphibians rely for key functions like disease resistance, yet little known about fluctuations By analyzing regimes, our analysis reveals while variability enhances reduces potential ecological consequences change emphasize need integrate health conservation strategies.

Language: Английский

Recent Progress in Biomedical Scaffold Fabricated via Electrospinning: Design, Fabrication and Tissue Engineering Application DOI
Feng Cheng, Danyang Song, Hongbin Li

et al.

Advanced Functional Materials, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Abstract Electrospinning is a significant manufacturing strategy to create micro/nanofiber platforms that can be considered biomedical scaffold for tissue engineering repair and regeneration. In recent years researchers have continuously broadened the equipment design materials development of electrospinning nanofiber (ENPs), which evolved from single‐needle multi‐needle creating 3D ENPs, diversify their application including drugs/cell/growth factors release, anti‐bacterial anti‐inflammatory, hemostasis, wound healing, Herein, multifunctional ENPs with bioactive polymer fabricated via in terms novel material design, construction various structures, requirements different regeneration are reviewed. Furthermore, this review delves into advancements facilitated by highlighting effectiveness versatility across types such as bone, cartilage, tendons, cardiac tissue, nerves. The discussion comprehensively addresses ongoing challenges selection, biodegradation mechanisms, bioactivation strategies, techniques specific applications. Moreover, outlines potential future research avenues aimed at enhancing ENPs‐based approaches engineering. This in‐depth analysis aims provide nuanced insights technical recommendations propel field forward

Language: Английский

Citations

11
Blurred Lines Between Determinism and Stochasticity in an Amphibian Phylosymbiosis Under Pathogen Infection DOI Open Access
Ana V. Longo, Jaiber J. Solano‐Iguaran, Andrés Valenzuela‐Sánchez

et al.

Molecular Ecology, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

ABSTRACT Selection, dispersal and drift jointly contribute to generating variation in microbial composition within between hosts, habitats ecosystems. However, we have limited examples of how these processes interact as hosts their microbes turn over across latitudinal gradients biodiversity climate. To bridge this gap, assembled an extensive dataset 580 skin bacteriomes from 22 amphibian species distributed a 10° range Chile. Amphibians are susceptible the fungal pathogen Batrachochytrium dendrobatidis ( Bd ), which infects skin, potentially leading changes normal microbiome (i.e., dysbiosis). Using comparative methods, accounting for infection implementing resampling schemes, found evidence phylosymbiosis, characterised by more similar bacterial communities closely related species. We also compared neutral affected assembly bacteria focusing on two widespread our dataset: Chilean four‐eyed frog Pleurodema thaul ) Darwin's Rhinoderma darwinii ). Neutral models revealed that chance largely facilitated occurrence ~90% both Deterministic (e.g., active recruitment microbes, microbe–microbe interactions) explained remaining fraction bacteriomes. Amphibian accounted 21%–32% variance non‐neutral taxa, whereas interaction with carried weaker but still significant effect. Our findings provide ectotherms most subject chance, yet contemporary historical contingencies leave strong signatures microbiomes even at large geographical scales.

Language: Английский

Citations

0
Microbiome and climate: skin microbial diversity and community functions of Polypedates megacephalus (Anura: Rhacophoridae) associated with bioclimate DOI Creative Commons
Dan Sun, Yewei Liu, Shipeng Zhou

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

The microbiome inhabiting animal skin plays a crucial role in host fitness by influencing both the composition and function of microbial communities. Environmental factors, including climate, significantly impact diversity functional attributes these However, it remains unclear how specific climatic factors affect amphibian composition, community function, relationship between two aspects. Understanding effects is particularly important because amphibians are poikilotherms and, thus, more susceptible to temperature fluctuations. Here, we investigated rhacophorid tree frog Polypedates megacephalus across different regimes using 16S rRNA gene sequencing. Skin swab samples were collected from nine populations P. adults Guangxi region, China. majority core microbiota found belong genus Pseudomonas. Our findings indicate that diversity, associated with changes conditions. Specifically, taxonomic increased response higher climate variability, Additionally, traits communities changed parallel shifts composition. significant correlations redundancy index suggest environmental filtering driven change impacts stability. These results highlight critical influence on microbiomes offer new insights into contribute adaptation varying conditions.IMPORTANCEThis study understanding association amphibians, which vulnerable due their poikilothermic nature. Amphibians rely for key functions like disease resistance, yet little known about fluctuations By analyzing regimes, our analysis reveals warmer climates could reduce redundancy, indicating stability be hosts adapted relatively cooler potential ecological consequences emphasize need integrate health conservation strategies.

Language: Английский

Citations

0
Microbiome and climate: Skin microbial diversity and community functions ofPolypedates megacephalus(Anura: Rhacophoridae) associated with bioclimate DOI Creative Commons
Dan Sun, Yewei Liu, Shipeng Zhou

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 16, 2024

ABSTRACT The microbiome inhabiting animal skin plays a crucial role in host fitness by influencing both the composition and function of microbial communities. Environmental factors, including climate, significantly impact diversity functional attributes these However, it remains unclear how specific climatic factors affect amphibian composition, community function, relationship between two aspects. Given that amphibians are poikilotherms, thus more susceptible to temperature fluctuations, understanding effects is particularly important. Here, we investigated rhacophorid tree frog Polypedates megacephalus across different regimes using 16S rRNA gene sequencing. Skin swab samples were collected from nine populations P. adults Guangxi region, China. majority core microbiota found belong genus Pseudomonas . Our findings indicate diversity, associated with changes conditions. Specifically, taxonomic increased response greater climate variability, fluctuations. Additionally, communities changed parallel shifts suggesting environmental filtering driven negatively impacts redundancy. These results highlight critical influence on microbiomes offer new insights into contribute adaptation varying IMPORTANCE This study important association amphibians, vulnerable due their poikilothermic nature. Amphibians rely for key functions like disease resistance, yet little known about fluctuations By analyzing regimes, our analysis reveals while variability enhances reduces potential ecological consequences change emphasize need integrate health conservation strategies.

Language: Английский

Citations

0