Molecular mechanisms of sperm motility are conserved in a basal metazoan DOI Creative Commons
Kelsey F. Speer, Luella Allen‐Waller, Dana Novikov

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: May 29, 2021

Abstract Efficient and targeted sperm motility is essential for animal reproductive success. Studies in mammals echinoderms have uncovered a highly conserved signaling mechanism which stimulated by pH-dependent activation of the cAMP-producing enzyme soluble adenylyl cyclase (sAC). However, presence this pathway basal metazoans has, until now, been unexplored. Here we found that cytoplasmic alkalinization induced rapid burst cAMP full from reef-building coral Montipora capitata . Coral expressed sAC flagellum, midpiece, acrosomal regions, indicating molecular pH sensor may play role regulating mitochondrial respiration flagellar beating. In bilaterians, central node broader alters cellular behavior response to changes extracellular environment. We present transcript-level evidence homologous sperm, including Na + /H exchanger SLC9C1, protein kinase A, CatSper Ca 2+ channel even mammalian sperm. Our discovery metazoan species highlights ancient origin pH-sAC-cAMP physiology suggests it be many other marine invertebrate taxa mechanisms remain These results emphasize our need better understand success, particularly as worsening ocean acidification warming due climate change continue impair corals invertebrates. Statement significance Reef-building are keystone world’s most biodiverse yet threatened ecosystems. Corals reproduce broadcast spawning, making ability their swim through water column fertilization. little known about regulate motility. elevated intracellular promotes production second messenger triggers onset This study reveals deep conservation humans corals, presenting first comprehensive examination an ancestral animal. critical understanding resilience sensitive life stage changing

Language: Английский

Gamete-mediated mate choice: towards a more inclusive view of sexual selection DOI Open Access
Jukka Kekäläinen, Jonathan P. Evans

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2018, Volume and Issue: 285(1883), P. 20180836 - 20180836

Published: July 18, 2018

‘Sperm competition’—where ejaculates from two or more males compete for fertilization—and ‘cryptic female choice’—where females bias this contest to suit their reproductive interests—are now part of the everyday lexicon sexual selection. Yet physiological processes that underlie these post-ejaculatory episodes selection remain largely enigmatic. In review, we focus on a range cellular- and molecular-level processes, known be fundamental fertilization across most (if not all) sexually reproducing species, point putative role in facilitating at level cells gametes, called ‘gamete-mediated mate choice’ (GMMC). way, collate accumulated evidence GMMC different mating systems, emphasize evolutionary significance such non-random interactions among gametes. Our overall aim review is build inclusive view by showing choice often acts nuanced ways than has traditionally been assumed. We also bridge conceptual divide between proximal mechanisms reproduction, adaptive explanations patterns sperm–egg are emerging an increasingly diverse array taxa.

Language: Английский

Citations

85
Acid times in physiology: A systematic review of the effects of ocean acidification on calcifying invertebrates DOI
Isadora Porto Martins Medeiros, Marta Marques Souza

Environmental Research, Journal Year: 2023, Volume and Issue: 231, P. 116019 - 116019

Published: April 28, 2023

Language: Английский

Citations

15
Molecular mechanisms of sperm motility are conserved in an early-branching metazoan DOI Creative Commons
Kelsey F. Speer, Luella Allen‐Waller, Dana Novikov

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2021, Volume and Issue: 118(48)

Published: Nov. 22, 2021

Significance Reef-building corals are the keystone species of world’s most biodiverse yet threatened marine ecosystems. Coral reproduction, critical for reef resilience, requires that coral sperm swim through water column to reach egg. However, little is known about mechanisms regulate motility. We found here motility pH dependent and activation signaling via pH-sensing enzyme soluble adenylyl cyclase. This study reveals deep conservation a pathway from humans corals, presenting first comprehensive examination molecular regulating in an early-diverging animal. These results understanding resilience this sensitive life stage changing environment.

Language: Английский

Citations

27
Ocean acidification does not overlook sex: Review of understudied effects and implications of low pH on marine invertebrate sexual reproduction DOI Creative Commons
Jacqueline L. Padilla‐Gamiño,

Lindsay Alma,

Laura H. Spencer

et al.

Frontiers in Marine Science, Journal Year: 2022, Volume and Issue: 9

Published: Oct. 28, 2022

Sexual reproduction is a fundamental process essential for species persistence, evolution, and diversity. However, unprecedented oceanographic shifts due to climate change can impact physiological processes, with important implications sexual reproduction. Identifying bottlenecks vulnerable stages in reproductive cycles will enable better prediction of the organism, population, community, global-level consequences ocean change. This article reviews how acidification impacts processes marine invertebrates highlights current research gaps. We focus on five economically ecologically taxonomic groups: cnidarians, crustaceans, echinoderms, molluscs ascidians. discuss spatial temporal variability experimental designs, identify trends performance acidified conditions context early traits (gametogenesis, fertilization, resource allocation), provide quantitative meta-analysis published literature assess effects low pH fertilization rates across taxa. A total 129 studies investigated 122 selected The dependent taxa, specific examined, study location. Our reveals that rate decreases as decreases, but are taxa-specific. Echinoderm appears more sensitive than changes, while data limited, cnidarians may be most sensitive. Studies echinoderms bivalve prevalent, crustaceans cephalopods among least studied even though they constitute some largest fisheries worldwide. lack information has commercial aquaculture, wild fisheries, conservation restoration populations. recommend expose organisms different levels during entire gametogenic cycle, not only final before gametes or larvae released. argue increased associated molecular mechanisms chemistry. recommendations future allow understanding affected rapidly changing environment.

Language: Английский

Citations

16
Post-ejaculation thermal stress causes changes to the RNA profile of sperm in an external fertilizer DOI Open Access
Rowan A. Lymbery, Jonathan P. Evans, W. Jason Kennington

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2020, Volume and Issue: 287(1938), P. 20202147 - 20202147

Published: Nov. 11, 2020

Sperm cells experience considerable post-ejaculation environmental variation. However, little is known about whether this affects their molecular composition, probably owing to the assumption that sperm are transcriptionally quiescent. Nevertheless, recent evidence shows have distinct RNA profiles affect fertilization and embryo viability. Moreover, RNAs expected be highly sensitive extracellular changes. One such group of heat shock protein (hsp) transcripts, which function in stress responses enriched sperm. Here, we exploit experimental tractability mussel Mytilus galloprovincialis by exposing paired samples ejaculated ambient (19°C) increased (25°C) temperatures, then measure (i) motility phenotypes, (ii) messenger (mRNA) levels two target genes ( hsp70 hsp90 ) several putative reference genes. We find no phenotypic changes motility, but reduced mRNA for gene gapdh at 25°C. This could reflect either decay specific RNAs, or translation degradation rates transcripts maintain under stress. These findings represent, our knowledge, first environments, suggest may more vulnerable from rising temperatures than currently thought.

Language: Английский

Citations

15
Adult exposure to acidified seawater influences sperm physiology in Mytilus galloprovincialis: Laboratory and in situ transplant experiments DOI
Alessandra Gallo, Maria Consiglia Esposito, Alessia Cuccaro

et al.

Environmental Pollution, Journal Year: 2020, Volume and Issue: 265, P. 115063 - 115063

Published: June 23, 2020

Language: Английский

Citations

14
Ocean acidification during prefertilization chemical communication affects sperm success DOI Creative Commons
Rowan A. Lymbery, W. Jason Kennington, Christopher E. Cornwall

et al.

Ecology and Evolution, Journal Year: 2019, Volume and Issue: 9(21), P. 12302 - 12310

Published: Oct. 16, 2019

Ocean acidification (OA) poses a major threat to marine organisms, particularly during reproduction when externally shed gametes are vulnerable changes in seawater pH. Accordingly, several studies on OA have focused how pH influence sperm behavior and/or rates of vitro fertilization. By contrast, few examined influences prefertilization gamete interactions, which crucial natural spawning events most fertilizing taxa. One mechanism interaction that forms an important component fertilization taxa is communication between and egg-derived chemicals. These chemical signals, along with the physiological responses they elicit, likely be highly sensitive chemistry. In this study, we experimentally tested possibility using blue mussel, Mytilus galloprovincialis, species females been shown use chemicals promote success from genetically compatible males. We conducted trials were allowed swim gradients under different CO2 (and therefore pH) treatments. found had elevated after swimming presence low (pH 7.6) compared ambient 8.0) seawater. This observed effect could implications for reproductive fitness external fertilizers, where compatibility plays critical role modulating many species. For example, might disrupt eggs' capacity avoid fertilizations by incompatible sperm. Our findings highlight need understand affects multiple stages sperm-egg interactions develop approaches disentangle female, male, population fitness.

Language: Английский

Citations

12
Impacts of Global Warming on Quality of Male Fishes: Evidence From A Meta‐Analysis of Temperature Effects on Sperm Motility Kinematics DOI Creative Commons
Yu Cheng, Jinhai Wang, Sayyed Mohammad Hadi Alavi

et al.

Reviews in Aquaculture, Journal Year: 2024, Volume and Issue: 17(1)

Published: Aug. 28, 2024

ABSTRACT Studies have demonstrated adverse effects of global warming on aquatic ecosystems. However, the increased water temperature (IWT) fish reproduction are still doubtful in species spawning at low or high temperatures. In this meta‐analysis, we elucidated spermatozoa functions, key determinants male fertility. We recruited 245 data records from 20 studies spanning cold‐ and warm‐water fishes to identify IWT sperm quality. Data were re‐processed re‐analyzed determine overall kinetics such as percentage motile (MOT), duration motility (DSM), curvilinear velocity (VCL), straight line (VSL), average path (VAP), well enzymatic activities for energy supply (EAES) antioxidant enzyme activity (ANEA). The standardized mean difference was calculated each study, with positive values indicating higher performance under IWT. Results showed that (a) effect size MOT larger negative cold‐water (−1.22) than (−0.95). (b) Each 1°C increase activation medium reduced by 1.30% (cold‐water fishes) 3.47% (warm‐water fishes). (c) negatively affected DSM, decreasing it 10 s species) 5.64 per degree (d) Spermatozoa (VCL VSL) species. (e) species, IWT‐induced detrimental EAES associated impacts kinetics. conclusion, affects kinetics, suggesting an impact reproduction.

Language: Английский

Citations

1
Ocean acidification alters sperm responses to egg-derived chemicals in a broadcast spawning mussel DOI Creative Commons
Rowan A. Lymbery,

Jill Brouwer,

Jonathan P. Evans

et al.

Biology Letters, Journal Year: 2022, Volume and Issue: 18(4)

Published: April 1, 2022

The continued emissions of anthropogenic carbon dioxide are causing progressive ocean acidification (OA). While deleterious effects OA on biological systems well documented in the growth calcifying organisms, lesser studied impacts include potential gamete interactions that determine fertilization, which likely to influence many marine species spawn gametes externally. Here, we explore signalling mechanisms enable sperm track egg-derived chemicals (sperm chemotaxis). We focus mussel

Language: Английский

Citations

5
Impacts of global warming on fertility of male fishes: evidence from meta-analysis of temperature effects on spermatozoa motility DOI Open Access
Yu Cheng, Jinhai Wang, Sayyed Mohammad Hadi Alavi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 28, 2024

Abstract Studies have demonstrated adverse effects of global warming on aquatic ecosystems from the abiotic to biotic level. In present work, a meta-analysis study was conducted elucidate spermatozoa functions, which are key determinants male fertility. We recruited 245 data records pool empirical studies, includes 20 studies spanning cold- and warm-water fish species, identify increased water temperature (IWT) sperm fertility in fishes. Data were systematically re-processed re-analyzed determine overall IWT kinetics such as motility (MOT), duration (DSM), curvilinear velocity (VCL), rectilinear (VSL) average path (VAP), well enzymatic activities for energy supply (EAES) antioxidant enzyme activity (ANEA). The standardized mean difference calculated each study, with positive values indicating higher performance under IWT. Results showed that (a) effect size MOT more negative cold-water fishes (-1.22) than (-0.98). (b) Each 1 °C increase activation medium reduced by 1.30% (cold-water fishes) 3.47% (warm-water fishes). (c) negatively affected DSM, decreasing it 10 s species) 5.64 per degree (d) Spermatozoa (VCL VSL) species. conclusion, this shows affects kinetics, suggesting an impact reproduction.

Language: Английский

Citations

0